The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence.
Telomerase RNAs (TERs) are highly divergent between species, varying in size and sequence composition. Here, we identify a candidate for the telomerase RNA component of Leishmania genus, which includes species that cause leishmaniasis, a neglected tropical disease. Merging a thorough computational s...
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doaj-0fbee470592341d6babecfbfc15bfc4d2020-11-25T01:17:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11206110.1371/journal.pone.0112061The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence.Elton J R VasconcelosVinícius S NunesMarcelo S da SilvaMarcela SegattoPeter J MylerMaria Isabel N CanoTelomerase RNAs (TERs) are highly divergent between species, varying in size and sequence composition. Here, we identify a candidate for the telomerase RNA component of Leishmania genus, which includes species that cause leishmaniasis, a neglected tropical disease. Merging a thorough computational screening combined with RNA-seq evidence, we mapped a non-coding RNA gene localized in a syntenic locus on chromosome 25 of five Leishmania species that shares partial synteny with both Trypanosoma brucei TER locus and a putative TER candidate-containing locus of Crithidia fasciculata. Using target-driven molecular biology approaches, we detected a ∼2,100 nt transcript (LeishTER) that contains a 5' spliced leader (SL) cap, a putative 3' polyA tail and a predicted C/D box snoRNA domain. LeishTER is expressed at similar levels in the logarithmic and stationary growth phases of promastigote forms. A 5'SL capped LeishTER co-immunoprecipitated and co-localized with the telomerase protein component (TERT) in a cell cycle-dependent manner. Prediction of its secondary structure strongly suggests the existence of a bona fide single-stranded template sequence and a conserved C[U/C]GUCA motif-containing helix II, representing the template boundary element. This study paves the way for further investigations on the biogenesis of parasite TERT ribonucleoproteins (RNPs) and its role in parasite telomere biology.http://europepmc.org/articles/PMC4229120?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elton J R Vasconcelos Vinícius S Nunes Marcelo S da Silva Marcela Segatto Peter J Myler Maria Isabel N Cano |
spellingShingle |
Elton J R Vasconcelos Vinícius S Nunes Marcelo S da Silva Marcela Segatto Peter J Myler Maria Isabel N Cano The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence. PLoS ONE |
author_facet |
Elton J R Vasconcelos Vinícius S Nunes Marcelo S da Silva Marcela Segatto Peter J Myler Maria Isabel N Cano |
author_sort |
Elton J R Vasconcelos |
title |
The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence. |
title_short |
The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence. |
title_full |
The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence. |
title_fullStr |
The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence. |
title_full_unstemmed |
The putative Leishmania telomerase RNA (LeishTER) undergoes trans-splicing and contains a conserved template sequence. |
title_sort |
putative leishmania telomerase rna (leishter) undergoes trans-splicing and contains a conserved template sequence. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Telomerase RNAs (TERs) are highly divergent between species, varying in size and sequence composition. Here, we identify a candidate for the telomerase RNA component of Leishmania genus, which includes species that cause leishmaniasis, a neglected tropical disease. Merging a thorough computational screening combined with RNA-seq evidence, we mapped a non-coding RNA gene localized in a syntenic locus on chromosome 25 of five Leishmania species that shares partial synteny with both Trypanosoma brucei TER locus and a putative TER candidate-containing locus of Crithidia fasciculata. Using target-driven molecular biology approaches, we detected a ∼2,100 nt transcript (LeishTER) that contains a 5' spliced leader (SL) cap, a putative 3' polyA tail and a predicted C/D box snoRNA domain. LeishTER is expressed at similar levels in the logarithmic and stationary growth phases of promastigote forms. A 5'SL capped LeishTER co-immunoprecipitated and co-localized with the telomerase protein component (TERT) in a cell cycle-dependent manner. Prediction of its secondary structure strongly suggests the existence of a bona fide single-stranded template sequence and a conserved C[U/C]GUCA motif-containing helix II, representing the template boundary element. This study paves the way for further investigations on the biogenesis of parasite TERT ribonucleoproteins (RNPs) and its role in parasite telomere biology. |
url |
http://europepmc.org/articles/PMC4229120?pdf=render |
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