Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided e...
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Universidade Estadual de Campinas
2015-03-01
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doaj-0ee83de14e544a32b734df068429d4632021-07-15T14:03:55ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252015-03-0114110.20396/bjos.v14i1.8640839Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyleMiriane Carneiro Machado Salgado0Adriana Mathias Pereira da Silva Marchin1Tábata de Mello Tera2Rosilene Fernandes da Rocha3Leonardo Marchini4Universidade Estadual Paulista – UNESPUniversidade Estadual Paulista – UNESPUniversidade Estadual Paulista – UNESPUniversidade Estadual Paulista – UNESPUniversity of IowaEstrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839MandibleMandibular condyleEthanolOvariectomy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miriane Carneiro Machado Salgado Adriana Mathias Pereira da Silva Marchin Tábata de Mello Tera Rosilene Fernandes da Rocha Leonardo Marchini |
spellingShingle |
Miriane Carneiro Machado Salgado Adriana Mathias Pereira da Silva Marchin Tábata de Mello Tera Rosilene Fernandes da Rocha Leonardo Marchini Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle Brazilian Journal of Oral Sciences Mandible Mandibular condyle Ethanol Ovariectomy |
author_facet |
Miriane Carneiro Machado Salgado Adriana Mathias Pereira da Silva Marchin Tábata de Mello Tera Rosilene Fernandes da Rocha Leonardo Marchini |
author_sort |
Miriane Carneiro Machado Salgado |
title |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_short |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_full |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_fullStr |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_full_unstemmed |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_sort |
effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
publisher |
Universidade Estadual de Campinas |
series |
Brazilian Journal of Oral Sciences |
issn |
1677-3225 |
publishDate |
2015-03-01 |
description |
Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups. |
topic |
Mandible Mandibular condyle Ethanol Ovariectomy |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839 |
work_keys_str_mv |
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1721300207913664512 |