Production of hydroxy marilone C as a bioactive compound from
Hydroxy marilone C is a bioactive metabolite produced from the culture broth of Streptomyces badius isolated from Egyptian soil. Hydroxy marilone C was purified and fractionated by a silica gel column with a gradient mobile phase dichloromethane (DCM):methanol then Sephadex LH-20 column using methan...
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doaj-0fd80cc70fd34c2da252b21d27270c0c2020-11-25T02:40:32ZengSpringerOpenJournal of Genetic Engineering and Biotechnology1687-157X2016-06-0114116116810.1016/j.jgeb.2016.04.001Production of hydroxy marilone C as a bioactive compound fromOsama H. El Sayed0Mohsen M.S. Asker1Mahmoud A. Swelim2Ibrahim H. Abbas3Aziza I. Attwa4Mohamed E. El Awady5Department of Microbial Biotechnology, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, EgyptDepartment of Microbial Biotechnology, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, EgyptBotany Department, Faculty of Science, Benha University, Benha, EgyptBotany Department, Faculty of Science, Benha University, Benha, EgyptDepartment of Microbial Biotechnology, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, EgyptDepartment of Microbial Biotechnology, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, EgyptHydroxy marilone C is a bioactive metabolite produced from the culture broth of Streptomyces badius isolated from Egyptian soil. Hydroxy marilone C was purified and fractionated by a silica gel column with a gradient mobile phase dichloromethane (DCM):methanol then Sephadex LH-20 column using methanol as a mobile phase. It was subjected to many procedures such as infrared (IR), nuclear magnetic resonance (NMR), Mass spectroscopy (MS) and UV spectroscopy for elucidation of its structure. It was evaluated for antioxidant, cytotoxicity against human alveolar basal epithelial cell line (A-549) and human breast adenocarcinoma cell line (MCF-7) and antiviral activities; showed that the maximum antioxidant activity was 78.8% at 3 mg/ml after 90 min. and the IC50 value against DPPH radical found about 1.5 mg/ml after 60 min. Using MTT assay the effect of the pure compound on the proliferation of A-549 cells and MCF-7 cells was 443 μg/ml and 147.9 μg/ml, respectively, while for detection of antiviral activity using Madin–Darby canine kidney (MDCK) cells the maximum cytotoxicity was at 27.9% and IC50 was 128.1 μg/ml. The maximum concentration required for protecting 50% of the virus-infected cells against H1N1 viral cytopathogenicity (EC50) was 33.25% for 80 μg/ml. These results indicated that the hydroxy marilone C has potential antitumor and antiviral activities.http://www.sciencedirect.com/science/article/pii/S1687157X16300063Streptomyces badiusHydroxy marilone CAnticancerAntiviral and antioxidant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Osama H. El Sayed Mohsen M.S. Asker Mahmoud A. Swelim Ibrahim H. Abbas Aziza I. Attwa Mohamed E. El Awady |
spellingShingle |
Osama H. El Sayed Mohsen M.S. Asker Mahmoud A. Swelim Ibrahim H. Abbas Aziza I. Attwa Mohamed E. El Awady Production of hydroxy marilone C as a bioactive compound from Journal of Genetic Engineering and Biotechnology Streptomyces badius Hydroxy marilone C Anticancer Antiviral and antioxidant |
author_facet |
Osama H. El Sayed Mohsen M.S. Asker Mahmoud A. Swelim Ibrahim H. Abbas Aziza I. Attwa Mohamed E. El Awady |
author_sort |
Osama H. El Sayed |
title |
Production of hydroxy marilone C as a bioactive compound from |
title_short |
Production of hydroxy marilone C as a bioactive compound from |
title_full |
Production of hydroxy marilone C as a bioactive compound from |
title_fullStr |
Production of hydroxy marilone C as a bioactive compound from |
title_full_unstemmed |
Production of hydroxy marilone C as a bioactive compound from |
title_sort |
production of hydroxy marilone c as a bioactive compound from |
publisher |
SpringerOpen |
series |
Journal of Genetic Engineering and Biotechnology |
issn |
1687-157X |
publishDate |
2016-06-01 |
description |
Hydroxy marilone C is a bioactive metabolite produced from the culture broth of Streptomyces badius isolated from Egyptian soil. Hydroxy marilone C was purified and fractionated by a silica gel column with a gradient mobile phase dichloromethane (DCM):methanol then Sephadex LH-20 column using methanol as a mobile phase. It was subjected to many procedures such as infrared (IR), nuclear magnetic resonance (NMR), Mass spectroscopy (MS) and UV spectroscopy for elucidation of its structure. It was evaluated for antioxidant, cytotoxicity against human alveolar basal epithelial cell line (A-549) and human breast adenocarcinoma cell line (MCF-7) and antiviral activities; showed that the maximum antioxidant activity was 78.8% at 3 mg/ml after 90 min. and the IC50 value against DPPH radical found about 1.5 mg/ml after 60 min. Using MTT assay the effect of the pure compound on the proliferation of A-549 cells and MCF-7 cells was 443 μg/ml and 147.9 μg/ml, respectively, while for detection of antiviral activity using Madin–Darby canine kidney (MDCK) cells the maximum cytotoxicity was at 27.9% and IC50 was 128.1 μg/ml. The maximum concentration required for protecting 50% of the virus-infected cells against H1N1 viral cytopathogenicity (EC50) was 33.25% for 80 μg/ml. These results indicated that the hydroxy marilone C has potential antitumor and antiviral activities. |
topic |
Streptomyces badius Hydroxy marilone C Anticancer Antiviral and antioxidant |
url |
http://www.sciencedirect.com/science/article/pii/S1687157X16300063 |
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