Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles
The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovaria...
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doaj-0f679e4cef5f433daf3af579231f34c42020-11-25T03:57:23ZengMDPI AGMaterials1996-19442020-08-01133759375910.3390/ma13173759Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and FolliclesGiulia Pascoletti0Maddalena Di Nardo1Gionata Fragomeni2Vincenza Barbato3Teresa Capriglione4Roberto Gualtieri5Riccardo Talevi6Gerardo Catapano7Elisabetta M. Zanetti8Department of Engineering, University of Perugia, 06125 Perugia, ItalyDepartment of Biology, University of Naples Federico II, 80100 Naples, ItalyDepartment of Medical and Surgical Sciences, Magna Graecia University, 88100 Catanzaro, ItalyDepartment of Biology, University of Naples Federico II, 80100 Naples, ItalyDepartment of Biology, University of Naples Federico II, 80100 Naples, ItalyDepartment of Biology, University of Naples Federico II, 80100 Naples, ItalyDepartment of Biology, University of Naples Federico II, 80100 Naples, ItalyDepartment of Mechanical, Energy and Management Engineering, University of Calabria, 87030 Rende (CS), ItalyDepartment of Engineering, University of Perugia, 06125 Perugia, ItalyThe ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage.https://www.mdpi.com/1996-1944/13/17/3759ovarian tissuecreepelastic modulustensile testviscous behaviorbiomechanics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giulia Pascoletti Maddalena Di Nardo Gionata Fragomeni Vincenza Barbato Teresa Capriglione Roberto Gualtieri Riccardo Talevi Gerardo Catapano Elisabetta M. Zanetti |
spellingShingle |
Giulia Pascoletti Maddalena Di Nardo Gionata Fragomeni Vincenza Barbato Teresa Capriglione Roberto Gualtieri Riccardo Talevi Gerardo Catapano Elisabetta M. Zanetti Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles Materials ovarian tissue creep elastic modulus tensile test viscous behavior biomechanics |
author_facet |
Giulia Pascoletti Maddalena Di Nardo Gionata Fragomeni Vincenza Barbato Teresa Capriglione Roberto Gualtieri Riccardo Talevi Gerardo Catapano Elisabetta M. Zanetti |
author_sort |
Giulia Pascoletti |
title |
Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles |
title_short |
Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles |
title_full |
Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles |
title_fullStr |
Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles |
title_full_unstemmed |
Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles |
title_sort |
dynamic characterization of the biomechanical behaviour of bovine ovarian cortical tissue and its short-term effect on ovarian tissue and follicles |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2020-08-01 |
description |
The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage. |
topic |
ovarian tissue creep elastic modulus tensile test viscous behavior biomechanics |
url |
https://www.mdpi.com/1996-1944/13/17/3759 |
work_keys_str_mv |
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