Identification of exotic North American crayfish in Europe by DNA barcoding
Several alien crayfish of North American origin have become established in Europe in recent decades, but their identification is often confusing. Our aim was to verify the taxonomic status of their European populations by DNA barcoding. We sequenced the cytochr...
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doaj-0f7bccb2972e49bc9dd338571694b9b62020-11-25T00:56:42ZengEDP SciencesKnowledge and Management of Aquatic Ecosystems1961-95022011-05-0104011110.1051/kmae/2011025kmae110022Identification of exotic North American crayfish in Europe by DNA barcodingFilipová L.Grandjean F.0Chucholl C.1Soes D. M.Petrusek A.2 Laboratoire Écologie, Évolution, Symbiose, UMR CNRS 6556, Université de Poitiers Institute of Experimental Ecology (Bio III), University of Ulm Department of Ecology, Faculty of Science, Charles University in Prague Several alien crayfish of North American origin have become established in Europe in recent decades, but their identification is often confusing. Our aim was to verify the taxonomic status of their European populations by DNA barcoding. We sequenced the cytochrome c oxidase subunit I (COI) gene fragment of individuals representing all American crayfish known from European waters, and compared the results with reference sequences from North America. Our results confirm the morphological identification of Orconectes juvenilis from a population in eastern France, and of the marbled crayfish (Marmorkrebs), i.e., a parthenogenetic form of Procambarus fallax, from south-western Germany. Sequences of most individuals of presumed Procambarus acutus from the Netherlands were similar to American P. cf. acutus, but one was divergent, closer to a sequence of a reference individual of P. cf. zonangulus. However, divergences among three American P. cf. zonangulus samples were also high, comparable to interspecific variation within cambarid species complexes. The divergence between O. immunis from Europe and America also reached values corresponding to those observed among distinct Orconectes species. Genetic variation in the American range of these crayfish should therefore be further studied. Our study shows that DNA barcoding is useful for the rapid and accurate identification of exotic crayfish in Europe, and also provides insights into overall variation within these taxa. http://dx.doi.org/10.1051/kmae/2011025COIbarcodinginvasive crayfishEuropeNorth AmericaOrconectesProcambarus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Filipová L. Grandjean F. Chucholl C. Soes D. M. Petrusek A. |
spellingShingle |
Filipová L. Grandjean F. Chucholl C. Soes D. M. Petrusek A. Identification of exotic North American crayfish in Europe by DNA barcoding Knowledge and Management of Aquatic Ecosystems COI barcoding invasive crayfish Europe North America Orconectes Procambarus |
author_facet |
Filipová L. Grandjean F. Chucholl C. Soes D. M. Petrusek A. |
author_sort |
Filipová L. |
title |
Identification of exotic North American crayfish in Europe by
DNA barcoding |
title_short |
Identification of exotic North American crayfish in Europe by
DNA barcoding |
title_full |
Identification of exotic North American crayfish in Europe by
DNA barcoding |
title_fullStr |
Identification of exotic North American crayfish in Europe by
DNA barcoding |
title_full_unstemmed |
Identification of exotic North American crayfish in Europe by
DNA barcoding |
title_sort |
identification of exotic north american crayfish in europe by
dna barcoding |
publisher |
EDP Sciences |
series |
Knowledge and Management of Aquatic Ecosystems |
issn |
1961-9502 |
publishDate |
2011-05-01 |
description |
Several alien crayfish of North American origin have become established in Europe in
recent decades, but their identification is often confusing. Our aim was to verify the
taxonomic status of their European populations by DNA barcoding. We sequenced the
cytochrome c oxidase subunit I (COI) gene fragment of individuals
representing all American crayfish known from European waters, and compared the results
with reference sequences from North America. Our results confirm the morphological
identification of Orconectes juvenilis from a population
in eastern France, and of the marbled crayfish (Marmorkrebs), i.e., a
parthenogenetic form of Procambarus fallax, from south-western Germany.
Sequences of most individuals of presumed Procambarus acutus from the
Netherlands were similar to American P. cf.
acutus, but one was divergent, closer to a sequence of a reference
individual of P. cf. zonangulus.
However, divergences among three American P. cf.
zonangulus samples were also high, comparable to interspecific
variation within cambarid species complexes. The divergence between O. immunis
from Europe and America also reached values corresponding to those observed among
distinct Orconectes species. Genetic variation in the American
range of these crayfish should therefore be further studied. Our study shows that DNA
barcoding is useful for the rapid and accurate identification of exotic crayfish in
Europe, and also provides insights into overall variation within these taxa.
|
topic |
COI barcoding invasive crayfish Europe North America Orconectes Procambarus |
url |
http://dx.doi.org/10.1051/kmae/2011025 |
work_keys_str_mv |
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