The effect of inversion at 8p23 on BLK association with lupus in Caucasian population.
UNLABELLED:To explore the potential influence of the polymorphic 8p23.1 inversion on known autoimmune susceptibility risk at or near BLK locus, we validated a new bioinformatics method that utilizes SNP data to enable accurate, high-throughput genotyping of the 8p23.1 inversion in a Caucasian popula...
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doaj-0dfd226d636948cfad0433d4aa3e4eed2020-11-25T01:25:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11561410.1371/journal.pone.0115614The effect of inversion at 8p23 on BLK association with lupus in Caucasian population.Bahram NamjouYizhao NiIsaac T W HarleyIouri ChepelevBeth CobbLeah C KottyanPatrick M GaffneyJoel M GuthridgeKenneth KaufmanJohn B HarleyUNLABELLED:To explore the potential influence of the polymorphic 8p23.1 inversion on known autoimmune susceptibility risk at or near BLK locus, we validated a new bioinformatics method that utilizes SNP data to enable accurate, high-throughput genotyping of the 8p23.1 inversion in a Caucasian population. METHODS:Principal components analysis (PCA) was performed using markers inside the inversion territory followed by k-means cluster analyses on 7416 European derived and 267 HapMaP CEU and TSI samples. A logistic regression conditional analysis was performed. RESULTS:Three subgroups have been identified; inversion homozygous, heterozygous and non-inversion homozygous. The status of inversion was further validated using HapMap samples that had previously undergone Fluorescence in situ hybridization (FISH) assays with a concordance rate of above 98%. Conditional analyses based on the status of inversion were performed. We found that overall association signals in the BLK region remain significant after controlling for inversion status. The proportion of lupus cases and controls (cases/controls) in each subgroup was determined to be 0.97 for the inverted homozygous group (1067 cases and 1095 controls), 1.12 for the inverted heterozygous group (1935 cases 1717 controls) and 1.36 for non-inverted subgroups (924 cases and 678 controls). After calculating the linkage disequilibrium between inversion status and lupus risk haplotype we found that the lupus risk haplotype tends to reside on non-inversion background. As a result, a new association effect between non-inversion status and lupus phenotype has been identified ((p = 8.18×10(-7), OR = 1.18, 95%CI = 1.10-1.26). CONCLUSION:Our results demonstrate that both known lupus risk haplotype and inversion status act additively in the pathogenesis of lupus. Since inversion regulates expression of many genes in its territory, altered expression of other genes might also be involved in the development of lupus.http://europepmc.org/articles/PMC4278715?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bahram Namjou Yizhao Ni Isaac T W Harley Iouri Chepelev Beth Cobb Leah C Kottyan Patrick M Gaffney Joel M Guthridge Kenneth Kaufman John B Harley |
spellingShingle |
Bahram Namjou Yizhao Ni Isaac T W Harley Iouri Chepelev Beth Cobb Leah C Kottyan Patrick M Gaffney Joel M Guthridge Kenneth Kaufman John B Harley The effect of inversion at 8p23 on BLK association with lupus in Caucasian population. PLoS ONE |
author_facet |
Bahram Namjou Yizhao Ni Isaac T W Harley Iouri Chepelev Beth Cobb Leah C Kottyan Patrick M Gaffney Joel M Guthridge Kenneth Kaufman John B Harley |
author_sort |
Bahram Namjou |
title |
The effect of inversion at 8p23 on BLK association with lupus in Caucasian population. |
title_short |
The effect of inversion at 8p23 on BLK association with lupus in Caucasian population. |
title_full |
The effect of inversion at 8p23 on BLK association with lupus in Caucasian population. |
title_fullStr |
The effect of inversion at 8p23 on BLK association with lupus in Caucasian population. |
title_full_unstemmed |
The effect of inversion at 8p23 on BLK association with lupus in Caucasian population. |
title_sort |
effect of inversion at 8p23 on blk association with lupus in caucasian population. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
UNLABELLED:To explore the potential influence of the polymorphic 8p23.1 inversion on known autoimmune susceptibility risk at or near BLK locus, we validated a new bioinformatics method that utilizes SNP data to enable accurate, high-throughput genotyping of the 8p23.1 inversion in a Caucasian population. METHODS:Principal components analysis (PCA) was performed using markers inside the inversion territory followed by k-means cluster analyses on 7416 European derived and 267 HapMaP CEU and TSI samples. A logistic regression conditional analysis was performed. RESULTS:Three subgroups have been identified; inversion homozygous, heterozygous and non-inversion homozygous. The status of inversion was further validated using HapMap samples that had previously undergone Fluorescence in situ hybridization (FISH) assays with a concordance rate of above 98%. Conditional analyses based on the status of inversion were performed. We found that overall association signals in the BLK region remain significant after controlling for inversion status. The proportion of lupus cases and controls (cases/controls) in each subgroup was determined to be 0.97 for the inverted homozygous group (1067 cases and 1095 controls), 1.12 for the inverted heterozygous group (1935 cases 1717 controls) and 1.36 for non-inverted subgroups (924 cases and 678 controls). After calculating the linkage disequilibrium between inversion status and lupus risk haplotype we found that the lupus risk haplotype tends to reside on non-inversion background. As a result, a new association effect between non-inversion status and lupus phenotype has been identified ((p = 8.18×10(-7), OR = 1.18, 95%CI = 1.10-1.26). CONCLUSION:Our results demonstrate that both known lupus risk haplotype and inversion status act additively in the pathogenesis of lupus. Since inversion regulates expression of many genes in its territory, altered expression of other genes might also be involved in the development of lupus. |
url |
http://europepmc.org/articles/PMC4278715?pdf=render |
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