Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electro...

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Bibliographic Details
Main Authors: Tiziano Ingegnere, Francesca Romana Mariotti, Andrea Pelosi, Concetta Quintarelli, Biagio De Angelis, Nicola Tumino, Francesca Besi, Claudia Cantoni, Franco Locatelli, Paola Vacca, Lorenzo Moretta
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Immunology
Subjects:
Chimeric Antigen Receptors
chemokine receptors
NK cells
adoptive immunotherapy
electroporation
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2019.00957/full
Description
Summary:CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
ISSN:1664-3224

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