The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments
The sheep intestinal tract is characterized by a diverse microbial ecosystem that is vital for the host to digest diet material. The importance of gut microbiota (GM) of animals has also been widely acknowledged because of its pivotal roles in the health and well-being of animals. However, there are...
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Frontiers Media S.A.
2018-01-01
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Series: | Frontiers in Microbiology |
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Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00032/full |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hao Zhang Hao Zhang Mingxu Shao He Huang Shujuan Wang Shujuan Wang Lili Ma Lili Ma Huining Wang Huining Wang Liping Hu Kai Wei Kai Wei Ruiliang Zhu Ruiliang Zhu |
spellingShingle |
Hao Zhang Hao Zhang Mingxu Shao He Huang Shujuan Wang Shujuan Wang Lili Ma Lili Ma Huining Wang Huining Wang Liping Hu Kai Wei Kai Wei Ruiliang Zhu Ruiliang Zhu The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments Frontiers in Microbiology gut microbiota small-tail Han sheep high-throughput sequencing dynamic distribution volatile fatty acids |
author_facet |
Hao Zhang Hao Zhang Mingxu Shao He Huang Shujuan Wang Shujuan Wang Lili Ma Lili Ma Huining Wang Huining Wang Liping Hu Kai Wei Kai Wei Ruiliang Zhu Ruiliang Zhu |
author_sort |
Hao Zhang |
title |
The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments |
title_short |
The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments |
title_full |
The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments |
title_fullStr |
The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments |
title_full_unstemmed |
The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments |
title_sort |
dynamic distribution of small-tail han sheep microbiota across different intestinal segments |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-01-01 |
description |
The sheep intestinal tract is characterized by a diverse microbial ecosystem that is vital for the host to digest diet material. The importance of gut microbiota (GM) of animals has also been widely acknowledged because of its pivotal roles in the health and well-being of animals. However, there are no relevant studies on GM of small-tail Han sheep, a superior mutton variety domestic in China. In this study, the structure and distribution of gut microflora were studied by high-throughput sequencing technology. Results showed a significant difference between jejunum and cecum, jejunum, and rectum. Meanwhile, the cecum and rectum not only display higher species richness but also exhibit higher similarity of the bacterial diversity than that of the jejunum based on the results of abundance-based coverage estimator (ACE), Chao1, and Shannon indexes. Firmicutes and Bacteroidetes were the predominant phyla in cecum and rectum, while higher relative abundances of Firmicutes and Cyanobacteria were observed in jejunum. At the genus level, Bacteroidetes, Ruminococcus, Lactobacillus, Flavonifractor, and Clostridium were the dominant genera in the cecum and rectum. An obvious dynamic distribution of Lactobacillus is continuously decreasing from the jejunum to the cecum, then to the rectum, whereas the result of Bacteroides is completely inverse. In addition, this study also found many kinds of bacteria associated with the production of volatile fatty acids (VFA) colonized in the large intestine. This study is the first to investigate the distribution of intestinal flora in small-tail Han sheep. The findings provide an important indication for diagnosis and treatment of intestinal diseases in small-tail Han sheep, as well as offer a direction for the development of intestinal microecological preparations. |
topic |
gut microbiota small-tail Han sheep high-throughput sequencing dynamic distribution volatile fatty acids |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00032/full |
work_keys_str_mv |
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doaj-0dfc56f8caaa4688a68ef3ad8ebff3bc2020-11-25T01:06:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-01-01910.3389/fmicb.2018.00032303700The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal SegmentsHao Zhang0Hao Zhang1Mingxu Shao2He Huang3Shujuan Wang4Shujuan Wang5Lili Ma6Lili Ma7Huining Wang8Huining Wang9Liping Hu10Kai Wei11Kai Wei12Ruiliang Zhu13Ruiliang Zhu14Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaLianyungang Entry-Exit Inspection and Quarantine Bureau, Lianyungang, ChinaShandong New Hope Liuhe Co., Ltd., New Hope Group, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaAnimal Disease Prevention and Control Center of Shandong Province, Animal Husbandry and Veterinary Bureau of Shandong Province, Jinan, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaThe sheep intestinal tract is characterized by a diverse microbial ecosystem that is vital for the host to digest diet material. The importance of gut microbiota (GM) of animals has also been widely acknowledged because of its pivotal roles in the health and well-being of animals. However, there are no relevant studies on GM of small-tail Han sheep, a superior mutton variety domestic in China. In this study, the structure and distribution of gut microflora were studied by high-throughput sequencing technology. Results showed a significant difference between jejunum and cecum, jejunum, and rectum. Meanwhile, the cecum and rectum not only display higher species richness but also exhibit higher similarity of the bacterial diversity than that of the jejunum based on the results of abundance-based coverage estimator (ACE), Chao1, and Shannon indexes. Firmicutes and Bacteroidetes were the predominant phyla in cecum and rectum, while higher relative abundances of Firmicutes and Cyanobacteria were observed in jejunum. At the genus level, Bacteroidetes, Ruminococcus, Lactobacillus, Flavonifractor, and Clostridium were the dominant genera in the cecum and rectum. An obvious dynamic distribution of Lactobacillus is continuously decreasing from the jejunum to the cecum, then to the rectum, whereas the result of Bacteroides is completely inverse. In addition, this study also found many kinds of bacteria associated with the production of volatile fatty acids (VFA) colonized in the large intestine. This study is the first to investigate the distribution of intestinal flora in small-tail Han sheep. The findings provide an important indication for diagnosis and treatment of intestinal diseases in small-tail Han sheep, as well as offer a direction for the development of intestinal microecological preparations.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00032/fullgut microbiotasmall-tail Han sheephigh-throughput sequencingdynamic distributionvolatile fatty acids |