Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells
Summary: The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humoral immune responses. Here, we describe a protocol for the sequencing, cloning, and characterization of antibody genes that encode BCRs. We used this method to analyze the BCRs of different mouse B ce...
Main Authors: | Charlotte Viant, Amelia Escolano, Spencer T. Chen, Michel C. Nussenzweig |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000964 |
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