Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate
In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was...
Main Authors: | Patricia Braunig, Rafael Diego da Rosa, Caroline Heidrich Seibert, Mariana Borsa, Patricia Hermes Stoco, Edmundo Carlos Grisard, Aguinaldo Roberto Pinto |
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Format: | Article |
Language: | English |
Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2011-04-01
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Series: | Brazilian Archives of Biology and Technology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200023 |
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