Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells
Treatment of human breast epithelial cells MCF-10F with 17-β-estradiol has been reported to result in E2-transformed cells which have given rise to highly invasive C5 cells that in turn generate tumors in SCID mice. From these tumors, various cell lines, among which C5-A6-T6 and C5-A8-T8, were obtai...
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doaj-0dd1e15bb4814901b4d2da912e422f402020-11-24T23:42:43ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-437571248749010.1590/S1519-69842011000300019S1519-69842011000300019Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cellsLMS Cruz0JCV Ferreira1MLS Mello2Universidade Estadual de CampinasUniversidade Estadual de CampinasUniversidade Estadual de CampinasTreatment of human breast epithelial cells MCF-10F with 17-β-estradiol has been reported to result in E2-transformed cells which have given rise to highly invasive C5 cells that in turn generate tumors in SCID mice. From these tumors, various cell lines, among which C5-A6-T6 and C5-A8-T8, were obtained. Although different phases of the tumorigenesis process in this model have been studied in molecular biology and image analysis assays, no cytological data on apoptotic ratios and mitotic abnormalities have been established to accompany the various steps leading to 17-β-estradiol-treated MCF-10F cells to tumorigenesis. Here we detected that the apoptotic ratio decreases with the transformation and tumorigenesis progress, except for the tumor cell line C5-A8-T8, probably on account of its more intense proliferation rate and a more rapid culture medium consumption. Increased frequency of mitotic abnormalities contributed by triple- and tetrapolar metaphases, and by lagging chromosomes and chromosome bridges observed at the anaphase found by transformation and tumorigenesis progress. However, no difference was found under these terms when the C5-A6-T6 and C5-A8-T8 tumor cell lines were compared to each other. Present findings are in agreement with the nuclear instability and enrichment of dysregulated genes in the apoptotic process promoted by transformation and tumorigenesis in 17-β-estradiol-treated MCF-10F cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842011000300019&lng=en&tlng=enMCF-10F cellsestradioltransformationapoptotic ratiosmitotic abnormalities |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
LMS Cruz JCV Ferreira MLS Mello |
spellingShingle |
LMS Cruz JCV Ferreira MLS Mello Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells Brazilian Journal of Biology MCF-10F cells estradiol transformation apoptotic ratios mitotic abnormalities |
author_facet |
LMS Cruz JCV Ferreira MLS Mello |
author_sort |
LMS Cruz |
title |
Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells |
title_short |
Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells |
title_full |
Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells |
title_fullStr |
Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells |
title_full_unstemmed |
Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells |
title_sort |
apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial mcf-10f cells |
publisher |
Instituto Internacional de Ecologia |
series |
Brazilian Journal of Biology |
issn |
1678-4375 |
description |
Treatment of human breast epithelial cells MCF-10F with 17-β-estradiol has been reported to result in E2-transformed cells which have given rise to highly invasive C5 cells that in turn generate tumors in SCID mice. From these tumors, various cell lines, among which C5-A6-T6 and C5-A8-T8, were obtained. Although different phases of the tumorigenesis process in this model have been studied in molecular biology and image analysis assays, no cytological data on apoptotic ratios and mitotic abnormalities have been established to accompany the various steps leading to 17-β-estradiol-treated MCF-10F cells to tumorigenesis. Here we detected that the apoptotic ratio decreases with the transformation and tumorigenesis progress, except for the tumor cell line C5-A8-T8, probably on account of its more intense proliferation rate and a more rapid culture medium consumption. Increased frequency of mitotic abnormalities contributed by triple- and tetrapolar metaphases, and by lagging chromosomes and chromosome bridges observed at the anaphase found by transformation and tumorigenesis progress. However, no difference was found under these terms when the C5-A6-T6 and C5-A8-T8 tumor cell lines were compared to each other. Present findings are in agreement with the nuclear instability and enrichment of dysregulated genes in the apoptotic process promoted by transformation and tumorigenesis in 17-β-estradiol-treated MCF-10F cells. |
topic |
MCF-10F cells estradiol transformation apoptotic ratios mitotic abnormalities |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842011000300019&lng=en&tlng=en |
work_keys_str_mv |
AT lmscruz apoptoticratiosandmitoticabnormalitiesin17bestradioltransformedhumanbreastepithelialmcf10fcells AT jcvferreira apoptoticratiosandmitoticabnormalitiesin17bestradioltransformedhumanbreastepithelialmcf10fcells AT mlsmello apoptoticratiosandmitoticabnormalitiesin17bestradioltransformedhumanbreastepithelialmcf10fcells |
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1725503345731305472 |