Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts
Herpes simplex virus type 1 (HSV-1) infection has a prevalence of 70% in the human population. Treatment is based on acyclovir, valacyclovir, and foscarnet, three drugs that share the same mechanism of action and of which resistant strains have been isolated from patients. In this aspect, innovative...
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doaj-0e8d16c4ba0348f1930ec3087b8a1d692020-11-24T21:28:58ZengHindawi LimitedThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/174837174837Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae ExtractsPaula Faral-Tello0Santiago Mirazo1Carmelo Dutra2Andrés Pérez3Lucía Geis-Asteggiante4Sandra Frabasile5Elina Koncke6Danilo Davyt7Lucía Cavallaro8Horacio Heinzen9Juan Arbiza10Sección Virología, Facultad de Ciencias, Universidad de la República, 11400 Montevideo, UruguaySección Virología, Facultad de Ciencias, Universidad de la República, 11400 Montevideo, UruguayCátedra de Productos Naturales Facultad de Química, Universidad de la República, 11800 Montevideo, UruguayCátedra de Productos Naturales Facultad de Química, Universidad de la República, 11800 Montevideo, UruguayCátedra de Productos Naturales Facultad de Química, Universidad de la República, 11800 Montevideo, UruguaySección Virología, Facultad de Ciencias, Universidad de la República, 11400 Montevideo, UruguaySección Virología, Facultad de Ciencias, Universidad de la República, 11400 Montevideo, UruguayCátedra de Productos Naturales Facultad de Química, Universidad de la República, 11800 Montevideo, UruguayCátedra de Virología, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, C1113AAD Buenos Aires, ArgentinaCátedra de Productos Naturales Facultad de Química, Universidad de la República, 11800 Montevideo, UruguaySección Virología, Facultad de Ciencias, Universidad de la República, 11400 Montevideo, UruguayHerpes simplex virus type 1 (HSV-1) infection has a prevalence of 70% in the human population. Treatment is based on acyclovir, valacyclovir, and foscarnet, three drugs that share the same mechanism of action and of which resistant strains have been isolated from patients. In this aspect, innovative drug therapies are required. Natural products offer unlimited opportunities for the discovery of antiviral compounds. In this study, 28 extracts corresponding to 24 plant species and 4 alga species were assayed in vitro to detect antiviral activity against HSV-1. Six of the methanolic extracts inactivated viral particles by direct interaction and 14 presented antiviral activity when incubated with cells already infected. Most interesting antiviral activity values obtained are those of Limonium brasiliense, Psidium guajava, and Phyllanthus niruri, which inhibit HSV-1 replication in vitro with 50% effective concentration (EC50) values of 185, 118, and 60 μg/mL, respectively. For these extracts toxicity values were calculated and therefore selectivity indexes (SI) obtained. Further characterization of the bioactive components of antiviral plants will pave the way for the discovery of new compounds against HSV-1.http://dx.doi.org/10.1100/2012/174837 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paula Faral-Tello Santiago Mirazo Carmelo Dutra Andrés Pérez Lucía Geis-Asteggiante Sandra Frabasile Elina Koncke Danilo Davyt Lucía Cavallaro Horacio Heinzen Juan Arbiza |
spellingShingle |
Paula Faral-Tello Santiago Mirazo Carmelo Dutra Andrés Pérez Lucía Geis-Asteggiante Sandra Frabasile Elina Koncke Danilo Davyt Lucía Cavallaro Horacio Heinzen Juan Arbiza Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts The Scientific World Journal |
author_facet |
Paula Faral-Tello Santiago Mirazo Carmelo Dutra Andrés Pérez Lucía Geis-Asteggiante Sandra Frabasile Elina Koncke Danilo Davyt Lucía Cavallaro Horacio Heinzen Juan Arbiza |
author_sort |
Paula Faral-Tello |
title |
Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts |
title_short |
Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts |
title_full |
Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts |
title_fullStr |
Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts |
title_full_unstemmed |
Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts |
title_sort |
cytotoxic, virucidal, and antiviral activity of south american plant and algae extracts |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2012-01-01 |
description |
Herpes simplex virus type 1 (HSV-1) infection has a prevalence of 70% in the human population. Treatment is based on acyclovir, valacyclovir, and foscarnet, three drugs that share the same mechanism of action and of which resistant strains have been isolated from patients. In this aspect, innovative drug therapies are required. Natural products offer unlimited opportunities for the discovery of antiviral compounds. In this study, 28 extracts corresponding to 24 plant species and 4 alga species were assayed in vitro to detect antiviral activity against HSV-1. Six of the methanolic extracts inactivated viral particles by direct interaction and 14 presented antiviral activity when incubated with cells already infected. Most interesting antiviral activity values obtained are those of Limonium brasiliense, Psidium guajava, and Phyllanthus niruri, which inhibit HSV-1 replication in vitro with 50% effective concentration (EC50) values of 185, 118, and 60 μg/mL, respectively. For these extracts toxicity values were calculated and therefore selectivity indexes (SI) obtained. Further characterization of the bioactive components of antiviral plants will pave the way for the discovery of new compounds against HSV-1. |
url |
http://dx.doi.org/10.1100/2012/174837 |
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