PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism
Polyunsaturated fatty acids (PUFAs) are the main components of cell membrane affecting its fluidity, signaling processes and play a vital role in muscle cell development. The effects of docosahexaenoic acid (DHA) on myogenesis are well known, while the effects of arachidonic acid (AA) are largely un...
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doaj-0f29b792ea8a4e84bd54952567981a292021-01-29T00:05:43ZengMDPI AGGenes2073-44252021-01-011219219210.3390/genes12020192PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy MetabolismMarua Abu Risha0Puntita Siengdee1Dirk Dannenberger2Klaus Wimmers3Siriluck Ponsuksili4Functional Genome Analysis Research Unit, Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, GermanyFunctional Genome Analysis Research Unit, Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, GermanyLipid Metabolism and Muscular Adaptation Workgroup, Institute of Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, GermanyGenomics Research Unit, Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, GermanyFunctional Genome Analysis Research Unit, Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, GermanyPolyunsaturated fatty acids (PUFAs) are the main components of cell membrane affecting its fluidity, signaling processes and play a vital role in muscle cell development. The effects of docosahexaenoic acid (DHA) on myogenesis are well known, while the effects of arachidonic acid (AA) are largely unclear. The purpose of this study is to evaluate the effect of two PUFAs (DHA and AA) on cell fate during myogenic processes, Wnt signaling and energy metabolism by using the C2C12 cells. The cells were treated with different concentrations of AA or DHA for 48 h during the differentiation period. PUFA treatment increased mRNA level of myogenic factor 5 (<i>Myf5</i>), which is involved in early stage of myoblast proliferation. Additionally, PUFA treatment prevented myoblast differentiation, indicated by decreased myotube fusion index and differentiation index in parallel with reduced mRNA levels of myogenin (<i>MyoG</i>). After PUFA withdrawal, some changes in cell morphology and myosin heavy chain mRNA levels were still observed. Expression of genes associated with Wnt signaling pathway, and energy metabolism changed in PUFA treatment in a dose and time dependent manner. Our data suggests that PUFAs affect the transition of C2C12 cells from proliferation to differentiation phase by prolonging proliferation and preventing differentiation.https://www.mdpi.com/2073-4425/12/2/192C2C12energy metabolismglycolysisoxidative phosphorylationPUFAfatty acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marua Abu Risha Puntita Siengdee Dirk Dannenberger Klaus Wimmers Siriluck Ponsuksili |
spellingShingle |
Marua Abu Risha Puntita Siengdee Dirk Dannenberger Klaus Wimmers Siriluck Ponsuksili PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism Genes C2C12 energy metabolism glycolysis oxidative phosphorylation PUFA fatty acids |
author_facet |
Marua Abu Risha Puntita Siengdee Dirk Dannenberger Klaus Wimmers Siriluck Ponsuksili |
author_sort |
Marua Abu Risha |
title |
PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism |
title_short |
PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism |
title_full |
PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism |
title_fullStr |
PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism |
title_full_unstemmed |
PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism |
title_sort |
pufa treatment affects c2c12 myocyte differentiation, myogenesis related genes and energy metabolism |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2021-01-01 |
description |
Polyunsaturated fatty acids (PUFAs) are the main components of cell membrane affecting its fluidity, signaling processes and play a vital role in muscle cell development. The effects of docosahexaenoic acid (DHA) on myogenesis are well known, while the effects of arachidonic acid (AA) are largely unclear. The purpose of this study is to evaluate the effect of two PUFAs (DHA and AA) on cell fate during myogenic processes, Wnt signaling and energy metabolism by using the C2C12 cells. The cells were treated with different concentrations of AA or DHA for 48 h during the differentiation period. PUFA treatment increased mRNA level of myogenic factor 5 (<i>Myf5</i>), which is involved in early stage of myoblast proliferation. Additionally, PUFA treatment prevented myoblast differentiation, indicated by decreased myotube fusion index and differentiation index in parallel with reduced mRNA levels of myogenin (<i>MyoG</i>). After PUFA withdrawal, some changes in cell morphology and myosin heavy chain mRNA levels were still observed. Expression of genes associated with Wnt signaling pathway, and energy metabolism changed in PUFA treatment in a dose and time dependent manner. Our data suggests that PUFAs affect the transition of C2C12 cells from proliferation to differentiation phase by prolonging proliferation and preventing differentiation. |
topic |
C2C12 energy metabolism glycolysis oxidative phosphorylation PUFA fatty acids |
url |
https://www.mdpi.com/2073-4425/12/2/192 |
work_keys_str_mv |
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