Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China
Cryptosporidium spp. and Enterocytozoon bieneusi are two prevalent opportunistic pathogens in humans and animals. Currently, few data are available on genetic characterization of both pathogens in rabbits in China. The aim of the present study was to understand prevalence and genetic characterizatio...
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doaj-0e6b1290f5b34908affb4fbf81f97d102021-02-02T08:03:13ZengEDP SciencesParasite1776-10422016-01-01235210.1051/parasite/2016063parasite160061Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, ChinaYang ZiyinZhao WeiShen YujuanZhang WeizheShi YingRen GuangxuYang DiLing HongYang FengkunLiu AiqinCao JianpingCryptosporidium spp. and Enterocytozoon bieneusi are two prevalent opportunistic pathogens in humans and animals. Currently, few data are available on genetic characterization of both pathogens in rabbits in China. The aim of the present study was to understand prevalence and genetic characterization of Cryptosporidium spp. and E. bieneusi in rabbits. We collected 215 fecal samples from 150 Rex rabbits and 65 New Zealand White rabbits on two different farms in Heilongjiang Province, China. Cryptosporidium spp. and E. bieneusi were tested by polymerase chain reaction (PCR) and sequencing the partial small subunit of ribosomal DNA (SSU rDNA) and the internal transcribed spacer (ITS) region of rDNA, respectively. Cryptosporidium was detected in 3.3% (5/150) of Rex rabbits and 29.2% (19/65) of New Zealand White rabbits. All the 24 Cryptosporidium isolates were identified as C. cuniculus. Enterocytozoon bieneusi was only found in 14.7% (22/150) of Rex rabbits. Five known genotypes: CHN-RD1 (n = 12), D (n = 3), Type IV (n = 2), Peru6 (n = 1), and I (n = 1), and three novel ones CHN-RR1 to CHN-RR3 (one each) were detected. By analyzing the 60-kDa glycoprotein (gp60) gene sequences of C. cuniculus isolates, three subtypes were obtained: VbA28 (n = 2), VbA29 (n = 16), and VbA32 (n = 3). All these three C. cuniculus subtypes were reported previously in humans. Four known E. bieneusi genotypes have been found to be present in humans. The three novel ones fell into zoonotic group 1. The results suggest zoonotic potential of C. cuniculus and E. bieneusi isolates in rabbits.http://dx.doi.org/10.1051/parasite/2016063rabbitsCryptosporidiumEnterocytozoon bieneusiITS regionSSU rDNAgp60 gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yang Ziyin Zhao Wei Shen Yujuan Zhang Weizhe Shi Ying Ren Guangxu Yang Di Ling Hong Yang Fengkun Liu Aiqin Cao Jianping |
spellingShingle |
Yang Ziyin Zhao Wei Shen Yujuan Zhang Weizhe Shi Ying Ren Guangxu Yang Di Ling Hong Yang Fengkun Liu Aiqin Cao Jianping Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China Parasite rabbits Cryptosporidium Enterocytozoon bieneusi ITS region SSU rDNA gp60 gene |
author_facet |
Yang Ziyin Zhao Wei Shen Yujuan Zhang Weizhe Shi Ying Ren Guangxu Yang Di Ling Hong Yang Fengkun Liu Aiqin Cao Jianping |
author_sort |
Yang Ziyin |
title |
Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China |
title_short |
Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China |
title_full |
Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China |
title_fullStr |
Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China |
title_full_unstemmed |
Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China |
title_sort |
subtyping of cryptosporidium cuniculus and genotyping of enterocytozoon bieneusi in rabbits in two farms in heilongjiang province, china |
publisher |
EDP Sciences |
series |
Parasite |
issn |
1776-1042 |
publishDate |
2016-01-01 |
description |
Cryptosporidium spp. and Enterocytozoon bieneusi are two prevalent opportunistic pathogens in humans and animals. Currently, few data are available on genetic characterization of both pathogens in rabbits in China. The aim of the present study was to understand prevalence and genetic characterization of Cryptosporidium spp. and E. bieneusi in rabbits. We collected 215 fecal samples from 150 Rex rabbits and 65 New Zealand White rabbits on two different farms in Heilongjiang Province, China. Cryptosporidium spp. and E. bieneusi were tested by polymerase chain reaction (PCR) and sequencing the partial small subunit of ribosomal DNA (SSU rDNA) and the internal transcribed spacer (ITS) region of rDNA, respectively. Cryptosporidium was detected in 3.3% (5/150) of Rex rabbits and 29.2% (19/65) of New Zealand White rabbits. All the 24 Cryptosporidium isolates were identified as C. cuniculus. Enterocytozoon bieneusi was only found in 14.7% (22/150) of Rex rabbits. Five known genotypes: CHN-RD1 (n = 12), D (n = 3), Type IV (n = 2), Peru6 (n = 1), and I (n = 1), and three novel ones CHN-RR1 to CHN-RR3 (one each) were detected. By analyzing the 60-kDa glycoprotein (gp60) gene sequences of C. cuniculus isolates, three subtypes were obtained: VbA28 (n = 2), VbA29 (n = 16), and VbA32 (n = 3). All these three C. cuniculus subtypes were reported previously in humans. Four known E. bieneusi genotypes have been found to be present in humans. The three novel ones fell into zoonotic group 1. The results suggest zoonotic potential of C. cuniculus and E. bieneusi isolates in rabbits. |
topic |
rabbits Cryptosporidium Enterocytozoon bieneusi ITS region SSU rDNA gp60 gene |
url |
http://dx.doi.org/10.1051/parasite/2016063 |
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