Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism
Caffeine has been demonstrated to possess anti-fibrotic activity against liver fibrosis. However, its role in renal fibrosis remained unclear. This study investigated the effects of caffeine on renal fibroblast activation induced by hypoxia (one of the inducers for renal fibrosis). BHK-21 fibroblast...
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Online Access: | http://dx.doi.org/10.1080/19336918.2019.1638691 |
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doaj-0f862681cb5446de905384890060010a2020-11-25T00:51:54ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262019-01-0113125927110.1080/19336918.2019.16386911638691Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanismAngkhana Nilnumkhum0Rattiyaporn Kanlaya1Sunisa Yoodee2Visith Thongboonkerd3Mahidol UniversityMahidol UniversityMahidol UniversityMahidol UniversityCaffeine has been demonstrated to possess anti-fibrotic activity against liver fibrosis. However, its role in renal fibrosis remained unclear. This study investigated the effects of caffeine on renal fibroblast activation induced by hypoxia (one of the inducers for renal fibrosis). BHK-21 fibroblasts were cultured under normoxia or hypoxia with or without caffeine treatment. Hypoxia increased levels of fibronectin, α-smooth muscle actin, actin stress fibers, intracellular reactive oxygen species (ROS), and oxidized proteins. However, caffeine successfully preserved all these activated fibroblast markers to their basal levels. Cellular catalase activity was dropped under hypoxic condition but could be reactivated by caffeine. Hif1a gene and stress-responsive Nrf2 signaling molecule were elevated/activated by hypoxia, but only Nrf2 could be partially recovered by caffeine. These data suggest that caffeine exhibits anti-fibrotic effect against hypoxia-induced renal fibroblast activation through its antioxidant property to eliminate intracellular ROS, at least in part, via downstream catalase and Nrf2 mechanisms.http://dx.doi.org/10.1080/19336918.2019.1638691catalasecoffeefibrogenesishypoxianrf2renal fibrosisros |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Angkhana Nilnumkhum Rattiyaporn Kanlaya Sunisa Yoodee Visith Thongboonkerd |
spellingShingle |
Angkhana Nilnumkhum Rattiyaporn Kanlaya Sunisa Yoodee Visith Thongboonkerd Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism Cell Adhesion & Migration catalase coffee fibrogenesis hypoxia nrf2 renal fibrosis ros |
author_facet |
Angkhana Nilnumkhum Rattiyaporn Kanlaya Sunisa Yoodee Visith Thongboonkerd |
author_sort |
Angkhana Nilnumkhum |
title |
Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism |
title_short |
Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism |
title_full |
Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism |
title_fullStr |
Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism |
title_full_unstemmed |
Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism |
title_sort |
caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism |
publisher |
Taylor & Francis Group |
series |
Cell Adhesion & Migration |
issn |
1933-6918 1933-6926 |
publishDate |
2019-01-01 |
description |
Caffeine has been demonstrated to possess anti-fibrotic activity against liver fibrosis. However, its role in renal fibrosis remained unclear. This study investigated the effects of caffeine on renal fibroblast activation induced by hypoxia (one of the inducers for renal fibrosis). BHK-21 fibroblasts were cultured under normoxia or hypoxia with or without caffeine treatment. Hypoxia increased levels of fibronectin, α-smooth muscle actin, actin stress fibers, intracellular reactive oxygen species (ROS), and oxidized proteins. However, caffeine successfully preserved all these activated fibroblast markers to their basal levels. Cellular catalase activity was dropped under hypoxic condition but could be reactivated by caffeine. Hif1a gene and stress-responsive Nrf2 signaling molecule were elevated/activated by hypoxia, but only Nrf2 could be partially recovered by caffeine. These data suggest that caffeine exhibits anti-fibrotic effect against hypoxia-induced renal fibroblast activation through its antioxidant property to eliminate intracellular ROS, at least in part, via downstream catalase and Nrf2 mechanisms. |
topic |
catalase coffee fibrogenesis hypoxia nrf2 renal fibrosis ros |
url |
http://dx.doi.org/10.1080/19336918.2019.1638691 |
work_keys_str_mv |
AT angkhananilnumkhum caffeineinhibitshypoxiainducedrenalfibroblastactivationbyantioxidantmechanism AT rattiyapornkanlaya caffeineinhibitshypoxiainducedrenalfibroblastactivationbyantioxidantmechanism AT sunisayoodee caffeineinhibitshypoxiainducedrenalfibroblastactivationbyantioxidantmechanism AT visiththongboonkerd caffeineinhibitshypoxiainducedrenalfibroblastactivationbyantioxidantmechanism |
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1725243375861366784 |