Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells
Human amniotic fluid stem cells (AFSC) are an attractive source for cell therapy due to their multilineage differentiation potential and accessibility advantages. However the clinical application of human stem cells largely depends on their capacity to expand in vitro, since there is an extensive do...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2015-01-01
|
Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2015/101304 |
id |
doaj-0dde66c9d5d74fd7b7b5caad364b019b |
---|---|
record_format |
Article |
spelling |
doaj-0dde66c9d5d74fd7b7b5caad364b019b2020-11-24T23:23:04ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942015-01-01201510.1155/2015/101304101304Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem CellsTullia Maraldi0Marianna Guida1Manuela Zavatti2Elisa Resca3Laura Bertoni4Giovanni B. La Sala5Anto De Pol6Department of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via Del Pozzo 71, 41100 Modena, ItalyDepartment of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via Del Pozzo 71, 41100 Modena, ItalyDepartment of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via Del Pozzo 71, 41100 Modena, ItalyDepartment of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via Del Pozzo 71, 41100 Modena, ItalyDepartment of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via Del Pozzo 71, 41100 Modena, ItalyUnit of Obstetrics & Gynecology, IRCCS-Arcispedale Santa Maria Nuova, Viale Umberto I 50, 42123 Reggio Emilia, ItalyDepartment of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via Del Pozzo 71, 41100 Modena, ItalyHuman amniotic fluid stem cells (AFSC) are an attractive source for cell therapy due to their multilineage differentiation potential and accessibility advantages. However the clinical application of human stem cells largely depends on their capacity to expand in vitro, since there is an extensive donor-to-donor heterogeneity. Reactive oxygen species (ROS) and cellular oxidative stress are involved in many physiological and pathophysiological processes of stem cells, including pluripotency, proliferation, differentiation, and stress resistance. The mode of action of ROS is also dependent on the localization of their target molecules. Thus, the modifications induced by ROS can be separated depending on the cellular compartments they affect. NAD(P)H oxidase family, particularly Nox4, has been known to produce ROS in the nucleus. In the present study we show that Nox4 nuclear expression (nNox4) depends on the donor and it correlates with the expression of transcription factors involved in stemness regulation, such as Oct4, SSEA-4, and Sox2. Moreover nNox4 is linked with the nuclear localization of redox sensitive transcription factors, as Nrf2 and NF-κB, and with the differentiation potential. Taken together, these results suggest that nNox4 regulation may have important effects in stem cell capability through modulation of transcription factors and DNA damage.http://dx.doi.org/10.1155/2015/101304 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tullia Maraldi Marianna Guida Manuela Zavatti Elisa Resca Laura Bertoni Giovanni B. La Sala Anto De Pol |
spellingShingle |
Tullia Maraldi Marianna Guida Manuela Zavatti Elisa Resca Laura Bertoni Giovanni B. La Sala Anto De Pol Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells Oxidative Medicine and Cellular Longevity |
author_facet |
Tullia Maraldi Marianna Guida Manuela Zavatti Elisa Resca Laura Bertoni Giovanni B. La Sala Anto De Pol |
author_sort |
Tullia Maraldi |
title |
Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells |
title_short |
Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells |
title_full |
Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells |
title_fullStr |
Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells |
title_full_unstemmed |
Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells |
title_sort |
nuclear nox4 role in stemness power of human amniotic fluid stem cells |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2015-01-01 |
description |
Human amniotic fluid stem cells (AFSC) are an attractive source for cell therapy due to their multilineage differentiation potential and accessibility advantages. However the clinical application of human stem cells largely depends on their capacity to expand in vitro, since there is an extensive donor-to-donor heterogeneity. Reactive oxygen species (ROS) and cellular oxidative stress are involved in many physiological and pathophysiological processes of stem cells, including pluripotency, proliferation, differentiation, and stress resistance. The mode of action of ROS is also dependent on the localization of their target molecules. Thus, the modifications induced by ROS can be separated depending on the cellular compartments they affect. NAD(P)H oxidase family, particularly Nox4, has been known to produce ROS in the nucleus. In the present study we show that Nox4 nuclear expression (nNox4) depends on the donor and it correlates with the expression of transcription factors involved in stemness regulation, such as Oct4, SSEA-4, and Sox2. Moreover nNox4 is linked with the nuclear localization of redox sensitive transcription factors, as Nrf2 and NF-κB, and with the differentiation potential. Taken together, these results suggest that nNox4 regulation may have important effects in stem cell capability through modulation of transcription factors and DNA damage. |
url |
http://dx.doi.org/10.1155/2015/101304 |
work_keys_str_mv |
AT tulliamaraldi nuclearnox4roleinstemnesspowerofhumanamnioticfluidstemcells AT mariannaguida nuclearnox4roleinstemnesspowerofhumanamnioticfluidstemcells AT manuelazavatti nuclearnox4roleinstemnesspowerofhumanamnioticfluidstemcells AT elisaresca nuclearnox4roleinstemnesspowerofhumanamnioticfluidstemcells AT laurabertoni nuclearnox4roleinstemnesspowerofhumanamnioticfluidstemcells AT giovanniblasala nuclearnox4roleinstemnesspowerofhumanamnioticfluidstemcells AT antodepol nuclearnox4roleinstemnesspowerofhumanamnioticfluidstemcells |
_version_ |
1725565549672398848 |