Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the Plant
Bacillus firmus I-1582 is approved in Europe for the management of Meloidogyne on vegetable crops. However, little information about its modes of action and temperature requirements is available, despite the effect of these parameters in its efficacy. The cardinal temperatures for bacterial growth a...
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doaj-0ec256c30c214872b0f58c6da79aca412020-11-25T03:52:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-07-011110.3389/fpls.2020.00796515491Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the PlantZahra Ghahremani0Nuria Escudero1Daniel Beltrán-Anadón2Ester Saus3Ester Saus4Marina Cunquero5Jordi Andilla6Pablo Loza-Alvarez7Toni Gabaldón8Toni Gabaldón9Toni Gabaldón10F. Javier Sorribas11Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya, Barcelona, SpainDepartament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya, Barcelona, SpainDepartament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya, Barcelona, SpainBioinformatics and Genomics Program, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Barcelona, SpainDepartment of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, SpainInstitut de Ciències Fotòniques (ICFO), The Barcelona Institute of Science and Technology, Barcelona, SpainInstitut de Ciències Fotòniques (ICFO), The Barcelona Institute of Science and Technology, Barcelona, SpainInstitut de Ciències Fotòniques (ICFO), The Barcelona Institute of Science and Technology, Barcelona, SpainBioinformatics and Genomics Program, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Barcelona, SpainDepartment of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, SpainInstitució Catalana de Recerca i Estudis Avançats, Barcelona, SpainDepartament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya, Barcelona, SpainBacillus firmus I-1582 is approved in Europe for the management of Meloidogyne on vegetable crops. However, little information about its modes of action and temperature requirements is available, despite the effect of these parameters in its efficacy. The cardinal temperatures for bacterial growth and biofilm formation were determined. The bacteria was transformed with GFP to study its effect on nematode eggs and root colonization of tomato (Solanum lycopersicum) and cucumber (Cucumis sativus) by laser-scanning confocal microscopy. Induction of plant resistance was determined in split-root experiments and the dynamic regulation of genes related to jasmonic acid (JA) and salicylic acid (SA) by RT-qPCR at three different times after nematode inoculation. The bacteria was able to grow and form biofilms between 15 and 45°C; it degraded egg-shells and colonized eggs; it colonized tomato roots more extensively than cucumber roots; it induced systemic resistance in tomato, but not in cucumber; SA and JA related genes were primed at different times after nematode inoculation in tomato, but only the SA-related gene was up-regulated at 7 days after nematode inoculation in cucumber. In conclusion, B. firmus I-1582 is active at a wide range of temperatures; its optimal growth temperature is 35°C; it is able to degrade Meloidogyne eggs, and to colonize plant roots, inducing systemic resistance in a plant dependent species manner.https://www.frontiersin.org/article/10.3389/fpls.2020.00796/fullCucumis sativusinduced resistancenematode antagonistMeloidogyne incognitaroot-knot nematodesSolanum lycopersicum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zahra Ghahremani Nuria Escudero Daniel Beltrán-Anadón Ester Saus Ester Saus Marina Cunquero Jordi Andilla Pablo Loza-Alvarez Toni Gabaldón Toni Gabaldón Toni Gabaldón F. Javier Sorribas |
spellingShingle |
Zahra Ghahremani Nuria Escudero Daniel Beltrán-Anadón Ester Saus Ester Saus Marina Cunquero Jordi Andilla Pablo Loza-Alvarez Toni Gabaldón Toni Gabaldón Toni Gabaldón F. Javier Sorribas Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the Plant Frontiers in Plant Science Cucumis sativus induced resistance nematode antagonist Meloidogyne incognita root-knot nematodes Solanum lycopersicum |
author_facet |
Zahra Ghahremani Nuria Escudero Daniel Beltrán-Anadón Ester Saus Ester Saus Marina Cunquero Jordi Andilla Pablo Loza-Alvarez Toni Gabaldón Toni Gabaldón Toni Gabaldón F. Javier Sorribas |
author_sort |
Zahra Ghahremani |
title |
Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the Plant |
title_short |
Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the Plant |
title_full |
Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the Plant |
title_fullStr |
Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the Plant |
title_full_unstemmed |
Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and Through the Plant |
title_sort |
bacillus firmus strain i-1582, a nematode antagonist by itself and through the plant |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-07-01 |
description |
Bacillus firmus I-1582 is approved in Europe for the management of Meloidogyne on vegetable crops. However, little information about its modes of action and temperature requirements is available, despite the effect of these parameters in its efficacy. The cardinal temperatures for bacterial growth and biofilm formation were determined. The bacteria was transformed with GFP to study its effect on nematode eggs and root colonization of tomato (Solanum lycopersicum) and cucumber (Cucumis sativus) by laser-scanning confocal microscopy. Induction of plant resistance was determined in split-root experiments and the dynamic regulation of genes related to jasmonic acid (JA) and salicylic acid (SA) by RT-qPCR at three different times after nematode inoculation. The bacteria was able to grow and form biofilms between 15 and 45°C; it degraded egg-shells and colonized eggs; it colonized tomato roots more extensively than cucumber roots; it induced systemic resistance in tomato, but not in cucumber; SA and JA related genes were primed at different times after nematode inoculation in tomato, but only the SA-related gene was up-regulated at 7 days after nematode inoculation in cucumber. In conclusion, B. firmus I-1582 is active at a wide range of temperatures; its optimal growth temperature is 35°C; it is able to degrade Meloidogyne eggs, and to colonize plant roots, inducing systemic resistance in a plant dependent species manner. |
topic |
Cucumis sativus induced resistance nematode antagonist Meloidogyne incognita root-knot nematodes Solanum lycopersicum |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.00796/full |
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