An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of Vasospasm
Objective: Vasospasm is a severe complication in patients with aneurysmal subarachnoid hemorrhage (aSAH) and cannot be reliably predicted. Its pathophysiology remains elusive with the current body of evidence suggesting inflammation as one of the main driving forces. We here aimed to analyze circula...
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doaj-0e4f21a2e4a64c95afff5c476779e1ba2020-12-21T06:58:18ZengFrontiers Media S.A.Frontiers in Neurology1664-22952020-12-011110.3389/fneur.2020.587039587039An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of VasospasmSusanna Bacigaluppi0Susanna Bacigaluppi1Susanna Bacigaluppi2Federico Ivaldi3Nicola L. Bragazzi4Federica Benvenuto5Fabio Gallo6Alessandro D'Andrea7Paolo Severi8Antonio Uccelli9Antonio Uccelli10Gianluigi Zona11Gianluigi Zona12Department of Neurosurgery-IRCCS Ospedale Policlinico San Martino, Genoa, ItalyDINOGMI & CEBR, University of Genoa, Genoa, ItalyDepartment of Neurosurgery-E.O. Ospedali Galliera, Genoa, ItalyDINOGMI & CEBR, University of Genoa, Genoa, ItalyDISSAL Department of Health Sciences, Università di Genova, Genoa, ItalyDINOGMI & CEBR, University of Genoa, Genoa, ItalyDISSAL Department of Health Sciences, Università di Genova, Genoa, ItalyDepartment of Neurosurgery-IRCCS Ospedale Policlinico San Martino, Genoa, ItalyDepartment of Neurosurgery-E.O. Ospedali Galliera, Genoa, ItalyDepartment of Neurosurgery-IRCCS Ospedale Policlinico San Martino, Genoa, ItalyDINOGMI & CEBR, University of Genoa, Genoa, ItalyDepartment of Neurosurgery-IRCCS Ospedale Policlinico San Martino, Genoa, ItalyDINOGMI & CEBR, University of Genoa, Genoa, ItalyObjective: Vasospasm is a severe complication in patients with aneurysmal subarachnoid hemorrhage (aSAH) and cannot be reliably predicted. Its pathophysiology remains elusive with the current body of evidence suggesting inflammation as one of the main driving forces. We here aimed to analyze circulating immune cell subsets over time in patients with aSAH with or without vasospasm.Methods: We performed a prospective observational study recruiting patients with spontaneous aSAH. Peripheral blood withdrawn at pre-specified time-points after aSAH, day 0, days 3–4, 6–8, 10–11, 13–15, and 18–21. Flow cytometry analysis, cell blood counts, and laboratory and diagnostic parameters were performed. Patients were monitored by transcranial Doppler for vasospasm as well as by advanced imaging and divided into a group with (VS) and without vasospasm VS (NVS).Results: We included 42 patients for study analysis, 21 VS and 21 NVS. An early significant increase at day 0 in platelet, leukocyte, neutrophil, lymphocyte, NK lymphocyte, monocyte, and CD 14++ CD16− DR+ monocyte counts was found in patients with later ensuing vasospasm. The early differences in platelets, leukocytes, lymphocytes, and NK lymphocytes remained significant on multivariate analysis.Conclusions: An early increase of immune cellular subsets in aSAH may contribute to predict VS.https://www.frontiersin.org/articles/10.3389/fneur.2020.587039/fullaneurysmal subarachnoid hemorrhageinflammatory responseleukocytessystemic inflammationimmune responsevasospasm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Susanna Bacigaluppi Susanna Bacigaluppi Susanna Bacigaluppi Federico Ivaldi Nicola L. Bragazzi Federica Benvenuto Fabio Gallo Alessandro D'Andrea Paolo Severi Antonio Uccelli Antonio Uccelli Gianluigi Zona Gianluigi Zona |
spellingShingle |
Susanna Bacigaluppi Susanna Bacigaluppi Susanna Bacigaluppi Federico Ivaldi Nicola L. Bragazzi Federica Benvenuto Fabio Gallo Alessandro D'Andrea Paolo Severi Antonio Uccelli Antonio Uccelli Gianluigi Zona Gianluigi Zona An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of Vasospasm Frontiers in Neurology aneurysmal subarachnoid hemorrhage inflammatory response leukocytes systemic inflammation immune response vasospasm |
author_facet |
Susanna Bacigaluppi Susanna Bacigaluppi Susanna Bacigaluppi Federico Ivaldi Nicola L. Bragazzi Federica Benvenuto Fabio Gallo Alessandro D'Andrea Paolo Severi Antonio Uccelli Antonio Uccelli Gianluigi Zona Gianluigi Zona |
author_sort |
Susanna Bacigaluppi |
title |
An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of Vasospasm |
title_short |
An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of Vasospasm |
title_full |
An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of Vasospasm |
title_fullStr |
An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of Vasospasm |
title_full_unstemmed |
An Early Increase of Blood Leukocyte Subsets in Aneurysmal Subarachnoid Hemorrhage Is Predictive of Vasospasm |
title_sort |
early increase of blood leukocyte subsets in aneurysmal subarachnoid hemorrhage is predictive of vasospasm |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neurology |
issn |
1664-2295 |
publishDate |
2020-12-01 |
description |
Objective: Vasospasm is a severe complication in patients with aneurysmal subarachnoid hemorrhage (aSAH) and cannot be reliably predicted. Its pathophysiology remains elusive with the current body of evidence suggesting inflammation as one of the main driving forces. We here aimed to analyze circulating immune cell subsets over time in patients with aSAH with or without vasospasm.Methods: We performed a prospective observational study recruiting patients with spontaneous aSAH. Peripheral blood withdrawn at pre-specified time-points after aSAH, day 0, days 3–4, 6–8, 10–11, 13–15, and 18–21. Flow cytometry analysis, cell blood counts, and laboratory and diagnostic parameters were performed. Patients were monitored by transcranial Doppler for vasospasm as well as by advanced imaging and divided into a group with (VS) and without vasospasm VS (NVS).Results: We included 42 patients for study analysis, 21 VS and 21 NVS. An early significant increase at day 0 in platelet, leukocyte, neutrophil, lymphocyte, NK lymphocyte, monocyte, and CD 14++ CD16− DR+ monocyte counts was found in patients with later ensuing vasospasm. The early differences in platelets, leukocytes, lymphocytes, and NK lymphocytes remained significant on multivariate analysis.Conclusions: An early increase of immune cellular subsets in aSAH may contribute to predict VS. |
topic |
aneurysmal subarachnoid hemorrhage inflammatory response leukocytes systemic inflammation immune response vasospasm |
url |
https://www.frontiersin.org/articles/10.3389/fneur.2020.587039/full |
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