Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats
Treatment for peripheral nerve injury remains limited. The inherent differences between motor and sensory nerve fibers in peripheral nerves should be considered to improve the effects of clinical treatment of peripheral nerve injury. In this study, we investigated the differences in protein expressi...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-04-01
|
Series: | Frontiers in Neuroanatomy |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fnana.2020.00016/full |
id |
doaj-0f16de81c4f54c1586a01ca9a2b7b2dd |
---|---|
record_format |
Article |
spelling |
doaj-0f16de81c4f54c1586a01ca9a2b7b2dd2020-11-25T02:30:56ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292020-04-011410.3389/fnana.2020.00016531946Differences in the Structure and Protein Expression of Femoral Nerve Branches in RatsShuai Wei0Shuai Wei1Shuai Wei2Qian Hu3Xiaoqing Cheng4Xiaoqing Cheng5Jianxiong Ma6Xuezhen Liang7Jiang Peng8Jiang Peng9Wenjing Xu10Xun Sun11Gonghai Han12Xinlong Ma13Yu Wang14Yu Wang15Tianjin Hospital Tianjin University, Tianjin, ChinaInstitute of Orthopedics, Chinese People’s Liberation Army (PLA) General Hospital, Beijing, ChinaCo-Innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaDepartment of Geriatrics, The Second People’s Hospital of Nantong, Nantong, ChinaInstitute of Orthopedics, Chinese People’s Liberation Army (PLA) General Hospital, Beijing, ChinaCo-Innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaTianjin Hospital Tianjin University, Tianjin, ChinaThe First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Shandong, ChinaInstitute of Orthopedics, Chinese People’s Liberation Army (PLA) General Hospital, Beijing, ChinaCo-Innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaInstitute of Orthopedics, Chinese People’s Liberation Army (PLA) General Hospital, Beijing, ChinaTianjin Hospital Tianjin University, Tianjin, ChinaThe First People’s Hospital of Yunnan Province, Kunming, ChinaTianjin Hospital Tianjin University, Tianjin, ChinaInstitute of Orthopedics, Chinese People’s Liberation Army (PLA) General Hospital, Beijing, ChinaCo-Innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaTreatment for peripheral nerve injury remains limited. The inherent differences between motor and sensory nerve fibers in peripheral nerves should be considered to improve the effects of clinical treatment of peripheral nerve injury. In this study, we investigated the differences in protein expression and ultrastructure between the cutaneous and muscular branches of the femoral nerve in rats. Our results suggest that the cutaneous branch of the femoral nerve mainly contains sensory nerve fibers and few motor nerve fibers; Correspondingly, many motor nerve fibers and few sensory nerve fibers were observed in the muscular branch of the femoral nerve, which indicate that two branches of femoral nerve are mixed nerve. The mean thickness of the myelin sheath and basement membrane of the medullated fibers in the muscular branch of the femoral nerve was greater than that of the cutaneous branch of the femoral nerve. However, the cutaneous branch has a larger G-ratio. Gene Ontology enrichment analysis revealed that the cellular component term extracellular space was the most highly enriched, and more genes were upregulated in the muscular branch of the femoral nerve. Meanwhile, the expression of key proteins were validated by Western Blot, and immunofluorescence targets the expression of key proteins, which is consistent with the enrichment analysis of Gene Ontology. In conclusion, inherent differences in protein expression and ultrastructure were observed between the cutaneous and muscular branches of the femoral nerve in rats, which should be considered in future studies on the treatment of peripheral nerve injuries.https://www.frontiersin.org/article/10.3389/fnana.2020.00016/fullcutaneous branchfemoral nervemuscular branchprotein expressionG-ratioultrastructure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuai Wei Shuai Wei Shuai Wei Qian Hu Xiaoqing Cheng Xiaoqing Cheng Jianxiong Ma Xuezhen Liang Jiang Peng Jiang Peng Wenjing Xu Xun Sun Gonghai Han Xinlong Ma Yu Wang Yu Wang |
spellingShingle |
Shuai Wei Shuai Wei Shuai Wei Qian Hu Xiaoqing Cheng Xiaoqing Cheng Jianxiong Ma Xuezhen Liang Jiang Peng Jiang Peng Wenjing Xu Xun Sun Gonghai Han Xinlong Ma Yu Wang Yu Wang Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats Frontiers in Neuroanatomy cutaneous branch femoral nerve muscular branch protein expression G-ratio ultrastructure |
author_facet |
Shuai Wei Shuai Wei Shuai Wei Qian Hu Xiaoqing Cheng Xiaoqing Cheng Jianxiong Ma Xuezhen Liang Jiang Peng Jiang Peng Wenjing Xu Xun Sun Gonghai Han Xinlong Ma Yu Wang Yu Wang |
author_sort |
Shuai Wei |
title |
Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats |
title_short |
Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats |
title_full |
Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats |
title_fullStr |
Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats |
title_full_unstemmed |
Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats |
title_sort |
differences in the structure and protein expression of femoral nerve branches in rats |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroanatomy |
issn |
1662-5129 |
publishDate |
2020-04-01 |
description |
Treatment for peripheral nerve injury remains limited. The inherent differences between motor and sensory nerve fibers in peripheral nerves should be considered to improve the effects of clinical treatment of peripheral nerve injury. In this study, we investigated the differences in protein expression and ultrastructure between the cutaneous and muscular branches of the femoral nerve in rats. Our results suggest that the cutaneous branch of the femoral nerve mainly contains sensory nerve fibers and few motor nerve fibers; Correspondingly, many motor nerve fibers and few sensory nerve fibers were observed in the muscular branch of the femoral nerve, which indicate that two branches of femoral nerve are mixed nerve. The mean thickness of the myelin sheath and basement membrane of the medullated fibers in the muscular branch of the femoral nerve was greater than that of the cutaneous branch of the femoral nerve. However, the cutaneous branch has a larger G-ratio. Gene Ontology enrichment analysis revealed that the cellular component term extracellular space was the most highly enriched, and more genes were upregulated in the muscular branch of the femoral nerve. Meanwhile, the expression of key proteins were validated by Western Blot, and immunofluorescence targets the expression of key proteins, which is consistent with the enrichment analysis of Gene Ontology. In conclusion, inherent differences in protein expression and ultrastructure were observed between the cutaneous and muscular branches of the femoral nerve in rats, which should be considered in future studies on the treatment of peripheral nerve injuries. |
topic |
cutaneous branch femoral nerve muscular branch protein expression G-ratio ultrastructure |
url |
https://www.frontiersin.org/article/10.3389/fnana.2020.00016/full |
work_keys_str_mv |
AT shuaiwei differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT shuaiwei differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT shuaiwei differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT qianhu differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT xiaoqingcheng differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT xiaoqingcheng differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT jianxiongma differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT xuezhenliang differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT jiangpeng differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT jiangpeng differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT wenjingxu differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT xunsun differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT gonghaihan differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT xinlongma differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT yuwang differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats AT yuwang differencesinthestructureandproteinexpressionoffemoralnervebranchesinrats |
_version_ |
1724826746796113920 |