Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss program
This study aims to assess methylation modifications in blood cell genes induced by eicosapentaenoic acid (EPA) and/or α-lipoic acid (LA) supplementation, and their potential relationship with metabolic risk biomarkers. Healthy overweight/obese women were assigned to 4 experimental groups (control or...
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doaj-0f74ca3037004788bbed8cdf98d23c142021-04-30T07:11:02ZengElsevierJournal of Functional Foods1756-46462017-09-0136178185Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss programAna E. Huerta0José I. Riezu-Boj1Fermín I. Milagro2Elisabet Guruceaga3María J. Moreno-Aliaga4J. Alfredo Martínez5University of Navarra, Department of Nutrition, Food Science and Physiology, Pamplona, Spain; University of Navarra, Centre for Nutrition Research, Pamplona, SpainUniversity of Navarra, Department of Nutrition, Food Science and Physiology, Pamplona, Spain; University of Navarra, Centre for Nutrition Research, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, SpainUniversity of Navarra, Department of Nutrition, Food Science and Physiology, Pamplona, Spain; University of Navarra, Centre for Nutrition Research, Pamplona, Spain; Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERobn), Institute of Health Carlos III (ISCIII), Madrid, SpainNavarra Institute for Health Research (IdiSNA), Pamplona, Spain; Proteomics & Bioinformatics Unit, Centre for Applied Medical Research (CIMA), Pamplona, SpainUniversity of Navarra, Department of Nutrition, Food Science and Physiology, Pamplona, Spain; University of Navarra, Centre for Nutrition Research, Pamplona, Spain; Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERobn), Institute of Health Carlos III (ISCIII), Madrid, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain; Corresponding author at: Department of Nutrition, Food Science and Physiology, University of Navarra, C/Irunlarrea 1, 31008 Pamplona, Spain.University of Navarra, Department of Nutrition, Food Science and Physiology, Pamplona, Spain; University of Navarra, Centre for Nutrition Research, Pamplona, Spain; Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERobn), Institute of Health Carlos III (ISCIII), Madrid, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, SpainThis study aims to assess methylation modifications in blood cell genes induced by eicosapentaenoic acid (EPA) and/or α-lipoic acid (LA) supplementation, and their potential relationship with metabolic risk biomarkers. Healthy overweight/obese women were assigned to 4 experimental groups (control or groups supplemented with 1.3 g EPA/day, 0.3 g LA/day, or both), all followed a 10-week hypocaloric diet. White blood cells DNA was hybridized in Human-450K-methylation microarray. Differentially methylated CpGs (post–pre) were identified in supplemented groups, including CpG regions from NCK2, FITM2, TRRAP, RPTOR and CREBBP genes. In peripheral blood mononuclear cells (PBMC), LA upregulated NCK2, TRRAP and RPTOR mRNA, which negatively associated with changes in body weight and fat mass. Changes in cg10320884 (TRRAP) methylation site negatively correlated with changes in TRRAP mRNA in PBMC, and positively with Framingham score. Further studies are needed to better characterize the potential involvement of epigenetics in the actions of LA and EPA.http://www.sciencedirect.com/science/article/pii/S1756464617303857EpigeneticsLipoic acidMethylationOmega-3Weight loss |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana E. Huerta José I. Riezu-Boj Fermín I. Milagro Elisabet Guruceaga María J. Moreno-Aliaga J. Alfredo Martínez |
spellingShingle |
Ana E. Huerta José I. Riezu-Boj Fermín I. Milagro Elisabet Guruceaga María J. Moreno-Aliaga J. Alfredo Martínez Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss program Journal of Functional Foods Epigenetics Lipoic acid Methylation Omega-3 Weight loss |
author_facet |
Ana E. Huerta José I. Riezu-Boj Fermín I. Milagro Elisabet Guruceaga María J. Moreno-Aliaga J. Alfredo Martínez |
author_sort |
Ana E. Huerta |
title |
Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss program |
title_short |
Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss program |
title_full |
Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss program |
title_fullStr |
Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss program |
title_full_unstemmed |
Differential peripheral blood methylation by α-lipoic acid and EPA supplementation in overweight or obese women during a weight loss program |
title_sort |
differential peripheral blood methylation by α-lipoic acid and epa supplementation in overweight or obese women during a weight loss program |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2017-09-01 |
description |
This study aims to assess methylation modifications in blood cell genes induced by eicosapentaenoic acid (EPA) and/or α-lipoic acid (LA) supplementation, and their potential relationship with metabolic risk biomarkers. Healthy overweight/obese women were assigned to 4 experimental groups (control or groups supplemented with 1.3 g EPA/day, 0.3 g LA/day, or both), all followed a 10-week hypocaloric diet. White blood cells DNA was hybridized in Human-450K-methylation microarray. Differentially methylated CpGs (post–pre) were identified in supplemented groups, including CpG regions from NCK2, FITM2, TRRAP, RPTOR and CREBBP genes. In peripheral blood mononuclear cells (PBMC), LA upregulated NCK2, TRRAP and RPTOR mRNA, which negatively associated with changes in body weight and fat mass. Changes in cg10320884 (TRRAP) methylation site negatively correlated with changes in TRRAP mRNA in PBMC, and positively with Framingham score. Further studies are needed to better characterize the potential involvement of epigenetics in the actions of LA and EPA. |
topic |
Epigenetics Lipoic acid Methylation Omega-3 Weight loss |
url |
http://www.sciencedirect.com/science/article/pii/S1756464617303857 |
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