REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
The aim of this study was to follow the contamination of food with Listeria monocytogenes by using Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and SensiFAST SYBR Hi-ROX Kit for the real-time PCR performance. In 24 sam...
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Slovak University of Agriculture
2013-02-01
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doaj-0f9fdde507004884843841d2fcb3bceb2020-11-25T01:17:02ZengSlovak University of AgricultureJournal of Microbiology, Biotechnology and Food Sciences1338-51782013-02-012Special issue15501558REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGINJaroslav PochopMiroslava KačániováLukáš HlebaJana PetrováĽubomír LopašovskýAdriana PavelkováAlica BobkováThe aim of this study was to follow the contamination of food with Listeria monocytogenes by using Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and SensiFAST SYBR Hi-ROX Kit for the real-time PCR performance. In 24 samples of food of animal origin without incubation were detected strains of Listeria monocytogenes in 15 samples (swabs). Nine samples were negative. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in food of animal origin without incubation. This could prevent infection caused by Listeria monocytogenes, and also could benefit food manufacturing companies by extending their product’s shelf-life as well as saving the cost of warehousing their food products while awaiting pathogen testing results. The rapid real-time PCR-based method performed very well compared to the conventional method. It is a fast, simple, specific and sensitive way to detect nucleic acids, which could be used in clinical diagnostic tests in the future. http://www.jmbfs.org/wp-content/uploads/2013/06/55_jmbs_pochop_fbp_m.pdfReal-time PCRListeria monocytogenesdetection kitready-to-eat food |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jaroslav Pochop Miroslava Kačániová Lukáš Hleba Jana Petrová Ľubomír Lopašovský Adriana Pavelková Alica Bobková |
spellingShingle |
Jaroslav Pochop Miroslava Kačániová Lukáš Hleba Jana Petrová Ľubomír Lopašovský Adriana Pavelková Alica Bobková REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN Journal of Microbiology, Biotechnology and Food Sciences Real-time PCR Listeria monocytogenes detection kit ready-to-eat food |
author_facet |
Jaroslav Pochop Miroslava Kačániová Lukáš Hleba Jana Petrová Ľubomír Lopašovský Adriana Pavelková Alica Bobková |
author_sort |
Jaroslav Pochop |
title |
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN |
title_short |
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN |
title_full |
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN |
title_fullStr |
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN |
title_full_unstemmed |
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN |
title_sort |
real-time pcr detection of listeria monocytogenes in food samples of animal origin |
publisher |
Slovak University of Agriculture |
series |
Journal of Microbiology, Biotechnology and Food Sciences |
issn |
1338-5178 |
publishDate |
2013-02-01 |
description |
The aim of this study was to follow the contamination of food with Listeria monocytogenes by using Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and SensiFAST SYBR Hi-ROX Kit for the real-time PCR performance. In 24 samples of food of animal origin without incubation were detected strains of Listeria monocytogenes in 15 samples (swabs). Nine samples were negative. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in food of animal origin without incubation. This could prevent infection caused by Listeria monocytogenes, and also could benefit food manufacturing companies by extending their product’s shelf-life as well as saving the cost of warehousing their food products while awaiting pathogen testing results. The rapid real-time PCR-based method performed very well compared to the conventional method. It is a fast, simple, specific and sensitive way to detect nucleic acids, which could be used in clinical diagnostic tests in the future. |
topic |
Real-time PCR Listeria monocytogenes detection kit ready-to-eat food |
url |
http://www.jmbfs.org/wp-content/uploads/2013/06/55_jmbs_pochop_fbp_m.pdf |
work_keys_str_mv |
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