Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries
Antimicrobials are important adjuncts in the treatment of caries and periodontitis. However, increased bacterial resistance and hypersensitivity reactions to commonly used antimicrobials have led to an increasing demand for safe and natural substances. The objective of this study was to investigate...
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doaj-0e11ab9e35f043b9ae6e1879452003a62020-11-25T03:15:04ZengMDPI AGPolymers2073-43602020-05-01121218121810.3390/polym12061218Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and CariesShinechimeg Dima0Yin-Yin Lee1Ikki Watanabe2Wei-Jen Chang3Yu-Hua Pan4Nai-Chia Teng5School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (R.O.C.)School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (R.O.C.)Gerodontology and Oral Rehabilitation, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8510, JapanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (R.O.C.)School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (R.O.C.)School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (R.O.C.)Antimicrobials are important adjuncts in the treatment of caries and periodontitis. However, increased bacterial resistance and hypersensitivity reactions to commonly used antimicrobials have led to an increasing demand for safe and natural substances. The objective of this study was to investigate the antibacterial effects of ε-polylysine against oral pathogens <i>Streptococcus mutans</i> and <i>Porphyromonas gingivalis</i>. Broth dilution assay, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analyses were performed to explore the antibacterial effect of ε-polylysine against <i>S. mutans</i> strain ATCC25175 and <i>P. gingivalis</i> strain ATCC332277. For the test solution, ε-polylysine was added to the bacterial suspension to prepare 0.125%, 0.25%, 0.5% and 1% ε-polylysine solutions diluted in broth medium. All four concentrations demonstrated complete inhibition of <i>S. mutans</i> and significantly reduced viable cell counts of <i>P. gingivalis</i> after 24 h. From starting inoculum of 9.15 log CFU/mL, <i>P. gingivalis</i> cell counts reduced to 4.01 log CFU/mL in the 0.125% ε-polylysine treatment group. SEM, CLSM, and the LIVE/DEAD bacterial assay of ε-polylysine application on <i>P. gingivalis</i> biofilm-dentin specimens revealed bacterial cell membrane disruption and irregular cell morphologies. The results indicated satisfactory antibacterial efficacy of ε-polylysine against <i>P. gingivalis</i> and <i>S. mutans</i> in liquid medium and as an application on biofilm-dentin specimens.https://www.mdpi.com/2073-4360/12/6/1218polylysineantibacterialpolypeptide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shinechimeg Dima Yin-Yin Lee Ikki Watanabe Wei-Jen Chang Yu-Hua Pan Nai-Chia Teng |
spellingShingle |
Shinechimeg Dima Yin-Yin Lee Ikki Watanabe Wei-Jen Chang Yu-Hua Pan Nai-Chia Teng Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries Polymers polylysine antibacterial polypeptide |
author_facet |
Shinechimeg Dima Yin-Yin Lee Ikki Watanabe Wei-Jen Chang Yu-Hua Pan Nai-Chia Teng |
author_sort |
Shinechimeg Dima |
title |
Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries |
title_short |
Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries |
title_full |
Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries |
title_fullStr |
Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries |
title_full_unstemmed |
Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries |
title_sort |
antibacterial effect of the natural polymer ε-polylysine against oral pathogens associated with periodontitis and caries |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2020-05-01 |
description |
Antimicrobials are important adjuncts in the treatment of caries and periodontitis. However, increased bacterial resistance and hypersensitivity reactions to commonly used antimicrobials have led to an increasing demand for safe and natural substances. The objective of this study was to investigate the antibacterial effects of ε-polylysine against oral pathogens <i>Streptococcus mutans</i> and <i>Porphyromonas gingivalis</i>. Broth dilution assay, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analyses were performed to explore the antibacterial effect of ε-polylysine against <i>S. mutans</i> strain ATCC25175 and <i>P. gingivalis</i> strain ATCC332277. For the test solution, ε-polylysine was added to the bacterial suspension to prepare 0.125%, 0.25%, 0.5% and 1% ε-polylysine solutions diluted in broth medium. All four concentrations demonstrated complete inhibition of <i>S. mutans</i> and significantly reduced viable cell counts of <i>P. gingivalis</i> after 24 h. From starting inoculum of 9.15 log CFU/mL, <i>P. gingivalis</i> cell counts reduced to 4.01 log CFU/mL in the 0.125% ε-polylysine treatment group. SEM, CLSM, and the LIVE/DEAD bacterial assay of ε-polylysine application on <i>P. gingivalis</i> biofilm-dentin specimens revealed bacterial cell membrane disruption and irregular cell morphologies. The results indicated satisfactory antibacterial efficacy of ε-polylysine against <i>P. gingivalis</i> and <i>S. mutans</i> in liquid medium and as an application on biofilm-dentin specimens. |
topic |
polylysine antibacterial polypeptide |
url |
https://www.mdpi.com/2073-4360/12/6/1218 |
work_keys_str_mv |
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