Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate

• Main Authors: Patricia Braunig, Rafael Diego da Rosa, Caroline Heidrich Seibert, Mariana Borsa, Patricia Hermes Stoco, Edmundo Carlos Grisard, Aguinaldo Roberto Pinto
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2011-04-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: WSSV; VP28; recombinant protein; penaeid shrimp
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200023

In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil.