Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk

Summary Beneficial biofilms may confer effective adaptation to food matrices that assist bacteria in enduring hostile environmental conditions. The matrices, for instance, dietary fibres of various food products, might serve as a natural scaffold for bacterial cells to adhere and grow as biofilms. H...

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Main Authors:	Satish Kumar Rajasekharan, Tali Paz‐Aviram, Shmuel Galili, Zipi Berkovich, Ram Reifen, Moshe Shemesh
Format:	Article
Language:	English
Published:	Wiley 2021-07-01
Series:	Microbial Biotechnology
Online Access:	https://doi.org/10.1111/1751-7915.13665

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spelling	doaj-0e2f5f4f0b97412b9a4b256a0890607c2021-07-26T21:47:24ZengWileyMicrobial Biotechnology1751-79152021-07-011441839184610.1111/1751-7915.13665Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milkSatish Kumar Rajasekharan0Tali Paz‐Aviram1Shmuel Galili2Zipi Berkovich3Ram Reifen4Moshe Shemesh5Departmet of Food Science Institute of Postharvvest Technology and Food Sciences Agricultural Research Organization (ARO) The Volcani Center Rishon LeZion 7528809 IsraelDepartmet of Food Science Institute of Postharvvest Technology and Food Sciences Agricultural Research Organization (ARO) The Volcani Center Rishon LeZion 7528809 IsraelDepartment of Vegetable and Field Crops Institute of Plant Sciences Agricultural Research Organization (ARO) The Volcani Center Rishon LeZion 7528809 IsraelInstitute of Biochemistry, Food Science and Nutrition The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot IsraelInstitute of Biochemistry, Food Science and Nutrition The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot IsraelDepartmet of Food Science Institute of Postharvvest Technology and Food Sciences Agricultural Research Organization (ARO) The Volcani Center Rishon LeZion 7528809 IsraelSummary Beneficial biofilms may confer effective adaptation to food matrices that assist bacteria in enduring hostile environmental conditions. The matrices, for instance, dietary fibres of various food products, might serve as a natural scaffold for bacterial cells to adhere and grow as biofilms. Here, we report on a unique interaction of Bacillus subtilis cells with the resistant starch fibresof chickpea milk (CPM), herein CPM fibres, along with the production of a reddish‐pink pigment. Genetic analysis identified the pigment as pulcherrimin, and also revealed the involvement of Spo0A/SinI pathway in modulating the observed phenotypes. Besides, through successful colonization of the CPM fibres, the wild‐type cells of B. subtilis displayed enhanced survivability and resilience to environmental stress, such as heat and in vitro gastrointestinal treatments. In total, we infer that the biofilm formation on CPM fibres is an adaptation response of B. subtilis for strategic survival.https://doi.org/10.1111/1751-7915.13665
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Satish Kumar Rajasekharan Tali Paz‐Aviram Shmuel Galili Zipi Berkovich Ram Reifen Moshe Shemesh
spellingShingle	Satish Kumar Rajasekharan Tali Paz‐Aviram Shmuel Galili Zipi Berkovich Ram Reifen Moshe Shemesh Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk Microbial Biotechnology
author_facet	Satish Kumar Rajasekharan Tali Paz‐Aviram Shmuel Galili Zipi Berkovich Ram Reifen Moshe Shemesh
author_sort	Satish Kumar Rajasekharan
title	Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk
title_short	Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk
title_full	Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk
title_fullStr	Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk
title_full_unstemmed	Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk
title_sort	biofilm formation onto starch fibres by bacillus subtilis governs its successful adaptation to chickpea milk
publisher	Wiley
series	Microbial Biotechnology
issn	1751-7915
publishDate	2021-07-01
description	Summary Beneficial biofilms may confer effective adaptation to food matrices that assist bacteria in enduring hostile environmental conditions. The matrices, for instance, dietary fibres of various food products, might serve as a natural scaffold for bacterial cells to adhere and grow as biofilms. Here, we report on a unique interaction of Bacillus subtilis cells with the resistant starch fibresof chickpea milk (CPM), herein CPM fibres, along with the production of a reddish‐pink pigment. Genetic analysis identified the pigment as pulcherrimin, and also revealed the involvement of Spo0A/SinI pathway in modulating the observed phenotypes. Besides, through successful colonization of the CPM fibres, the wild‐type cells of B. subtilis displayed enhanced survivability and resilience to environmental stress, such as heat and in vitro gastrointestinal treatments. In total, we infer that the biofilm formation on CPM fibres is an adaptation response of B. subtilis for strategic survival.
url	https://doi.org/10.1111/1751-7915.13665
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Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk

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