Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.)
Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures...
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Iranian Medicinal Plants Society
2013-02-01
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Series: | Journal of Medicinal Plants and By-products |
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Online Access: | http://jmpb.areeo.ac.ir/article_108592_66458840d1bc6880c00e0fa0c4b47009.pdf |
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doaj-0f98e1e6d25b412d858be6849960ca282020-11-25T00:41:12ZengIranian Medicinal Plants SocietyJournal of Medicinal Plants and By-products2322-13992588-37392013-02-0122177184108592Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.)Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after 72 h. In the cultures stimulated by zinc nano-oxide, the hypericin and hyperforin production reached to the maximum (7.87 and 217.45 µg/g DW, respectively), which were 3 and 13-fold higher than the control. The amount of hypericin and hyperforin was increased from 2.07 and 16.27 µg/g DW to 11.18 and 195.62 µg/g DW in iron nano-oxide treated cultures. The cell cultures treated with zinc and iron nano-oxides showed increased hyperforin production as compared to the hypericin production. These observations suggested that nano-particles can be appropriate candidates for elicitation studies of in vitro secondary metabolites production.http://jmpb.areeo.ac.ir/article_108592_66458840d1bc6880c00e0fa0c4b47009.pdfCell suspension. Hypericum perfuratum. Hypericin. Hyperforin. Nano-particles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
title |
Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.) |
spellingShingle |
Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.) Journal of Medicinal Plants and By-products Cell suspension. Hypericum perfuratum. Hypericin. Hyperforin. Nano-particles |
title_short |
Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.) |
title_full |
Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.) |
title_fullStr |
Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.) |
title_full_unstemmed |
Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.) |
title_sort |
improvement of hypericin and hyperforin production using zinc and iron nano-oxides as elicitors in cell suspension culture of st john's wort (hypericum perforatum l.) |
publisher |
Iranian Medicinal Plants Society |
series |
Journal of Medicinal Plants and By-products |
issn |
2322-1399 2588-3739 |
publishDate |
2013-02-01 |
description |
Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after 72 h. In the cultures stimulated by zinc nano-oxide, the hypericin and hyperforin production reached to the maximum (7.87 and 217.45 µg/g DW, respectively), which were 3 and 13-fold higher than the control. The amount of hypericin and hyperforin was increased from 2.07 and 16.27 µg/g DW to 11.18 and 195.62 µg/g DW in iron nano-oxide treated cultures. The cell cultures treated with zinc and iron nano-oxides showed increased hyperforin production as compared to the hypericin production. These observations suggested that nano-particles can be appropriate candidates for elicitation studies of in vitro secondary metabolites production. |
topic |
Cell suspension. Hypericum perfuratum. Hypericin. Hyperforin. Nano-particles |
url |
http://jmpb.areeo.ac.ir/article_108592_66458840d1bc6880c00e0fa0c4b47009.pdf |
_version_ |
1725286728875376640 |