Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis
This study investigates endocytosis of Saccharomyces cerevisiae α-factor receptor and the role that receptor oligomerization plays in this process. α-factor receptor contains signal sequences in the cytoplasmic C-terminal domain that are essential for ligand-mediated endocytosis. In an endocytosis c...
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2014-03-01
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doaj-0f64f9dc0aec4bf6ad804a66a7f15b512021-06-02T09:27:01ZengThe Company of BiologistsBiology Open2046-63902014-03-013429730610.1242/bio.2014686620146866Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosisChien-I Chang0Kimberly A. Schandel1Duane D. Jenness Present address: Department of Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01655, USA. Present address: Department of Natural Sciences, Assumption College, 500 Salisbury Street, Worcester, MA 01609, USA. This study investigates endocytosis of Saccharomyces cerevisiae α-factor receptor and the role that receptor oligomerization plays in this process. α-factor receptor contains signal sequences in the cytoplasmic C-terminal domain that are essential for ligand-mediated endocytosis. In an endocytosis complementation assay, we found that oligomeric complexes of the receptor undergo ligand-mediated endocytosis when the α-factor binding site and the endocytosis signal sequences are located in different receptors. Both in vitro and in vivo assays suggested that ligand-induced conformational changes in one Ste2 subunit do not affect neighboring subunits. Therefore, recognition of the endocytosis signal sequence and recognition of the ligand-induced conformational change are likely to be two independent events.http://bio.biologists.org/content/3/4/297G-protein coupled receptorsReceptor oligomersLigand-mediated endocytosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chien-I Chang Kimberly A. Schandel Duane D. Jenness |
spellingShingle |
Chien-I Chang Kimberly A. Schandel Duane D. Jenness Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis Biology Open G-protein coupled receptors Receptor oligomers Ligand-mediated endocytosis |
author_facet |
Chien-I Chang Kimberly A. Schandel Duane D. Jenness |
author_sort |
Chien-I Chang |
title |
Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis |
title_short |
Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis |
title_full |
Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis |
title_fullStr |
Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis |
title_full_unstemmed |
Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis |
title_sort |
interaction among saccharomyces cerevisiae pheromone receptors during endocytosis |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2014-03-01 |
description |
This study investigates endocytosis of Saccharomyces cerevisiae α-factor receptor and the role that receptor oligomerization plays in this process. α-factor receptor contains signal sequences in the cytoplasmic C-terminal domain that are essential for ligand-mediated endocytosis. In an endocytosis complementation assay, we found that oligomeric complexes of the receptor undergo ligand-mediated endocytosis when the α-factor binding site and the endocytosis signal sequences are located in different receptors. Both in vitro and in vivo assays suggested that ligand-induced conformational changes in one Ste2 subunit do not affect neighboring subunits. Therefore, recognition of the endocytosis signal sequence and recognition of the ligand-induced conformational change are likely to be two independent events. |
topic |
G-protein coupled receptors Receptor oligomers Ligand-mediated endocytosis |
url |
http://bio.biologists.org/content/3/4/297 |
work_keys_str_mv |
AT chienichang interactionamongsaccharomycescerevisiaepheromonereceptorsduringendocytosis AT kimberlyaschandel interactionamongsaccharomycescerevisiaepheromonereceptorsduringendocytosis AT duanedjenness interactionamongsaccharomycescerevisiaepheromonereceptorsduringendocytosis |
_version_ |
1721405748782563328 |