Template preparation for rapid PCR in Colletotrichum lindemuthianum

Template preparation for rapid PCR in Colletotrichum lindemuthianum

Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic...

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Bibliographic Details
Main Authors: Roca M. Gabriela, Davide Lisete C., Wheals Alan E.
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2003-01-01
Series:Brazilian Journal of Microbiology
Subjects:
anthracnose
hygromycin resistance
internal transcribed spacer
rapid screening
PCR methodology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003
Description
Summary:Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).
ISSN:1517-8382
1678-4405

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