Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood
<p>Abstract</p> <p>This study evaluated the efficiency of pediatric mid-turbinate nasal flocked swabs used by parents in 203 children aged 6 months to 5 years with signs and symptoms of respiratory disease. Two nasal samples were collected from each child in a randomised sequence:...
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doaj-0e88f0e31b4e44869de348cd142964212020-11-24T23:28:38ZengBMCVirology Journal1743-422X2010-04-01718510.1186/1743-422X-7-85Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhoodFossali EmilioMilani GregorioGaleone CarlottaTagliabue ClaudiaValzano AntoniaDaleno CristinaMolteni Claudio GEsposito SusannaMarchisio PaolaPrincipi Nicola<p>Abstract</p> <p>This study evaluated the efficiency of pediatric mid-turbinate nasal flocked swabs used by parents in 203 children aged 6 months to 5 years with signs and symptoms of respiratory disease. Two nasal samples were collected from each child in a randomised sequence: one by a trained pediatrician and one by a parent. The real-time polymerase chain reaction influenza virus detection rates were similar in the samples collected using the two methods (Cohen's kappa = 0.86), as were the cycle threshold values. In comparison with the pediatrician-collected samples, the sensitivity and specificity of the parental collections were respectively 89.3% (95% confidence interval [CI]: 77.8-100%) and 97.7% (95% CI: 95.5-100%), and the positive and negative predictive values were respectively 86.2% (95% CI: 73.7-95.1%) and 98.2% (95% CI: 96.4-100%). The children were significantly more satisfied with the parental collections (median values ± standard deviation, 1.59 ± 0.55 <it>vs </it>3.51 ± 0.36; p < 0.0001). These findings show that mid-turbinate nasal flocked swabs specifically designed for infants and children can be used by parents without reducing the influenza virus detection rate. Moreover, the direct involvement of parents significantly increases patient acceptance, thus simplifying collection and suggesting that this novel swab design should be considered for epidemiological surveys and vaccine efficacy studies.</p> http://www.virologyj.com/content/7/1/85 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fossali Emilio Milani Gregorio Galeone Carlotta Tagliabue Claudia Valzano Antonia Daleno Cristina Molteni Claudio G Esposito Susanna Marchisio Paola Principi Nicola |
spellingShingle |
Fossali Emilio Milani Gregorio Galeone Carlotta Tagliabue Claudia Valzano Antonia Daleno Cristina Molteni Claudio G Esposito Susanna Marchisio Paola Principi Nicola Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood Virology Journal |
author_facet |
Fossali Emilio Milani Gregorio Galeone Carlotta Tagliabue Claudia Valzano Antonia Daleno Cristina Molteni Claudio G Esposito Susanna Marchisio Paola Principi Nicola |
author_sort |
Fossali Emilio |
title |
Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood |
title_short |
Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood |
title_full |
Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood |
title_fullStr |
Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood |
title_full_unstemmed |
Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood |
title_sort |
collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2010-04-01 |
description |
<p>Abstract</p> <p>This study evaluated the efficiency of pediatric mid-turbinate nasal flocked swabs used by parents in 203 children aged 6 months to 5 years with signs and symptoms of respiratory disease. Two nasal samples were collected from each child in a randomised sequence: one by a trained pediatrician and one by a parent. The real-time polymerase chain reaction influenza virus detection rates were similar in the samples collected using the two methods (Cohen's kappa = 0.86), as were the cycle threshold values. In comparison with the pediatrician-collected samples, the sensitivity and specificity of the parental collections were respectively 89.3% (95% confidence interval [CI]: 77.8-100%) and 97.7% (95% CI: 95.5-100%), and the positive and negative predictive values were respectively 86.2% (95% CI: 73.7-95.1%) and 98.2% (95% CI: 96.4-100%). The children were significantly more satisfied with the parental collections (median values ± standard deviation, 1.59 ± 0.55 <it>vs </it>3.51 ± 0.36; p < 0.0001). These findings show that mid-turbinate nasal flocked swabs specifically designed for infants and children can be used by parents without reducing the influenza virus detection rate. Moreover, the direct involvement of parents significantly increases patient acceptance, thus simplifying collection and suggesting that this novel swab design should be considered for epidemiological surveys and vaccine efficacy studies.</p> |
url |
http://www.virologyj.com/content/7/1/85 |
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