Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>gene
<p>Abstract</p> <p>Background</p> <p>The stromal component of the thymic microenvironment is critical for T lymphocyte generation. Thymocyte differentiation involves a cascade of coordinated stromal genes controlling thymocyte survival, lineage commitment and selection....
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doaj-0f31000561244f918f6d29ff1460617a2020-11-25T02:27:12ZengBMCBMC Genomics1471-21642004-07-01514110.1186/1471-2164-5-41Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>geneMattei Marie-GenevièveVictorero GenevièveSaade MurielleGranjeaud SamuelPuthier DenisIrla MagaliNguyen Catherine<p>Abstract</p> <p>Background</p> <p>The stromal component of the thymic microenvironment is critical for T lymphocyte generation. Thymocyte differentiation involves a cascade of coordinated stromal genes controlling thymocyte survival, lineage commitment and selection. The "Stromal Protein Associated with Thymii And Lymph-node" (<it>Spatial</it>) gene encodes a putative transcription factor which may be involved in T-cell development. In the testis, the <it>Spatial </it>gene is also expressed by round spermatids during spermatogenesis.</p> <p>Results</p> <p>The <it>Spatial </it>gene maps to the B3-B4 region of murine chromosome 10 corresponding to the human syntenic region 10q22.1. The mouse <it>Spatial </it>genomic DNA is organised into 10 exons and is alternatively spliced to generate two short isoforms (<it>Spatial</it>-α and -γ) and two other long isoforms (<it>Spatial</it>-δ and -ε) comprising 5 additional exons on the 3' site. Here, we report the cloning of a new short isoform, <it>Spatial</it>-β, which differs from other isoforms by an additional alternative exon of 69 bases. This new exon encodes an interesting proline-rich signature that could confer to the 34 kDa Spatial-β protein a particular function. By quantitative TaqMan RT-PCR, we have shown that the short isoforms are highly expressed in the thymus while the long isoforms are highly expressed in the testis. We further examined the inter-species conservation of <it>Spatial </it>between several mammals and identified that the protein which is rich in proline and positive amino acids, is highly conserved.</p> <p>Conclusions</p> <p>The <it>Spatial </it>gene generates at least five alternative spliced variants: three short isoforms (<it>Spatial</it>-α, -β and -γ) highly expressed in the thymus and two long isoforms (<it>Spatial</it>-δ and -ε) highly expressed in the testis. These alternative spliced variants could have a tissue specific function.</p> http://www.biomedcentral.com/1471-2164/5/41 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mattei Marie-Geneviève Victorero Geneviève Saade Murielle Granjeaud Samuel Puthier Denis Irla Magali Nguyen Catherine |
spellingShingle |
Mattei Marie-Geneviève Victorero Geneviève Saade Murielle Granjeaud Samuel Puthier Denis Irla Magali Nguyen Catherine Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>gene BMC Genomics |
author_facet |
Mattei Marie-Geneviève Victorero Geneviève Saade Murielle Granjeaud Samuel Puthier Denis Irla Magali Nguyen Catherine |
author_sort |
Mattei Marie-Geneviève |
title |
Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>gene |
title_short |
Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>gene |
title_full |
Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>gene |
title_fullStr |
Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>gene |
title_full_unstemmed |
Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>Spatial </it>gene |
title_sort |
genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse <it>spatial </it>gene |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2004-07-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The stromal component of the thymic microenvironment is critical for T lymphocyte generation. Thymocyte differentiation involves a cascade of coordinated stromal genes controlling thymocyte survival, lineage commitment and selection. The "Stromal Protein Associated with Thymii And Lymph-node" (<it>Spatial</it>) gene encodes a putative transcription factor which may be involved in T-cell development. In the testis, the <it>Spatial </it>gene is also expressed by round spermatids during spermatogenesis.</p> <p>Results</p> <p>The <it>Spatial </it>gene maps to the B3-B4 region of murine chromosome 10 corresponding to the human syntenic region 10q22.1. The mouse <it>Spatial </it>genomic DNA is organised into 10 exons and is alternatively spliced to generate two short isoforms (<it>Spatial</it>-α and -γ) and two other long isoforms (<it>Spatial</it>-δ and -ε) comprising 5 additional exons on the 3' site. Here, we report the cloning of a new short isoform, <it>Spatial</it>-β, which differs from other isoforms by an additional alternative exon of 69 bases. This new exon encodes an interesting proline-rich signature that could confer to the 34 kDa Spatial-β protein a particular function. By quantitative TaqMan RT-PCR, we have shown that the short isoforms are highly expressed in the thymus while the long isoforms are highly expressed in the testis. We further examined the inter-species conservation of <it>Spatial </it>between several mammals and identified that the protein which is rich in proline and positive amino acids, is highly conserved.</p> <p>Conclusions</p> <p>The <it>Spatial </it>gene generates at least five alternative spliced variants: three short isoforms (<it>Spatial</it>-α, -β and -γ) highly expressed in the thymus and two long isoforms (<it>Spatial</it>-δ and -ε) highly expressed in the testis. These alternative spliced variants could have a tissue specific function.</p> |
url |
http://www.biomedcentral.com/1471-2164/5/41 |
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