High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow
Hematopoiesis is hosted, supported and regulated by a special bone marrow (BM) microenvironment known as “niche.” BM niches have been classified based on micro-anatomic distance from the bone surface into “endosteal” and “central” niches. Whilst different blood vessels have been found in both BM nic...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-12-01
|
Series: | Frontiers in Cell and Developmental Biology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2020.596452/full |
id |
doaj-0f9dbc5f22c542d88d0ba5e0ba58b7f7 |
---|---|
record_format |
Article |
spelling |
doaj-0f9dbc5f22c542d88d0ba5e0ba58b7f72020-12-08T06:03:06ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-12-01810.3389/fcell.2020.596452596452High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone MarrowFrancesca M. Panvini0Francesca M. Panvini1Simone Pacini2Marina Montali3Serena Barachini4Stefano Mazzoni5Riccardo Morganti6Eugenio M. Ciancia7Vittoria Carnicelli8Mario Petrini9Institute of Life Sciences, Sant’Anna School of Advanced Studies, Pisa, ItalyDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, ItalyDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, ItalyDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, ItalyDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, ItalyDepartment of Translational Research and New Technology in Medicine, University of Pisa, Pisa, ItalyStatistical Support to Clinical Trials Department, Azienda Ospedaliero Universitaria Pisana, Pisa, ItalyDepartment of Pathology, Azienda Ospedsaliero Universitaria Pisana, Pisa, ItalyDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, ItalyDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, ItalyHematopoiesis is hosted, supported and regulated by a special bone marrow (BM) microenvironment known as “niche.” BM niches have been classified based on micro-anatomic distance from the bone surface into “endosteal” and “central” niches. Whilst different blood vessels have been found in both BM niches in mice, our knowledge of the human BM architecture is much more limited. Here, we have used a combination of markers including NESTIN, CD146, and αSMA labeling different blood vessels in benign human BM. Applying immunohistochemical/immunofluorescence techniques on BM trephines and performing image analysis on almost 300 microphotographs, we detected high NESTIN expression in BM endothelial cells (BMECs) of small arteries (A) and endosteal arterioles (EA), and also in very small vessels we named NESTIN+ capillary-like tubes (NCLTs), not surrounded by sub-endothelial perivascular cells that occasionally reported low levels of NESTIN expression. Statistically, NCLTs were detected within 40 μm from bone trabecula, frequently found in direct contact to the bone line and spatially correlated with hematopoietic stem/progenitor cells. Our results support the expression of NESTIN in human BMECs of EA and A in accordance with the updated classification of murine BM micro-vessels. NCLTs for their peculiar characteristics and micro-anatomical localization have been here proposed as transitional vessels possibly involved in regulating human hematopoiesis.https://www.frontiersin.org/articles/10.3389/fcell.2020.596452/fullbone marrowmicro-vesselsnicheNESTINhematopoietic stem cellsendosteum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Francesca M. Panvini Francesca M. Panvini Simone Pacini Marina Montali Serena Barachini Stefano Mazzoni Riccardo Morganti Eugenio M. Ciancia Vittoria Carnicelli Mario Petrini |
spellingShingle |
Francesca M. Panvini Francesca M. Panvini Simone Pacini Marina Montali Serena Barachini Stefano Mazzoni Riccardo Morganti Eugenio M. Ciancia Vittoria Carnicelli Mario Petrini High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow Frontiers in Cell and Developmental Biology bone marrow micro-vessels niche NESTIN hematopoietic stem cells endosteum |
author_facet |
Francesca M. Panvini Francesca M. Panvini Simone Pacini Marina Montali Serena Barachini Stefano Mazzoni Riccardo Morganti Eugenio M. Ciancia Vittoria Carnicelli Mario Petrini |
author_sort |
Francesca M. Panvini |
title |
High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow |
title_short |
High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow |
title_full |
High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow |
title_fullStr |
High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow |
title_full_unstemmed |
High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow |
title_sort |
high nestin expression marks the endosteal capillary network in human bone marrow |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-12-01 |
description |
Hematopoiesis is hosted, supported and regulated by a special bone marrow (BM) microenvironment known as “niche.” BM niches have been classified based on micro-anatomic distance from the bone surface into “endosteal” and “central” niches. Whilst different blood vessels have been found in both BM niches in mice, our knowledge of the human BM architecture is much more limited. Here, we have used a combination of markers including NESTIN, CD146, and αSMA labeling different blood vessels in benign human BM. Applying immunohistochemical/immunofluorescence techniques on BM trephines and performing image analysis on almost 300 microphotographs, we detected high NESTIN expression in BM endothelial cells (BMECs) of small arteries (A) and endosteal arterioles (EA), and also in very small vessels we named NESTIN+ capillary-like tubes (NCLTs), not surrounded by sub-endothelial perivascular cells that occasionally reported low levels of NESTIN expression. Statistically, NCLTs were detected within 40 μm from bone trabecula, frequently found in direct contact to the bone line and spatially correlated with hematopoietic stem/progenitor cells. Our results support the expression of NESTIN in human BMECs of EA and A in accordance with the updated classification of murine BM micro-vessels. NCLTs for their peculiar characteristics and micro-anatomical localization have been here proposed as transitional vessels possibly involved in regulating human hematopoiesis. |
topic |
bone marrow micro-vessels niche NESTIN hematopoietic stem cells endosteum |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2020.596452/full |
work_keys_str_mv |
AT francescampanvini highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT francescampanvini highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT simonepacini highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT marinamontali highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT serenabarachini highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT stefanomazzoni highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT riccardomorganti highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT eugeniomciancia highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT vittoriacarnicelli highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow AT mariopetrini highnestinexpressionmarkstheendostealcapillarynetworkinhumanbonemarrow |
_version_ |
1724391482786316288 |