How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits
Phenolic substances affect the quality of beer and some of them have the health benefit for the consumer. Their content in the final product is influenced by a number of raw material-technological factors. We carried out 200 L pilot brews of pale lager with the different maturation period and simila...
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Research Institute of Brewing and Malting, Plc.
2019-07-01
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doaj-0e22bb3289dc4ec4a2eb4a2f4d558ac42020-11-24T21:30:42ZcesResearch Institute of Brewing and Malting, Plc.Kvasný průmysl2570-86192019-07-01654120–126120–12610.18832/kp2019.65.120182How does fermentation, filtration and stabilization of beer affect polyphenols with health benefitsAlexandr Mikyška0Martin Dušek1Martin Slabý2Research Institute of Brewing and Malting, plc., Lípová 15, CZ 120 44 Praha 2Research Institute of Brewing and Malting, plc., Lípová 15, CZ 120 44 Praha 2Research Institute of Brewing and Malting, plc., Lípová 15, CZ 120 44 Praha 2Phenolic substances affect the quality of beer and some of them have the health benefit for the consumer. Their content in the final product is influenced by a number of raw material-technological factors. We carried out 200 L pilot brews of pale lager with the different maturation period and similar brews focused on the effect of filter material (depth filtration plates /DFP/ and polyester sulfone membrane /PES/) and beer stabilization with protein and polyphenol sorbents. Flavonoids were determined using liquid chromatography in conjunction with high resolution mass spectrometry (LC-HR/ MS) and sample preparation by QuEChERS method. Additionally, total polyphenols and anthocyanogens was measured. The major reduction of both total polyphenols and flavonoids occurred in the first 2–3 weeks of maturation, the longer maturation had no impact on the loss of polyphenols. Filtration of beer with DFP significantly reduced the amount of anthocyanogens, but had no effect on monomeric flavonoid polyphenols. This technique could improve colloidal stability while preserving flavonoids. Conversely, PES membrane filtering greatly reduced the prenylflavonoid content (by 85%), and reduced the amount of flavanols and flavonols at levels comparable to those of PVPP-based polyphenol sorbent (25–35%). Flavonoids in beer could be largely influenced not only by PVPP treatment, but also by membrane filters used for both cold sterilization and primary beer filtration. Protein sorbent stabilization did not affect the content of flavonoids in beer. Decreases in flavonoid glycosides during filtration/stabilization were always lower than those of free flavonoids.http://www.kvasnyprumysl.eu/index.php/kp/article/view/182polyphenolsfiltrationcolloidal stabilizationbeerflavonoids |
collection |
DOAJ |
language |
ces |
format |
Article |
sources |
DOAJ |
author |
Alexandr Mikyška Martin Dušek Martin Slabý |
spellingShingle |
Alexandr Mikyška Martin Dušek Martin Slabý How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits Kvasný průmysl polyphenols filtration colloidal stabilization beer flavonoids |
author_facet |
Alexandr Mikyška Martin Dušek Martin Slabý |
author_sort |
Alexandr Mikyška |
title |
How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits |
title_short |
How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits |
title_full |
How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits |
title_fullStr |
How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits |
title_full_unstemmed |
How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits |
title_sort |
how does fermentation, filtration and stabilization of beer affect polyphenols with health benefits |
publisher |
Research Institute of Brewing and Malting, Plc. |
series |
Kvasný průmysl |
issn |
2570-8619 |
publishDate |
2019-07-01 |
description |
Phenolic substances affect the quality of beer and some of them have the health benefit for the consumer. Their content in the final product is influenced by a number of raw material-technological factors. We carried out 200 L pilot brews of pale lager with the different maturation period and similar brews focused on the effect of filter material (depth filtration plates /DFP/ and polyester sulfone membrane /PES/) and beer stabilization with protein and polyphenol sorbents. Flavonoids were determined using liquid chromatography in conjunction with high resolution mass spectrometry (LC-HR/ MS) and sample preparation by QuEChERS method. Additionally, total polyphenols and anthocyanogens was measured. The major reduction of both total polyphenols and flavonoids occurred in the first 2–3 weeks of maturation, the longer maturation had no impact on the loss of polyphenols. Filtration of beer with DFP significantly reduced the amount of anthocyanogens, but had no effect on monomeric flavonoid polyphenols. This technique could improve colloidal stability while preserving flavonoids. Conversely, PES membrane filtering greatly reduced the prenylflavonoid content (by 85%), and reduced the amount of flavanols and flavonols at levels comparable to those of PVPP-based polyphenol sorbent (25–35%). Flavonoids in beer could be largely influenced not only by PVPP treatment, but also by membrane filters used for both cold sterilization and primary beer filtration. Protein sorbent stabilization did not affect the content of flavonoids in beer. Decreases in flavonoid glycosides during filtration/stabilization were always lower than those of free flavonoids. |
topic |
polyphenols filtration colloidal stabilization beer flavonoids |
url |
http://www.kvasnyprumysl.eu/index.php/kp/article/view/182 |
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