Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats
Hydrogen sulfide (H2S) is a neuromodulator in the central nervous system. However, the physiological role of H2S in the nucleus ambiguus (NA) has rarely been reported. This research aimed to elucidate the role of H2S in the regulation of gastrointestinal motility in rats. Male Wistar rats were rando...
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doaj-0efd1f2a0edb455791d63cb2067211782020-11-25T03:48:06ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-09-011110.3389/fphys.2020.545184545184Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in RatsHongzhao Sun0Haikun Ding1Yuan Shi2Chenyu Li3Haoran Jin4Xiaoyue Yang5Zhaosong Chen6Pengpeng Tian7Jianping Zhu8Haiji Sun9School of Life Sciences, Qilu Normal University, Jinan, ChinaSchool of Life Sciences, Qilu Normal University, Jinan, ChinaSchool of Life Sciences, Qilu Normal University, Jinan, ChinaSchool of Life Sciences, Qilu Normal University, Jinan, ChinaSchool of Life Sciences, Qilu Normal University, Jinan, ChinaSchool of Life Sciences, Qilu Normal University, Jinan, ChinaSchool of Life Sciences, Qilu Normal University, Jinan, ChinaSchool of Life Sciences, Qilu Normal University, Jinan, ChinaKey Laboratory of Animal Resistance, School of Life Sciences, Shandong Normal University, Jinan, ChinaKey Laboratory of Animal Resistance, School of Life Sciences, Shandong Normal University, Jinan, ChinaHydrogen sulfide (H2S) is a neuromodulator in the central nervous system. However, the physiological role of H2S in the nucleus ambiguus (NA) has rarely been reported. This research aimed to elucidate the role of H2S in the regulation of gastrointestinal motility in rats. Male Wistar rats were randomly assigned to sodium hydrosulfide (NaHS; 4 and 8 nmol) groups, physiological saline (PS) group, capsazepine (10 pmol) + NaHS (4 nmol) group, L703606 (4 nmol) + NaHS (4 nmol) group, and pyrrolidine dithiocarbamate (PDTC, 4 nmol) + NaHS (4 nmol) group. Gastrointestinal motility curves before and after the injection were recorded using a latex balloon attached with a pressure transducer, which was introduced into the pylorus through gastric fundus. The results demonstrated that NaHS (4 and 8 nmol), an exogenous H2S donor, remarkably suppressed gastrointestinal motility in the NA of rats (P < 0.01). The suppressive effect of NaHS on gastrointestinal motility could be prevented by capsazepine, a transient receptor potential vanilloid 1 (TRPV1) antagonist, and PDTC, a NF-κB inhibitor. However, the same amount of PS did not induce significant changes in gastrointestinal motility (P > 0.05). Our findings indicate that NaHS within the NA can remarkably suppress gastrointestinal motility in rats, possibly through TRPV1 channels and NF-κB-dependent mechanism.https://www.frontiersin.org/article/10.3389/fphys.2020.545184/fullnucleus ambiguushydrogen sulfidegastric motilityTRPV1NF-κB |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hongzhao Sun Haikun Ding Yuan Shi Chenyu Li Haoran Jin Xiaoyue Yang Zhaosong Chen Pengpeng Tian Jianping Zhu Haiji Sun |
spellingShingle |
Hongzhao Sun Haikun Ding Yuan Shi Chenyu Li Haoran Jin Xiaoyue Yang Zhaosong Chen Pengpeng Tian Jianping Zhu Haiji Sun Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats Frontiers in Physiology nucleus ambiguus hydrogen sulfide gastric motility TRPV1 NF-κB |
author_facet |
Hongzhao Sun Haikun Ding Yuan Shi Chenyu Li Haoran Jin Xiaoyue Yang Zhaosong Chen Pengpeng Tian Jianping Zhu Haiji Sun |
author_sort |
Hongzhao Sun |
title |
Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats |
title_short |
Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats |
title_full |
Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats |
title_fullStr |
Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats |
title_full_unstemmed |
Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats |
title_sort |
exogenous hydrogen sulfide within the nucleus ambiguus inhibits gastrointestinal motility in rats |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2020-09-01 |
description |
Hydrogen sulfide (H2S) is a neuromodulator in the central nervous system. However, the physiological role of H2S in the nucleus ambiguus (NA) has rarely been reported. This research aimed to elucidate the role of H2S in the regulation of gastrointestinal motility in rats. Male Wistar rats were randomly assigned to sodium hydrosulfide (NaHS; 4 and 8 nmol) groups, physiological saline (PS) group, capsazepine (10 pmol) + NaHS (4 nmol) group, L703606 (4 nmol) + NaHS (4 nmol) group, and pyrrolidine dithiocarbamate (PDTC, 4 nmol) + NaHS (4 nmol) group. Gastrointestinal motility curves before and after the injection were recorded using a latex balloon attached with a pressure transducer, which was introduced into the pylorus through gastric fundus. The results demonstrated that NaHS (4 and 8 nmol), an exogenous H2S donor, remarkably suppressed gastrointestinal motility in the NA of rats (P < 0.01). The suppressive effect of NaHS on gastrointestinal motility could be prevented by capsazepine, a transient receptor potential vanilloid 1 (TRPV1) antagonist, and PDTC, a NF-κB inhibitor. However, the same amount of PS did not induce significant changes in gastrointestinal motility (P > 0.05). Our findings indicate that NaHS within the NA can remarkably suppress gastrointestinal motility in rats, possibly through TRPV1 channels and NF-κB-dependent mechanism. |
topic |
nucleus ambiguus hydrogen sulfide gastric motility TRPV1 NF-κB |
url |
https://www.frontiersin.org/article/10.3389/fphys.2020.545184/full |
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