Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells

Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells

Summary: The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humoral immune responses. Here, we describe a protocol for the sequencing, cloning, and characterization of antibody genes that encode BCRs. We used this method to analyze the BCRs of different mouse B ce...

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Bibliographic Details
Main Authors: Charlotte Viant, Amelia Escolano, Spencer T. Chen, Michel C. Nussenzweig
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell isolation
Single cell
Sequencing
Immunology
Molecular biology
Antibody
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000964

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