The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head
Background: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role. Cordycepin benefits bone metabolism; however, there has been no study to determine its effect on osteonecrosis. Meth...
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Cell Physiol Biochem Press GmbH & Co KG
2017-08-01
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doaj-0dabeac90ae74e1993ee648dd0ef47e52020-11-25T00:50:43ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-08-014262391240310.1159/000480181480181The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral HeadYi-Xuan ChenDao-Yu ZhuZheng-Liang XuJun-Hui YinXiao-Wei YuJiong MeiYou-Shui GaoChang-Qing ZhangBackground: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role. Cordycepin benefits bone metabolism; however, there has been no study to determine its effect on osteonecrosis. Methods: Human bone mesenchymal stem cells (hBMSCs) were identified by multi-lineage differentiation. Alkaline phosphatase (ALP) activity, RT-PCR, western blots, immunofluorescent assay and Alizarin red staining of BMSCs were evaluated. A rat model of alcohol-induced ONFH was established to investigate the protective role of cordycepin against ethanol. Hematoxylin & eosin (H&E) staining and micro-computerized tomography (micro-CT) were performed to observe ONFH. Apoptosis was assessed by TdT-mediated dUTP nick end labeling (TUNEL). Immunohistochemical staining was carried out to detect OCN and COL1. Results: Ethanol significantly suppressed ALP activity, decreased gene expression of OCN and BMP2, lowered levels of RUNX2 protein, and reduced immunofluorescence staining of OCN and COL1 and calcium formation of hBMSCs. However, these inhibitory effects were attenuated by cordycepin co-treatment at concentrations of 1 and 10 µg/mL Moreover, it was revealed that the osteo-protective effect of cordycepin was associated with modulation of the Wnt/β-catenin pathway. In vivo, by micro-CT, TUNEL and immunohistochemical staining of OCN and COL1, we found that cordycepin administration prevented alcohol-induced ONFH. Conclusion: Cordycepin treatment to enhance osteogenesis may be considered a potential therapeutic approach to prevent the development of alcohol-induced ONFH.http://www.karger.com/Article/FullText/480181CordycepinOsteonecrosis of the femoral headAlcoholOsteogenesisWnt/β-catenin pathwayHuman bone mesenchymal stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi-Xuan Chen Dao-Yu Zhu Zheng-Liang Xu Jun-Hui Yin Xiao-Wei Yu Jiong Mei You-Shui Gao Chang-Qing Zhang |
spellingShingle |
Yi-Xuan Chen Dao-Yu Zhu Zheng-Liang Xu Jun-Hui Yin Xiao-Wei Yu Jiong Mei You-Shui Gao Chang-Qing Zhang The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head Cellular Physiology and Biochemistry Cordycepin Osteonecrosis of the femoral head Alcohol Osteogenesis Wnt/β-catenin pathway Human bone mesenchymal stem cells |
author_facet |
Yi-Xuan Chen Dao-Yu Zhu Zheng-Liang Xu Jun-Hui Yin Xiao-Wei Yu Jiong Mei You-Shui Gao Chang-Qing Zhang |
author_sort |
Yi-Xuan Chen |
title |
The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head |
title_short |
The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head |
title_full |
The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head |
title_fullStr |
The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head |
title_full_unstemmed |
The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head |
title_sort |
protective effect of cordycepin on alcohol-induced osteonecrosis of the femoral head |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2017-08-01 |
description |
Background: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role. Cordycepin benefits bone metabolism; however, there has been no study to determine its effect on osteonecrosis. Methods: Human bone mesenchymal stem cells (hBMSCs) were identified by multi-lineage differentiation. Alkaline phosphatase (ALP) activity, RT-PCR, western blots, immunofluorescent assay and Alizarin red staining of BMSCs were evaluated. A rat model of alcohol-induced ONFH was established to investigate the protective role of cordycepin against ethanol. Hematoxylin & eosin (H&E) staining and micro-computerized tomography (micro-CT) were performed to observe ONFH. Apoptosis was assessed by TdT-mediated dUTP nick end labeling (TUNEL). Immunohistochemical staining was carried out to detect OCN and COL1. Results: Ethanol significantly suppressed ALP activity, decreased gene expression of OCN and BMP2, lowered levels of RUNX2 protein, and reduced immunofluorescence staining of OCN and COL1 and calcium formation of hBMSCs. However, these inhibitory effects were attenuated by cordycepin co-treatment at concentrations of 1 and 10 µg/mL Moreover, it was revealed that the osteo-protective effect of cordycepin was associated with modulation of the Wnt/β-catenin pathway. In vivo, by micro-CT, TUNEL and immunohistochemical staining of OCN and COL1, we found that cordycepin administration prevented alcohol-induced ONFH. Conclusion: Cordycepin treatment to enhance osteogenesis may be considered a potential therapeutic approach to prevent the development of alcohol-induced ONFH. |
topic |
Cordycepin Osteonecrosis of the femoral head Alcohol Osteogenesis Wnt/β-catenin pathway Human bone mesenchymal stem cells |
url |
http://www.karger.com/Article/FullText/480181 |
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