First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon)
Black cutworm (BCW) Agrotis ipsilon, an economically important lepidopteran insect, has attracted a great attention. Bacillus thuringiensis (Bt) is spore forming soil bacteria and is an excellent environment-friendly approach for the control of phytophagous and disease-transmitting insects. In fact,...
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doaj-0f6b8c48195547619ae170e2299d16022020-11-24T22:35:52ZengElsevierSaudi Journal of Biological Sciences1319-562X2018-03-01253441445First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon)Gamal H. Osman0Waleed J. Altaf1Ibrahim A.S. Saleh2Raya Soltane3Hussein H. Abulreesh4Ibrahim A. Arif5Ahmed M. Ramadan6Yehia A. Osman7Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia; Microbial Genetics Department, Agricultural Genetic Engineering Research Institute (AGERI), Giza, Egypt; Corresponding author at: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia.Department of Physics, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi ArabiaPrince Sultan Research Chair for Environment and Wildlife, Department of Botany & Microbiology, College of Sciences, King Saud University (KSU), Riyadh, Saudi ArabiaDepartment of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi ArabiaPrince Sultan Research Chair for Environment and Wildlife, Department of Botany & Microbiology, College of Sciences, King Saud University (KSU), Riyadh, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah 80203, Saudi Arabia; Plant Molecular Biology Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, EgyptMicrobiology Department, Faculty of Science, Mansoura University, Mansoura, EgyptBlack cutworm (BCW) Agrotis ipsilon, an economically important lepidopteran insect, has attracted a great attention. Bacillus thuringiensis (Bt) is spore forming soil bacteria and is an excellent environment-friendly approach for the control of phytophagous and disease-transmitting insects. In fact, bio-pesticide formulations and insect resistant transgenic plants based on the bacterium Bt delta-endotoxin have attracted worldwide attention as a safer alternative to harmful chemical pesticides. The major objective of the current study was to understand the mechanism of interaction of Bt toxin with its receptor molecule(s). The investigation involved the isolation, identification, and characterization of a putative receptor – vip3Aa. In addition, the kinetics of vip toxin binding to its receptor molecule was also studied. The present data suggest that Vip3Aa toxin bound specifically with high affinity to a 48-kDa protein present at the brush border membrane vesicles (BBMV) prepared from the midgut epithelial cells of BCW larvae. Keywords: Receptor, vip3Aa, Bacillus thuringiensis, BBMVhttp://www.sciencedirect.com/science/article/pii/S1319562X18300159 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gamal H. Osman Waleed J. Altaf Ibrahim A.S. Saleh Raya Soltane Hussein H. Abulreesh Ibrahim A. Arif Ahmed M. Ramadan Yehia A. Osman |
spellingShingle |
Gamal H. Osman Waleed J. Altaf Ibrahim A.S. Saleh Raya Soltane Hussein H. Abulreesh Ibrahim A. Arif Ahmed M. Ramadan Yehia A. Osman First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon) Saudi Journal of Biological Sciences |
author_facet |
Gamal H. Osman Waleed J. Altaf Ibrahim A.S. Saleh Raya Soltane Hussein H. Abulreesh Ibrahim A. Arif Ahmed M. Ramadan Yehia A. Osman |
author_sort |
Gamal H. Osman |
title |
First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon) |
title_short |
First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon) |
title_full |
First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon) |
title_fullStr |
First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon) |
title_full_unstemmed |
First report of detection of the putative receptor of Bacillus thuringiensis toxin Vip3Aa from black cutworm (Agrotis ipsilon) |
title_sort |
first report of detection of the putative receptor of bacillus thuringiensis toxin vip3aa from black cutworm (agrotis ipsilon) |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2018-03-01 |
description |
Black cutworm (BCW) Agrotis ipsilon, an economically important lepidopteran insect, has attracted a great attention. Bacillus thuringiensis (Bt) is spore forming soil bacteria and is an excellent environment-friendly approach for the control of phytophagous and disease-transmitting insects. In fact, bio-pesticide formulations and insect resistant transgenic plants based on the bacterium Bt delta-endotoxin have attracted worldwide attention as a safer alternative to harmful chemical pesticides. The major objective of the current study was to understand the mechanism of interaction of Bt toxin with its receptor molecule(s). The investigation involved the isolation, identification, and characterization of a putative receptor – vip3Aa. In addition, the kinetics of vip toxin binding to its receptor molecule was also studied. The present data suggest that Vip3Aa toxin bound specifically with high affinity to a 48-kDa protein present at the brush border membrane vesicles (BBMV) prepared from the midgut epithelial cells of BCW larvae. Keywords: Receptor, vip3Aa, Bacillus thuringiensis, BBMV |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X18300159 |
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