DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia
Background and Aim: Lymphatic filariasis (LF) is a lesser-known parasitic disease, which contributes to significant decreases in overall health. This study investigated the presence of Brugia malayi in mosquitoes collected in the South Borneo Province, Indonesia. Materials and Methods: Mosquitoes...
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doaj-0f36844d17894981af967e62c51a36112021-08-02T12:03:11ZengVeterinary WorldVeterinary World0972-89882231-09162020-05-01135996100010.14202/vetworld.2020.996-1000DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, IndonesiaSupriyono Supriyono0https://orcid.org/0000-0002-5504-8824Suriyani Tan1https://orcid.org/0000-0002-5975-7089Division of Parasitology and Medical Entomology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, West Java, Indonesia.Department of Parasitology, Faculty of Medicine, Trisakti University, Jakarta, Indonesia.Background and Aim: Lymphatic filariasis (LF) is a lesser-known parasitic disease, which contributes to significant decreases in overall health. This study investigated the presence of Brugia malayi in mosquitoes collected in the South Borneo Province, Indonesia. Materials and Methods: Mosquitoes were collected through bare leg collection methods after sunset in several areas of the Hukai and Gulinggang villages in the Balangan District. The collected mosquitoes were identified based on morphological features and dissected to find microfilaria and then pooled through species for polymerase chain reaction (PCR) microfilaria detection. Results: A total of 837 female mosquitoes consisting of at least 14 species were selected; they were dissected, and no microfilariae were found. Mosquitoes were divided into 69 pools for PCR analysis. PCR revealed that 8.7% (6/69) of the pools were positive for B. malayi, including Mansonia annulifera (4 pools), Aedes albopictus (1 pool), and Culex tritaeniorhynchus (1 pool). Conclusion: These results suggested that mosquito dissection was not an optimum method for finding microfilaria. M. annulifera, C. tritaeniorhynchus, and A. albopictus mosquitoes might play an important role in the transmission of LF in the Balangan District. Information from this study could be used for the prevention of transmission or vector control programs in Indonesia.http://www.veterinaryworld.org/Vol.13/May-2020/24.pdfbrugia malayilymphatic filariasispolymerase chain reaction |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Supriyono Supriyono Suriyani Tan |
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Supriyono Supriyono Suriyani Tan DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia Veterinary World brugia malayi lymphatic filariasis polymerase chain reaction |
author_facet |
Supriyono Supriyono Suriyani Tan |
author_sort |
Supriyono Supriyono |
title |
DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia |
title_short |
DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia |
title_full |
DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia |
title_fullStr |
DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia |
title_full_unstemmed |
DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia |
title_sort |
dna of brugia malayi detected in several mosquito species collected from balangan district, south borneo province, indonesia |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2020-05-01 |
description |
Background and Aim: Lymphatic filariasis (LF) is a lesser-known parasitic disease, which contributes to significant decreases in overall health. This study investigated the presence of Brugia malayi in mosquitoes collected in the South Borneo Province, Indonesia.
Materials and Methods: Mosquitoes were collected through bare leg collection methods after sunset in several areas of the Hukai and Gulinggang villages in the Balangan District. The collected mosquitoes were identified based on morphological features and dissected to find microfilaria and then pooled through species for polymerase chain reaction (PCR) microfilaria detection.
Results: A total of 837 female mosquitoes consisting of at least 14 species were selected; they were dissected, and no microfilariae were found. Mosquitoes were divided into 69 pools for PCR analysis. PCR revealed that 8.7% (6/69) of the pools were positive for B. malayi, including Mansonia annulifera (4 pools), Aedes albopictus (1 pool), and Culex tritaeniorhynchus (1 pool).
Conclusion: These results suggested that mosquito dissection was not an optimum method for finding microfilaria. M. annulifera, C. tritaeniorhynchus, and A. albopictus mosquitoes might play an important role in the transmission of LF in the Balangan District. Information from this study could be used for the prevention of transmission or vector control programs in Indonesia. |
topic |
brugia malayi lymphatic filariasis polymerase chain reaction |
url |
http://www.veterinaryworld.org/Vol.13/May-2020/24.pdf |
work_keys_str_mv |
AT supriyonosupriyono dnaofbrugiamalayidetectedinseveralmosquitospeciescollectedfrombalangandistrictsouthborneoprovinceindonesia AT suriyanitan dnaofbrugiamalayidetectedinseveralmosquitospeciescollectedfrombalangandistrictsouthborneoprovinceindonesia |
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