PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS
The aim of the research was to study the activity of poly (ADP-ribose) polymerase in small intestinal homogenate of rats with chronic carrageenan-induced gastroenterocolitis, as well as mechanisms of regulation of the enzyme in this pathology. Twenty Wistar Albino Glaxo rats were divided into two gr...
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V. N. Karazin Kharkiv National University
2017-12-01
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doaj-0f7069bde7ab493bb7857d7e480909162020-11-24T23:45:56ZengV. N. Karazin Kharkiv National UniversityJournal of V. N. Karazin Kharkiv National University: Series Medicine2313-66932313-23962017-12-01344146PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITISTkachenko A. S.Nakonechna O. A.Zhukov V. I.Gorbach T. V.Tkachenko M. O.The aim of the research was to study the activity of poly (ADP-ribose) polymerase in small intestinal homogenate of rats with chronic carrageenan-induced gastroenterocolitis, as well as mechanisms of regulation of the enzyme in this pathology. Twenty Wistar Albino Glaxo rats were divided into two groups. Animals of group 1 (n = 10) consumed 1 % carrageenan solution for 28 days, which resulted in the development of gastroenterocolitis confirmed morphologically. The control group consisted of intact animals (n = 10). The activity of poly (ADP-ribose) polymerase (PARP) in the homogenate of small intestine, as well as caspase-3, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) serum levels were determined. Obtained data were statistically processed using the Mann-Whitney U test and calculating median and interquartile range (Me, 25th–75th percentile) with the help of the GraphPad Prism 5 application. The development of carrageenan-induced gastroenterocolitis was accompanied by an increase in caspase-3, MMP-2, MMP-9 concentrations in blood serum and a decrease in the activity of PARP in small intestinal homogenates. The reduced activity of PARP in chronic carrageenan-induced gastroenterocolitis may be due to the proteolysis of this enzyme under the action of caspase-3, MMP-2, and MMP-9. http://periodicals.karazin.ua/medicine/article/view/10038/9556gastroenterocolitiscarrageenanratspoly (ADP-ribose) polymerasecaspase-3matrix metalloproteinases |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tkachenko A. S. Nakonechna O. A. Zhukov V. I. Gorbach T. V. Tkachenko M. O. |
spellingShingle |
Tkachenko A. S. Nakonechna O. A. Zhukov V. I. Gorbach T. V. Tkachenko M. O. PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS Journal of V. N. Karazin Kharkiv National University: Series Medicine gastroenterocolitis carrageenan rats poly (ADP-ribose) polymerase caspase-3 matrix metalloproteinases |
author_facet |
Tkachenko A. S. Nakonechna O. A. Zhukov V. I. Gorbach T. V. Tkachenko M. O. |
author_sort |
Tkachenko A. S. |
title |
PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS |
title_short |
PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS |
title_full |
PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS |
title_fullStr |
PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS |
title_full_unstemmed |
PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS |
title_sort |
proteolytic degradation of poly (adp-ribose) polymerase in rats with carrageenan-induced gastroenterocolitis |
publisher |
V. N. Karazin Kharkiv National University |
series |
Journal of V. N. Karazin Kharkiv National University: Series Medicine |
issn |
2313-6693 2313-2396 |
publishDate |
2017-12-01 |
description |
The aim of the research was to study the activity of poly (ADP-ribose) polymerase in small intestinal homogenate of rats with chronic carrageenan-induced gastroenterocolitis, as well as mechanisms of regulation of the enzyme in this pathology. Twenty Wistar Albino Glaxo rats were divided into two groups. Animals of group 1 (n = 10) consumed 1 % carrageenan solution for 28 days, which resulted in the development of gastroenterocolitis confirmed morphologically. The control group consisted of intact animals (n = 10). The activity of poly (ADP-ribose) polymerase (PARP) in the homogenate of small intestine, as well as caspase-3, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) serum levels were determined. Obtained data were statistically processed using the Mann-Whitney U test and calculating median and interquartile range (Me, 25th–75th percentile) with the help of the GraphPad Prism 5 application. The development of carrageenan-induced gastroenterocolitis was accompanied by an increase in caspase-3,
MMP-2, MMP-9 concentrations in blood serum and a decrease in the activity of PARP in small intestinal homogenates. The reduced activity of PARP in chronic carrageenan-induced gastroenterocolitis may be due to the proteolysis of this enzyme under the action of caspase-3, MMP-2, and MMP-9.
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topic |
gastroenterocolitis carrageenan rats poly (ADP-ribose) polymerase caspase-3 matrix metalloproteinases |
url |
http://periodicals.karazin.ua/medicine/article/view/10038/9556 |
work_keys_str_mv |
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