Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy
Excited-state singlet oxygen (1O2), generated during photodynamic therapy (PDT), is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers. Its relative concentration in cells or tissues can be measured directly through its near-infrared (NIR) luminescence e...
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doaj-0f46a408487e42ec8d1a958dc2f5dfce2020-11-24T23:23:20ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052015-01-01811540006-11540006-710.1142/S179354581540006410.1142/S1793545815400064Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapyBrian C. Wilson0Michael S. Patterson1Buhong Li2Mark T. Jarvi3Department of Medical Biophysics, University of Toronto/University Health Network, Toronto, Ontario M5G 1L7, CanadaJuravinski Cancer Centre and McMaster University, Hamilton, Ontario L8V 5C2, CanadaMOE Key Laboratory of OptoElectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian 350007, P. R. ChinaDepartment of Medical Biophysics, University of Toronto/University Health Network, Toronto, Ontario M5G 1L7, CanadaExcited-state singlet oxygen (1O2), generated during photodynamic therapy (PDT), is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers. Its relative concentration in cells or tissues can be measured directly through its near-infrared (NIR) luminescence emission, which has correlated well with in vitro cell and in vivo normal skin treatment responses. Here, its correlation with the response of tumor tissue in vivo is examined, using the photosensitizer meso-tetrahydroxyphenylchlorin (mTHPC) in an animal model comprising luciferase- and green fluorescent protein (GFP)-transduced gliosarcoma grown in a dorsal window chamber. The change in the bioluminescence signal, imaged pretreatment and at 2, 5 and 9 d post treatment, was used as a quantitative measure of the tumor response, which was classified in individual tumors as "non", "moderate" and "strong" in order to reduce the variance in the data. Plotting the bioluminescence-based response vs the 1O2 counts demonstrated clear correlation, indicating that 1O2 luminescence provides a valid dosimetric technique for PDT in tumor tissue.http://www.worldscientific.com/doi/pdf/10.1142/S1793545815400064Photodynamic therapysinglet oxygen luminescence dosimetrybioluminescence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Brian C. Wilson Michael S. Patterson Buhong Li Mark T. Jarvi |
spellingShingle |
Brian C. Wilson Michael S. Patterson Buhong Li Mark T. Jarvi Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy Journal of Innovative Optical Health Sciences Photodynamic therapy singlet oxygen luminescence dosimetry bioluminescence |
author_facet |
Brian C. Wilson Michael S. Patterson Buhong Li Mark T. Jarvi |
author_sort |
Brian C. Wilson |
title |
Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy |
title_short |
Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy |
title_full |
Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy |
title_fullStr |
Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy |
title_full_unstemmed |
Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy |
title_sort |
correlation of in vivo tumor response and singlet oxygen luminescence detection in mthpc-mediated photodynamic therapy |
publisher |
World Scientific Publishing |
series |
Journal of Innovative Optical Health Sciences |
issn |
1793-5458 1793-7205 |
publishDate |
2015-01-01 |
description |
Excited-state singlet oxygen (1O2), generated during photodynamic therapy (PDT), is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers. Its relative concentration in cells or tissues can be measured directly through its near-infrared (NIR) luminescence emission, which has correlated well with in vitro cell and in vivo normal skin treatment responses. Here, its correlation with the response of tumor tissue in vivo is examined, using the photosensitizer meso-tetrahydroxyphenylchlorin (mTHPC) in an animal model comprising luciferase- and green fluorescent protein (GFP)-transduced gliosarcoma grown in a dorsal window chamber. The change in the bioluminescence signal, imaged pretreatment and at 2, 5 and 9 d post treatment, was used as a quantitative measure of the tumor response, which was classified in individual tumors as "non", "moderate" and "strong" in order to reduce the variance in the data. Plotting the bioluminescence-based response vs the 1O2 counts demonstrated clear correlation, indicating that 1O2 luminescence provides a valid dosimetric technique for PDT in tumor tissue. |
topic |
Photodynamic therapy singlet oxygen luminescence dosimetry bioluminescence |
url |
http://www.worldscientific.com/doi/pdf/10.1142/S1793545815400064 |
work_keys_str_mv |
AT briancwilson correlationofinvivotumorresponseandsingletoxygenluminescencedetectioninmthpcmediatedphotodynamictherapy AT michaelspatterson correlationofinvivotumorresponseandsingletoxygenluminescencedetectioninmthpcmediatedphotodynamictherapy AT buhongli correlationofinvivotumorresponseandsingletoxygenluminescencedetectioninmthpcmediatedphotodynamictherapy AT marktjarvi correlationofinvivotumorresponseandsingletoxygenluminescencedetectioninmthpcmediatedphotodynamictherapy |
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