SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, India
Objective:Zinc transporter 8 (ZnT8) is a multi-transmembrane protein situated in the insulin secretory granule of the islets of β-cells and is identified as a novel auto-antigen in type 1 diabetes (T1D). The gene coding for ZnT8, solute carrier family 30 member 8 (SLC30A8) is located on chromosome 8...
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Galenos Yayincilik
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doaj-0ee0fda43cfb4d8499caead75a15fdd32020-11-24T21:48:55ZengGalenos YayincilikJCRPE1308-57271308-57352019-03-01111556010.4274/jcrpe.galenos.2018.2018.019513049054SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, IndiaRamasamy Thirunavukkarasu0Arthur Joseph Asirvatham1Ayyappan Chitra2Mariakuttikan Jayalakshmi3 Madurai Kamaraj University, School of Biological Sciences, Department of Immunology, Madurai, India Government Rajaji Hospital, Clinic of Diabetology, Madurai, India Government Rajaji Hospital, Institute of Child Health and Research Centre, Madurai, India Madurai Kamaraj University, School of Biological Sciences, Department of Immunology, Madurai, India Objective:Zinc transporter 8 (ZnT8) is a multi-transmembrane protein situated in the insulin secretory granule of the islets of β-cells and is identified as a novel auto-antigen in type 1 diabetes (T1D). The gene coding for ZnT8, solute carrier family 30 member 8 (SLC30A8) is located on chromosome 8q24.11. This study aimed to identify the association of SLC30A8 rs13266634 C/T gene polymorphism with T1D in a sample of T1D children in Tamil Nadu, India.Methods:The family based study was conducted in 121 T1D patients and 214 of their family members as controls. The SLC30A8 gene rs13266634 C/T polymorphism was evaluated by polymerase chain reaction-restriction fragment length polymorphism.Results:No significant differences were observed in either allele (odds ratio: 0.92; confidence interval: 0.33-2.58; p=0.88) and genotype (CC: p=0.74; CT: p=0.82; TT: p=0.80) frequencies of rs13266634 C/T between T1D patients and controls. Transmission disequilibrium test has identified over-transmission of mutant T allele from parents to affected children (T: U=9:7) without statistical significance. Metaanalysis on the overall effects of rs13266634 C allele frequency was not different (p=0.10 and Pheterogeneity=0.99) in T1D patients as compared to the controls.Conclusion:The present study along with the meta-analysis does not show any substantial association of the rs13266634 C/T polymorphism with T1D development in this population. http://www.jcrpe.org/archives/archive-detail/article-preview/islc30a8-i-gene-rs13266634-c-t-polymorphism-in-chi/19598 Type 1 diabetesauto-antigenpolymorphismszinc transporter 8 autoantibodymeta-analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ramasamy Thirunavukkarasu Arthur Joseph Asirvatham Ayyappan Chitra Mariakuttikan Jayalakshmi |
spellingShingle |
Ramasamy Thirunavukkarasu Arthur Joseph Asirvatham Ayyappan Chitra Mariakuttikan Jayalakshmi SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, India JCRPE Type 1 diabetes auto-antigen polymorphisms zinc transporter 8 autoantibody meta-analysis |
author_facet |
Ramasamy Thirunavukkarasu Arthur Joseph Asirvatham Ayyappan Chitra Mariakuttikan Jayalakshmi |
author_sort |
Ramasamy Thirunavukkarasu |
title |
SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, India |
title_short |
SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, India |
title_full |
SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, India |
title_fullStr |
SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, India |
title_full_unstemmed |
SLC30A8 Gene rs13266634 C/T Polymorphism in Children with Type 1 Diabetes in Tamil Nadu, India |
title_sort |
slc30a8 gene rs13266634 c/t polymorphism in children with type 1 diabetes in tamil nadu, india |
publisher |
Galenos Yayincilik |
series |
JCRPE |
issn |
1308-5727 1308-5735 |
publishDate |
2019-03-01 |
description |
Objective:Zinc transporter 8 (ZnT8) is a multi-transmembrane protein situated in the insulin secretory granule of the islets of β-cells and is identified as a novel auto-antigen in type 1 diabetes (T1D). The gene coding for ZnT8, solute carrier family 30 member 8 (SLC30A8) is located on chromosome 8q24.11. This study aimed to identify the association of SLC30A8 rs13266634 C/T gene polymorphism with T1D in a sample of T1D children in Tamil Nadu, India.Methods:The family based study was conducted in 121 T1D patients and 214 of their family members as controls. The SLC30A8 gene rs13266634 C/T polymorphism was evaluated by polymerase chain reaction-restriction fragment length polymorphism.Results:No significant differences were observed in either allele (odds ratio: 0.92; confidence interval: 0.33-2.58; p=0.88) and genotype (CC: p=0.74; CT: p=0.82; TT: p=0.80) frequencies of rs13266634 C/T between T1D patients and controls. Transmission disequilibrium test has identified over-transmission of mutant T allele from parents to affected children (T: U=9:7) without statistical significance. Metaanalysis on the overall effects of rs13266634 C allele frequency was not different (p=0.10 and Pheterogeneity=0.99) in T1D patients as compared to the controls.Conclusion:The present study along with the meta-analysis does not show any substantial association of the rs13266634 C/T polymorphism with T1D development in this population. |
topic |
Type 1 diabetes auto-antigen polymorphisms zinc transporter 8 autoantibody meta-analysis |
url |
http://www.jcrpe.org/archives/archive-detail/article-preview/islc30a8-i-gene-rs13266634-c-t-polymorphism-in-chi/19598
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work_keys_str_mv |
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