Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal Donor
Altered intestinal microbiota is associated with systemic and intestinal diseases, such as inflammatory bowel disease (IBD). Dysbiotic microbiota with enhanced proinflammatory capacity is characterized by depletion of anaerobic commensals, increased proportion of facultatively anaerobic bacteria, as...
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doaj-0e3cb4e4b6c344379c742c279af36e072020-11-25T02:28:44ZengMDPI AGNutrients2072-66432020-03-011293593510.3390/nu12040935Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal DonorKaisa Hiippala0Veera Kainulainen1Maiju Suutarinen2Tuomas Heini3Jolene R. Bowers4Daniel Jasso-Selles5Darrin Lemmer6Michael Valentine7Riley Barnes8David M. Engelthaler9Reetta Satokari10Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00290 Helsinki, FinlandHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00290 Helsinki, FinlandHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00290 Helsinki, FinlandHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00290 Helsinki, FinlandTranslational Genomics Research Institute, Pathogen and Microbiome Division, Flagstaff, AZ 86001, USATranslational Genomics Research Institute, Pathogen and Microbiome Division, Flagstaff, AZ 86001, USATranslational Genomics Research Institute, Pathogen and Microbiome Division, Flagstaff, AZ 86001, USATranslational Genomics Research Institute, Pathogen and Microbiome Division, Flagstaff, AZ 86001, USATranslational Genomics Research Institute, Pathogen and Microbiome Division, Flagstaff, AZ 86001, USATranslational Genomics Research Institute, Pathogen and Microbiome Division, Flagstaff, AZ 86001, USAHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00290 Helsinki, FinlandAltered intestinal microbiota is associated with systemic and intestinal diseases, such as inflammatory bowel disease (IBD). Dysbiotic microbiota with enhanced proinflammatory capacity is characterized by depletion of anaerobic commensals, increased proportion of facultatively anaerobic bacteria, as well as reduced diversity and stability. In this study, we developed a high-throughput in vitro screening assay to isolate intestinal commensal bacteria with anti-inflammatory capacity from a healthy fecal microbiota transplantation donor. Freshly isolated gut bacteria were screened for their capacity to attenuate <i>Escherichia coli</i> lipopolysaccharide (LPS)-induced interleukin 8 (IL-8) release from HT-29 cells. The screen yielded a number of <i>Bacteroides</i> and <i>Parabacteroides</i> isolates, which were identified as <i>P. distasonis</i>, <i>B. caccae</i>, <i>B. intestinalis</i>, <i>B. uniformis</i>, <i>B. fragilis</i>, <i>B. vulgatus</i> and <i>B. ovatus</i> using whole genome sequencing. We observed that a cell-cell contact with the epithelium was not necessary to alleviate in vitro inflammation as spent culture media from the isolates were also effective and the anti-inflammatory action did not correlate with the enterocyte adherence capacity of the isolates. The anti-inflammatory isolates also exerted enterocyte monolayer reinforcing action and lacked essential genes to synthetize hexa-acylated, proinflammatory lipid A, part of LPS. Yet, the anti-inflammatory effector molecules remain to be identified. The <i>Bacteroides</i> strains isolated and characterized in this study have potential to be used as so-called next-generation probiotics.https://www.mdpi.com/2072-6643/12/4/935bacteroidesgut homeostasishost-microbe interactionsimmunomodulationLPSnext-generation probiotics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kaisa Hiippala Veera Kainulainen Maiju Suutarinen Tuomas Heini Jolene R. Bowers Daniel Jasso-Selles Darrin Lemmer Michael Valentine Riley Barnes David M. Engelthaler Reetta Satokari |
spellingShingle |
Kaisa Hiippala Veera Kainulainen Maiju Suutarinen Tuomas Heini Jolene R. Bowers Daniel Jasso-Selles Darrin Lemmer Michael Valentine Riley Barnes David M. Engelthaler Reetta Satokari Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal Donor Nutrients bacteroides gut homeostasis host-microbe interactions immunomodulation LPS next-generation probiotics |
author_facet |
Kaisa Hiippala Veera Kainulainen Maiju Suutarinen Tuomas Heini Jolene R. Bowers Daniel Jasso-Selles Darrin Lemmer Michael Valentine Riley Barnes David M. Engelthaler Reetta Satokari |
author_sort |
Kaisa Hiippala |
title |
Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal Donor |
title_short |
Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal Donor |
title_full |
Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal Donor |
title_fullStr |
Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal Donor |
title_full_unstemmed |
Isolation of Anti-Inflammatory and Epithelium Reinforcing <i>Bacteroides</i> and <i>Parabacteroides</i> Spp. from A Healthy Fecal Donor |
title_sort |
isolation of anti-inflammatory and epithelium reinforcing <i>bacteroides</i> and <i>parabacteroides</i> spp. from a healthy fecal donor |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2020-03-01 |
description |
Altered intestinal microbiota is associated with systemic and intestinal diseases, such as inflammatory bowel disease (IBD). Dysbiotic microbiota with enhanced proinflammatory capacity is characterized by depletion of anaerobic commensals, increased proportion of facultatively anaerobic bacteria, as well as reduced diversity and stability. In this study, we developed a high-throughput in vitro screening assay to isolate intestinal commensal bacteria with anti-inflammatory capacity from a healthy fecal microbiota transplantation donor. Freshly isolated gut bacteria were screened for their capacity to attenuate <i>Escherichia coli</i> lipopolysaccharide (LPS)-induced interleukin 8 (IL-8) release from HT-29 cells. The screen yielded a number of <i>Bacteroides</i> and <i>Parabacteroides</i> isolates, which were identified as <i>P. distasonis</i>, <i>B. caccae</i>, <i>B. intestinalis</i>, <i>B. uniformis</i>, <i>B. fragilis</i>, <i>B. vulgatus</i> and <i>B. ovatus</i> using whole genome sequencing. We observed that a cell-cell contact with the epithelium was not necessary to alleviate in vitro inflammation as spent culture media from the isolates were also effective and the anti-inflammatory action did not correlate with the enterocyte adherence capacity of the isolates. The anti-inflammatory isolates also exerted enterocyte monolayer reinforcing action and lacked essential genes to synthetize hexa-acylated, proinflammatory lipid A, part of LPS. Yet, the anti-inflammatory effector molecules remain to be identified. The <i>Bacteroides</i> strains isolated and characterized in this study have potential to be used as so-called next-generation probiotics. |
topic |
bacteroides gut homeostasis host-microbe interactions immunomodulation LPS next-generation probiotics |
url |
https://www.mdpi.com/2072-6643/12/4/935 |
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