Sphingolipid distribution changes with age in the human lens[S]
The formation of an internal barrier to the diffusion of small molecules in the lens during middle age is hypothesized to be a key event in the development of age-related nuclear (ARN) cataract. Changes in membrane lipids with age may be responsible. In this study, we investigated the effect of age...
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doaj-0e13eedb79194df08944aa0210cb41712021-04-28T06:06:18ZengElsevierJournal of Lipid Research0022-22752010-09-0151927532760Sphingolipid distribution changes with age in the human lens[S]Jane M. Deeley0Joseph A. Hankin1Michael G. Friedrich2Robert C. Murphy3Roger J.W. Truscott4Todd W. Mitchell5Stephen J. Blanksby6Schools of Chemistry, University of Wollongong, Wollongong, AustraliaDepartment of Pharmacology, University of Colorado Health Sciences Centre, Aurora, COSave Sight Institute, University of Sydney, Sydney, AustraliaDepartment of Pharmacology, University of Colorado Health Sciences Centre, Aurora, COSave Sight Institute, University of Sydney, Sydney, AustraliaTo whom correspondence should be addressed. (S.J.B.); (T.W.M.). blanksby@uow.edu.au toddm@uow.edu.au; Health Sciences, University of Wollongong, Wollongong, AustraliaTo whom correspondence should be addressed. (S.J.B.); (T.W.M.). blanksby@uow.edu.au toddm@uow.edu.au; Schools of Chemistry, University of Wollongong, Wollongong, AustraliaThe formation of an internal barrier to the diffusion of small molecules in the lens during middle age is hypothesized to be a key event in the development of age-related nuclear (ARN) cataract. Changes in membrane lipids with age may be responsible. In this study, we investigated the effect of age on the distribution of sphingomyelins, the most abundant lens phospholipids. Human lens sections were initially analyzed by MALDI mass spectrometry imaging. A distinct annular distribution of the dihydrosphingomyelin, DHSM (d18:0/16:0), in the barrier region was observed in 64- and 70-year-old lenses but not in a 23-year-old lens. An increase in the dihydroceramide, DHCer (d18:0/16:0), in the lens nucleus was also observed in the older lenses. These findings were supported by ESI mass spectrometry analysis of lipid extracts from lenses dissected into outer, barrier, and nuclear regions. A subsequent analysis of 18 lenses ages 20–72 years revealed that sphingomyelin levels increased with age in the barrier region until reaching a plateau at approximately 40 years of age. Such changes in lipid composition will have a significant impact on the physical properties of the fiber cell membranes and may be associated with the formation of a barrier.http://www.sciencedirect.com/science/article/pii/S0022227520423053cataractMALDI imagingsphingomyelindihydrosphingomyelinphospholipidceramide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jane M. Deeley Joseph A. Hankin Michael G. Friedrich Robert C. Murphy Roger J.W. Truscott Todd W. Mitchell Stephen J. Blanksby |
spellingShingle |
Jane M. Deeley Joseph A. Hankin Michael G. Friedrich Robert C. Murphy Roger J.W. Truscott Todd W. Mitchell Stephen J. Blanksby Sphingolipid distribution changes with age in the human lens[S] Journal of Lipid Research cataract MALDI imaging sphingomyelin dihydrosphingomyelin phospholipid ceramide |
author_facet |
Jane M. Deeley Joseph A. Hankin Michael G. Friedrich Robert C. Murphy Roger J.W. Truscott Todd W. Mitchell Stephen J. Blanksby |
author_sort |
Jane M. Deeley |
title |
Sphingolipid distribution changes with age in the human lens[S] |
title_short |
Sphingolipid distribution changes with age in the human lens[S] |
title_full |
Sphingolipid distribution changes with age in the human lens[S] |
title_fullStr |
Sphingolipid distribution changes with age in the human lens[S] |
title_full_unstemmed |
Sphingolipid distribution changes with age in the human lens[S] |
title_sort |
sphingolipid distribution changes with age in the human lens[s] |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2010-09-01 |
description |
The formation of an internal barrier to the diffusion of small molecules in the lens during middle age is hypothesized to be a key event in the development of age-related nuclear (ARN) cataract. Changes in membrane lipids with age may be responsible. In this study, we investigated the effect of age on the distribution of sphingomyelins, the most abundant lens phospholipids. Human lens sections were initially analyzed by MALDI mass spectrometry imaging. A distinct annular distribution of the dihydrosphingomyelin, DHSM (d18:0/16:0), in the barrier region was observed in 64- and 70-year-old lenses but not in a 23-year-old lens. An increase in the dihydroceramide, DHCer (d18:0/16:0), in the lens nucleus was also observed in the older lenses. These findings were supported by ESI mass spectrometry analysis of lipid extracts from lenses dissected into outer, barrier, and nuclear regions. A subsequent analysis of 18 lenses ages 20–72 years revealed that sphingomyelin levels increased with age in the barrier region until reaching a plateau at approximately 40 years of age. Such changes in lipid composition will have a significant impact on the physical properties of the fiber cell membranes and may be associated with the formation of a barrier. |
topic |
cataract MALDI imaging sphingomyelin dihydrosphingomyelin phospholipid ceramide |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520423053 |
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