Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate

In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was...

Full description

Bibliographic Details
Main Authors: Patricia Braunig, Rafael Diego da Rosa, Caroline Heidrich Seibert, Mariana Borsa, Patricia Hermes Stoco, Edmundo Carlos Grisard, Aguinaldo Roberto Pinto
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2011-04-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200023

Similar Items