Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.)
In the present study, we identified and characterized the apricot (Prunus armeniaca L.) homologs of three dormancy-related genes, namely the ParCBF1 (C-repeat binding factor), ParDAM5 (dormancy-associated MADS-BOX) and ParDAM6 genes. All highly conserved structural motifs and the 3D model of the DNA...
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doaj-0db7cb2f083c4154aa85ca2590c55d192020-11-25T00:57:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-04-011010.3389/fpls.2019.00402444557Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.)Eszter Balogh0Júlia Halász1Alexandra Soltész2Zsolt Erös-Honti3Ádám Gutermuth4László Szalay5Mária Höhn6Attila Vágújfalvi7Gábor Galiba8Gábor Galiba9Attila Hegedüs10Department of Genetics and Plant Breeding, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Genetics and Plant Breeding, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Plant Molecular Biology, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, HungaryDepartment of Botany and Soroksár Botanical Garden, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Genetics and Plant Breeding, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Pomology, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Botany and Soroksár Botanical Garden, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Plant Molecular Biology, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, HungaryDepartment of Plant Molecular Biology, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, HungaryFestetics Doctoral School, Georgikon Faculty, University of Pannonia, Keszthely, HungaryDepartment of Genetics and Plant Breeding, Faculty of Horticultural Science, Szent István University, Budapest, HungaryIn the present study, we identified and characterized the apricot (Prunus armeniaca L.) homologs of three dormancy-related genes, namely the ParCBF1 (C-repeat binding factor), ParDAM5 (dormancy-associated MADS-BOX) and ParDAM6 genes. All highly conserved structural motifs and the 3D model of the DNA-binding domain indicate an unimpaired DNA-binding ability of ParCBF1. A phylogenetic analysis showed that ParCBF1 was most likely homologous to Prunus mume and Prunus dulcis CBF1. ParDAM5 also contained all characteristic domains of the type II (MIKCC) subfamily of MADS-box transcription factors. The homology modeling of protein domains and a phylogenetic analysis of ParDAM5 suggest its functional integrity. The amino acid positions or small motifs that are diagnostic characteristics of DAM5 and DAM6 were determined. For ParDAM6, only a small part of the cDNA was sequenced, which was sufficient for the quantification of gene expression. The expression of ParCBF1 showed close association with decreasing ambient temperatures in autumn and winter. The expression levels of ParDAM5 and ParDAM6 changed according to CBF1 expression rates and the fulfillment of cultivar chilling requirements (CR). The concomitant decrease of gene expression with endodormancy release is consistent with a role of ParDAM5 and ParDAM6 genes in dormancy induction and maintenance. Cultivars with higher CR and delayed flowering time showed higher expression levels of ParDAM5 and ParDAM6 toward the end of endodormancy. Differences in the timing of anther developmental stages between early- and late-flowering cultivars and two dormant seasons confirmed the genetically and environmentally controlled mechanisms of dormancy release in apricot generative buds. These results support that the newly identified apricot gene homologs have a crucial role in dormancy-associated physiological mechanisms.https://www.frontiersin.org/article/10.3389/fpls.2019.00402/fullapricotCBFDAMdormancyflower developmentgene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eszter Balogh Júlia Halász Alexandra Soltész Zsolt Erös-Honti Ádám Gutermuth László Szalay Mária Höhn Attila Vágújfalvi Gábor Galiba Gábor Galiba Attila Hegedüs |
spellingShingle |
Eszter Balogh Júlia Halász Alexandra Soltész Zsolt Erös-Honti Ádám Gutermuth László Szalay Mária Höhn Attila Vágújfalvi Gábor Galiba Gábor Galiba Attila Hegedüs Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.) Frontiers in Plant Science apricot CBF DAM dormancy flower development gene expression |
author_facet |
Eszter Balogh Júlia Halász Alexandra Soltész Zsolt Erös-Honti Ádám Gutermuth László Szalay Mária Höhn Attila Vágújfalvi Gábor Galiba Gábor Galiba Attila Hegedüs |
author_sort |
Eszter Balogh |
title |
Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.) |
title_short |
Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.) |
title_full |
Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.) |
title_fullStr |
Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.) |
title_full_unstemmed |
Identification, Structural and Functional Characterization of Dormancy Regulator Genes in Apricot (Prunus armeniaca L.) |
title_sort |
identification, structural and functional characterization of dormancy regulator genes in apricot (prunus armeniaca l.) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2019-04-01 |
description |
In the present study, we identified and characterized the apricot (Prunus armeniaca L.) homologs of three dormancy-related genes, namely the ParCBF1 (C-repeat binding factor), ParDAM5 (dormancy-associated MADS-BOX) and ParDAM6 genes. All highly conserved structural motifs and the 3D model of the DNA-binding domain indicate an unimpaired DNA-binding ability of ParCBF1. A phylogenetic analysis showed that ParCBF1 was most likely homologous to Prunus mume and Prunus dulcis CBF1. ParDAM5 also contained all characteristic domains of the type II (MIKCC) subfamily of MADS-box transcription factors. The homology modeling of protein domains and a phylogenetic analysis of ParDAM5 suggest its functional integrity. The amino acid positions or small motifs that are diagnostic characteristics of DAM5 and DAM6 were determined. For ParDAM6, only a small part of the cDNA was sequenced, which was sufficient for the quantification of gene expression. The expression of ParCBF1 showed close association with decreasing ambient temperatures in autumn and winter. The expression levels of ParDAM5 and ParDAM6 changed according to CBF1 expression rates and the fulfillment of cultivar chilling requirements (CR). The concomitant decrease of gene expression with endodormancy release is consistent with a role of ParDAM5 and ParDAM6 genes in dormancy induction and maintenance. Cultivars with higher CR and delayed flowering time showed higher expression levels of ParDAM5 and ParDAM6 toward the end of endodormancy. Differences in the timing of anther developmental stages between early- and late-flowering cultivars and two dormant seasons confirmed the genetically and environmentally controlled mechanisms of dormancy release in apricot generative buds. These results support that the newly identified apricot gene homologs have a crucial role in dormancy-associated physiological mechanisms. |
topic |
apricot CBF DAM dormancy flower development gene expression |
url |
https://www.frontiersin.org/article/10.3389/fpls.2019.00402/full |
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