Double staining method for array tomography using scanning electron microscopy
Abstract Scanning electron microscopy (SEM) plays a central role in analyzing structures by imaging a large area of brain tissue at nanometer scales. A vast amount of data in the large area are required to study structural changes of cellular organelles in a specific cell, such as neurons, astrocyte...
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Online Access: | https://doi.org/10.1186/s42649-020-00033-8 |
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doaj-0dbac22035b549178c83159b6cf2e0602021-06-27T11:25:39ZengSpringerOpenApplied Microscopy2287-44452020-06-015011610.1186/s42649-020-00033-8Double staining method for array tomography using scanning electron microscopyEunjin Kim0Jiyoung Lee1Seulgi Noh2Ohkyung Kwon3Ji Young Mun4National Instrumentation Center for Environmental Management, Seoul National UniversityNational Instrumentation Center for Environmental Management, Seoul National UniversityDepartment of Brain and Cognitive Sciences, Daegu Gyeongbuk Institute of Science & Technology (DGIST)National Instrumentation Center for Environmental Management, Seoul National UniversityNeural circuit research group, Korea Brain Research InstituteAbstract Scanning electron microscopy (SEM) plays a central role in analyzing structures by imaging a large area of brain tissue at nanometer scales. A vast amount of data in the large area are required to study structural changes of cellular organelles in a specific cell, such as neurons, astrocytes, oligodendrocytes, and microglia among brain tissue, at sufficient resolution. Array tomography is a useful method for large-area imaging, and the osmium-thiocarbohydrazide-osmium (OTO) and ferrocyanide-reduced osmium methods are commonly used to enhance membrane contrast. Because many samples prepared using the conventional technique without en bloc staining are considered inadequate for array tomography, we suggested an alternative technique using post-staining conventional samples and compared the advantages.https://doi.org/10.1186/s42649-020-00033-8Array tomographyDouble staining with uranyl acetate and leadScanning electron microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eunjin Kim Jiyoung Lee Seulgi Noh Ohkyung Kwon Ji Young Mun |
spellingShingle |
Eunjin Kim Jiyoung Lee Seulgi Noh Ohkyung Kwon Ji Young Mun Double staining method for array tomography using scanning electron microscopy Applied Microscopy Array tomography Double staining with uranyl acetate and lead Scanning electron microscopy |
author_facet |
Eunjin Kim Jiyoung Lee Seulgi Noh Ohkyung Kwon Ji Young Mun |
author_sort |
Eunjin Kim |
title |
Double staining method for array tomography using scanning electron microscopy |
title_short |
Double staining method for array tomography using scanning electron microscopy |
title_full |
Double staining method for array tomography using scanning electron microscopy |
title_fullStr |
Double staining method for array tomography using scanning electron microscopy |
title_full_unstemmed |
Double staining method for array tomography using scanning electron microscopy |
title_sort |
double staining method for array tomography using scanning electron microscopy |
publisher |
SpringerOpen |
series |
Applied Microscopy |
issn |
2287-4445 |
publishDate |
2020-06-01 |
description |
Abstract Scanning electron microscopy (SEM) plays a central role in analyzing structures by imaging a large area of brain tissue at nanometer scales. A vast amount of data in the large area are required to study structural changes of cellular organelles in a specific cell, such as neurons, astrocytes, oligodendrocytes, and microglia among brain tissue, at sufficient resolution. Array tomography is a useful method for large-area imaging, and the osmium-thiocarbohydrazide-osmium (OTO) and ferrocyanide-reduced osmium methods are commonly used to enhance membrane contrast. Because many samples prepared using the conventional technique without en bloc staining are considered inadequate for array tomography, we suggested an alternative technique using post-staining conventional samples and compared the advantages. |
topic |
Array tomography Double staining with uranyl acetate and lead Scanning electron microscopy |
url |
https://doi.org/10.1186/s42649-020-00033-8 |
work_keys_str_mv |
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