Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts
Polycyclic aromatic hydrocarbons are known carcinogens used in rodent experimental models. In this study, the carcinogen DMBA (7,12-dimethylbenzanthracene) was administered by gavage, diluted in corn oil, to female BALB / c mice at hebdomadary doses of 1 mg per animal for 1, 3, 6 or 9 weeks. Animal...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Universidade de São Paulo
2015-06-01
|
Series: | Brazilian Journal of Veterinary Research and Animal Science |
Subjects: | |
Online Access: | http://www.revistas.usp.br/bjvras/article/view/59426 |
id |
doaj-0fa661062c834c3c8f1bbf6fa76703ac |
---|---|
record_format |
Article |
spelling |
doaj-0fa661062c834c3c8f1bbf6fa76703ac2020-11-25T02:58:00ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562015-06-0152210.11606/issn.1678-4456.v52i2p125-13388351Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new factsKrishna Duro de Oliveira0Gabriela Uliana Avanzo1Marcello Vannucci Tedardi2Marcelo Monte Mór Rangel3José Luis Avanzo4Heidge Fukumasu5Kurapati Venkata Kesava Rao6Idércio Luiz Sinhorini7Maria Lúcia Zaidan Dagli8Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de PatologiaUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de PatologiaUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de PatologiaUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de PatologiaUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de PatologiaUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de AlimentosUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de PatologiaUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de PatologiaUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia Polycyclic aromatic hydrocarbons are known carcinogens used in rodent experimental models. In this study, the carcinogen DMBA (7,12-dimethylbenzanthracene) was administered by gavage, diluted in corn oil, to female BALB / c mice at hebdomadary doses of 1 mg per animal for 1, 3, 6 or 9 weeks. Animals were weighed and monitored weekly until death. Remaining animals were euthanized at the age of 53 weeks. At necropsy, representative fragments of neoplasms were collected and routinely processed for histopathological analysis. Of all mice that received DMBA, 68.57% developed some type of tumor. Of the 70 mice treated with various doses of DMBA, 22 (31.43%) developed mammary tumors. The adenoacanthoma was the most commonly (18.75%) diagnosed histological type of breast cancer. Lung (15.71%), lymphoid tissue (11.43%), stomach (7.14%) and skin (2.86%) were also primary sites of tumor development. One third (33.33%) of the mice receiving 1 mg of DMBA developed lung cancer. Therefore, the administration of DMBA was shown to be an efficient model of carcinogenesis in mice, especially for the study of breast cancer, when using the highest dose, and lung, when using the lowest dose. Carcinogenesis models have been used for several purposes in cancer research. These results represent new facts for a classic carcinogenesis model. http://www.revistas.usp.br/bjvras/article/view/59426CarcinogenesisDMBAMiceLung neoplasmsBreast neoplasms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Krishna Duro de Oliveira Gabriela Uliana Avanzo Marcello Vannucci Tedardi Marcelo Monte Mór Rangel José Luis Avanzo Heidge Fukumasu Kurapati Venkata Kesava Rao Idércio Luiz Sinhorini Maria Lúcia Zaidan Dagli |
spellingShingle |
Krishna Duro de Oliveira Gabriela Uliana Avanzo Marcello Vannucci Tedardi Marcelo Monte Mór Rangel José Luis Avanzo Heidge Fukumasu Kurapati Venkata Kesava Rao Idércio Luiz Sinhorini Maria Lúcia Zaidan Dagli Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts Brazilian Journal of Veterinary Research and Animal Science Carcinogenesis DMBA Mice Lung neoplasms Breast neoplasms |
author_facet |
Krishna Duro de Oliveira Gabriela Uliana Avanzo Marcello Vannucci Tedardi Marcelo Monte Mór Rangel José Luis Avanzo Heidge Fukumasu Kurapati Venkata Kesava Rao Idércio Luiz Sinhorini Maria Lúcia Zaidan Dagli |
author_sort |
Krishna Duro de Oliveira |
title |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_short |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_full |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_fullStr |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_full_unstemmed |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_sort |
chemical carcinogenesis by dmba (7,12-dimethylbenzanthracene) in female balb/c mice: new facts |
publisher |
Universidade de São Paulo |
series |
Brazilian Journal of Veterinary Research and Animal Science |
issn |
1413-9596 1678-4456 |
publishDate |
2015-06-01 |
description |
Polycyclic aromatic hydrocarbons are known carcinogens used in rodent experimental models. In this study, the carcinogen DMBA (7,12-dimethylbenzanthracene) was administered by gavage, diluted in corn oil, to female BALB / c mice at hebdomadary doses of 1 mg per animal for 1, 3, 6 or 9 weeks. Animals were weighed and monitored weekly until death. Remaining animals were euthanized at the age of 53 weeks. At necropsy, representative fragments of neoplasms were collected and routinely processed for histopathological analysis. Of all mice that received DMBA, 68.57% developed some type of tumor. Of the 70 mice treated with various doses of DMBA, 22 (31.43%) developed mammary tumors. The adenoacanthoma was the most commonly (18.75%) diagnosed histological type of breast cancer. Lung (15.71%), lymphoid tissue (11.43%), stomach (7.14%) and skin (2.86%) were also primary sites of tumor development. One third (33.33%) of the mice receiving 1 mg of DMBA developed lung cancer. Therefore, the administration of DMBA was shown to be an efficient model of carcinogenesis in mice, especially for the study of breast cancer, when using the highest dose, and lung, when using the lowest dose. Carcinogenesis models have been used for several purposes in cancer research. These results represent new facts for a classic carcinogenesis model.
|
topic |
Carcinogenesis DMBA Mice Lung neoplasms Breast neoplasms |
url |
http://www.revistas.usp.br/bjvras/article/view/59426 |
work_keys_str_mv |
AT krishnadurodeoliveira chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT gabrielaulianaavanzo chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT marcellovannuccitedardi chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT marcelomontemorrangel chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT joseluisavanzo chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT heidgefukumasu chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT kurapativenkatakesavarao chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT idercioluizsinhorini chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts AT marialuciazaidandagli chemicalcarcinogenesisbydmba712dimethylbenzanthraceneinfemalebalbcmicenewfacts |
_version_ |
1724709058569568256 |