Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)

Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures...

Full description

Bibliographic Details
Format: Article
Language:English
Published: Iranian Medicinal Plants Society 2013-02-01
Series:Journal of Medicinal Plants and By-products
Subjects:
Online Access:http://jmpb.areeo.ac.ir/article_108592_66458840d1bc6880c00e0fa0c4b47009.pdf
id doaj-0f98e1e6d25b412d858be6849960ca28
record_format Article
spelling doaj-0f98e1e6d25b412d858be6849960ca282020-11-25T00:41:12ZengIranian Medicinal Plants SocietyJournal of Medicinal Plants and By-products2322-13992588-37392013-02-0122177184108592Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after 72 h. In the cultures stimulated by zinc nano-oxide, the hypericin and hyperforin production reached to the maximum (7.87 and 217.45 µg/g DW, respectively), which were 3 and 13-fold higher than the control. The amount of hypericin and hyperforin was increased from 2.07 and 16.27 µg/g DW to 11.18 and 195.62 µg/g DW in iron nano-oxide treated cultures. The cell cultures treated with zinc and iron nano-oxides showed increased hyperforin production as compared to the hypericin production. These observations suggested that nano-particles can be appropriate candidates for elicitation studies of in vitro secondary metabolites production.http://jmpb.areeo.ac.ir/article_108592_66458840d1bc6880c00e0fa0c4b47009.pdfCell suspension. Hypericum perfuratum. Hypericin. Hyperforin. Nano-particles
collection DOAJ
language English
format Article
sources DOAJ
title Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)
spellingShingle Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)
Journal of Medicinal Plants and By-products
Cell suspension. Hypericum perfuratum. Hypericin. Hyperforin. Nano-particles
title_short Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)
title_full Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)
title_fullStr Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)
title_full_unstemmed Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell‏ Suspension Culture of St John's wort (Hypericum perforatum L.)
title_sort improvement of hypericin and hyperforin production using zinc and iron nano-oxides as elicitors in cell‏ suspension culture of st john's wort (hypericum perforatum l.)
publisher Iranian Medicinal Plants Society
series Journal of Medicinal Plants and By-products
issn 2322-1399
2588-3739
publishDate 2013-02-01
description Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after 72 h. In the cultures stimulated by zinc nano-oxide, the hypericin and hyperforin production reached to the maximum (7.87 and 217.45 µg/g DW, respectively), which were 3 and 13-fold higher than the control. The amount of hypericin and hyperforin was increased from 2.07 and 16.27 µg/g DW to 11.18 and 195.62 µg/g DW in iron nano-oxide treated cultures. The cell cultures treated with zinc and iron nano-oxides showed increased hyperforin production as compared to the hypericin production. These observations suggested that nano-particles can be appropriate candidates for elicitation studies of in vitro secondary metabolites production.
topic Cell suspension. Hypericum perfuratum. Hypericin. Hyperforin. Nano-particles
url http://jmpb.areeo.ac.ir/article_108592_66458840d1bc6880c00e0fa0c4b47009.pdf
_version_ 1725286728875376640