Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane
Enzymatic membrane bioreactors (EMBRs) possess the characteristic of combining catalysis with separation, and therefore have promising application potentials. In order to achieve a high-performance EMBR, membrane property, as well as operating parameters, should give special cause for concerns. In t...
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doaj-0ea808e07d644ae9af5ede2506dd56372020-11-25T01:16:08ZengMDPI AGCatalysts2073-43442019-05-019649510.3390/catal9060495catal9060495Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber MembranePeng-Cheng Chen0Zhen Ma1Xue-Yan Zhu2Da-Jing Chen3Xiao-Jun Huang4The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaSchool of medicine, Hangzhou Normal University, Hangzhou 311121, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaSchool of medicine, Hangzhou Normal University, Hangzhou 311121, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaEnzymatic membrane bioreactors (EMBRs) possess the characteristic of combining catalysis with separation, and therefore have promising application potentials. In order to achieve a high-performance EMBR, membrane property, as well as operating parameters, should give special cause for concerns. In this work, an EMBR based on hollow fiber polysulfone microfiltration membranes with radial gradient pore structure was fabricated and enzyme immobilization was achieved through pressure-driven filtration. Lipase from <i>Candida rugosa</i> was used for immobilization and EMBR performance was studied with the enzymatic hydrolysis of glycerol triacetate as a model reaction. The influences of membrane pore diameter, substrate feed direction as well as operational parameters of operation pressure, substrate concentration, and temperature on the EMBR activity were investigated with the production of hydrolysates kinetically fitted. The complete EMBR system showed the highest activity of 1.07 × 10<sup>4</sup> U⋅g<sup>−1</sup>. The results in this work indicate future efforts for improvement in EMBR.https://www.mdpi.com/2073-4344/9/6/495enzymatic membrane bioreactorgradient-pore membranelipasefiltrationhydrolysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peng-Cheng Chen Zhen Ma Xue-Yan Zhu Da-Jing Chen Xiao-Jun Huang |
spellingShingle |
Peng-Cheng Chen Zhen Ma Xue-Yan Zhu Da-Jing Chen Xiao-Jun Huang Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane Catalysts enzymatic membrane bioreactor gradient-pore membrane lipase filtration hydrolysis |
author_facet |
Peng-Cheng Chen Zhen Ma Xue-Yan Zhu Da-Jing Chen Xiao-Jun Huang |
author_sort |
Peng-Cheng Chen |
title |
Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane |
title_short |
Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane |
title_full |
Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane |
title_fullStr |
Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane |
title_full_unstemmed |
Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane |
title_sort |
fabrication and optimization of a lipase immobilized enzymatic membrane bioreactor based on polysulfone gradient-pore hollow fiber membrane |
publisher |
MDPI AG |
series |
Catalysts |
issn |
2073-4344 |
publishDate |
2019-05-01 |
description |
Enzymatic membrane bioreactors (EMBRs) possess the characteristic of combining catalysis with separation, and therefore have promising application potentials. In order to achieve a high-performance EMBR, membrane property, as well as operating parameters, should give special cause for concerns. In this work, an EMBR based on hollow fiber polysulfone microfiltration membranes with radial gradient pore structure was fabricated and enzyme immobilization was achieved through pressure-driven filtration. Lipase from <i>Candida rugosa</i> was used for immobilization and EMBR performance was studied with the enzymatic hydrolysis of glycerol triacetate as a model reaction. The influences of membrane pore diameter, substrate feed direction as well as operational parameters of operation pressure, substrate concentration, and temperature on the EMBR activity were investigated with the production of hydrolysates kinetically fitted. The complete EMBR system showed the highest activity of 1.07 × 10<sup>4</sup> U⋅g<sup>−1</sup>. The results in this work indicate future efforts for improvement in EMBR. |
topic |
enzymatic membrane bioreactor gradient-pore membrane lipase filtration hydrolysis |
url |
https://www.mdpi.com/2073-4344/9/6/495 |
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