Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green Dye
Simple, accurate and sensitive indirect spectrophotometric method for assay of diclofenac (Di) and Isoxsuprine (IS) in pure and pharmaceutical formulation has been developed. The method is based on oxidation of (Di) and (IS) by N-bromosuccinimide in acidic medium. The excess of oxidant is determine...
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College of Education for Pure Sciences
2019-12-01
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doaj-0d7e0e48916640e0988590fb6f8f56ec2020-11-25T02:28:49ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302019-12-01284193410.33899/edusj.1999.163311163311Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green DyeMarwan Al- SharookLeena Al-SalimSimple, accurate and sensitive indirect spectrophotometric method for assay of diclofenac (Di) and Isoxsuprine (IS) in pure and pharmaceutical formulation has been developed. The method is based on oxidation of (Di) and (IS) by N-bromosuccinimide in acidic medium. The excess of oxidant is determine by reaction with malachite green dye and the absorbance is measured at 615nm. Under the optimized experimental conditions, Beer's law is obeyed in concentration range 0.5-18 , 1-18 µg/ml with molar absorptivity 5.1×10<sup>4 </sup>and 4.6×10<sup>4 </sup>L.mol<sup>-1</sup>.cm<sup>-1</sup> for (Di) and (IS) respectively. The limits of detection (LOD) 0.0314, 0.038 and quantification are 0.0104 , 0.126 µg/ml. with the accuracy (average recovery) 100.80%, 101.20% for above drugs respectively and the precision (RSD) less than 1.5% for both drugs. The proposed method is applied successfully for assay of pharmaceutical formulations.https://edusj.mosuljournals.com/article_163311_2622c4764d13645a13ad6e4106724a46.pdfdiclofenacisoxsuprinemalachite greenspectrophotometry |
collection |
DOAJ |
language |
Arabic |
format |
Article |
sources |
DOAJ |
author |
Marwan Al- Sharook Leena Al-Salim |
spellingShingle |
Marwan Al- Sharook Leena Al-Salim Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green Dye مجلة التربية والعلم diclofenac isoxsuprine malachite green spectrophotometry |
author_facet |
Marwan Al- Sharook Leena Al-Salim |
author_sort |
Marwan Al- Sharook |
title |
Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green Dye |
title_short |
Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green Dye |
title_full |
Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green Dye |
title_fullStr |
Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green Dye |
title_full_unstemmed |
Indirect Spectrophotometric Determination of Diclofenac and Isoxsuprine Drugs Using N-bromosuccinimide and Malachite Green Dye |
title_sort |
indirect spectrophotometric determination of diclofenac and isoxsuprine drugs using n-bromosuccinimide and malachite green dye |
publisher |
College of Education for Pure Sciences |
series |
مجلة التربية والعلم |
issn |
1812-125X 2664-2530 |
publishDate |
2019-12-01 |
description |
Simple, accurate and sensitive indirect spectrophotometric method for assay of diclofenac (Di) and Isoxsuprine (IS) in pure and pharmaceutical formulation has been developed. The method is based on oxidation of (Di) and (IS) by N-bromosuccinimide in acidic medium. The excess of oxidant is determine by reaction with malachite green dye and the absorbance is measured at 615nm. Under the optimized experimental conditions, Beer's law is obeyed in concentration range 0.5-18 , 1-18 µg/ml with molar absorptivity 5.1×10<sup>4 </sup>and 4.6×10<sup>4 </sup>L.mol<sup>-1</sup>.cm<sup>-1</sup> for (Di) and (IS) respectively. The limits of detection (LOD) 0.0314, 0.038 and quantification are 0.0104 , 0.126 µg/ml. with the accuracy (average recovery) 100.80%, 101.20% for above drugs respectively and the precision (RSD) less than 1.5% for both drugs. The proposed method is applied successfully for assay of pharmaceutical formulations. |
topic |
diclofenac isoxsuprine malachite green spectrophotometry |
url |
https://edusj.mosuljournals.com/article_163311_2622c4764d13645a13ad6e4106724a46.pdf |
work_keys_str_mv |
AT marwanalsharook indirectspectrophotometricdeterminationofdiclofenacandisoxsuprinedrugsusingnbromosuccinimideandmalachitegreendye AT leenaalsalim indirectspectrophotometricdeterminationofdiclofenacandisoxsuprinedrugsusingnbromosuccinimideandmalachitegreendye |
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