Tracking transparent monogenean parasites on fish from infection to maturity
The infection dynamics and distribution of the ectoparasitic fish monogenean Neobenedenia sp. (Monogenea: Capsalidae) throughout its development was examined on barramundi, Lates calcarifer (Bloch) (Latidae), by labelling transparent, ciliated larvae (oncomiracidia) with a fluorescent dye. Replicate...
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doaj-0fe71771eb9b4e0586602f223b2d44dc2020-11-25T00:59:46ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442015-12-014331632210.1016/j.ijppaw.2015.06.002Tracking transparent monogenean parasites on fish from infection to maturityAlejandro Trujillo-González0Constantin C. Constantinoiu1Richard Rowe2Kate S. Hutson3Marine Parasitology Laboratory, Centre for Sustainable Tropical Fisheries and Aquaculture, College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, AustraliaCentre for Biosecurity in Tropical Infectious Diseases, College of Public Health, Medical and Vet Sciences, James Cook University, Townsville, Queensland, AustraliaCollege of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, AustraliaMarine Parasitology Laboratory, Centre for Sustainable Tropical Fisheries and Aquaculture, College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, AustraliaThe infection dynamics and distribution of the ectoparasitic fish monogenean Neobenedenia sp. (Monogenea: Capsalidae) throughout its development was examined on barramundi, Lates calcarifer (Bloch) (Latidae), by labelling transparent, ciliated larvae (oncomiracidia) with a fluorescent dye. Replicate fish were each exposed to approximately 50 fluorescent oncomiracidia and then examined for parasites using an epifluorescence stereomicroscope at 10 time intervals post-exposure (15, 30, 60, 120 min, 24, 48 h, four, eight, 12, and 16 days). Fluorescent labelling revealed that parasites attached underneath and on the surface of the scales of host fish. Parasite infection success was 20% within 15 min, and peaked at 93% two days post-exposure, before gradually declining between four and sixteen days. Differences in parasite distribution on L. calcarifer over time provided strong evidence that Neobenedenia sp. larvae settled opportunistically and then migrated to specific microhabitats. Parasites initially attached (<24 h) in greater mean numbers on the body surface (13 ± 1.5) compared to the fins (4 ± 0.42) and head region (2 ± 0.41). Once larvae recruitment had ceased (48 h), there were significantly higher mean post-larvae counts on the head (5 ± 3.4) and fins (12 ± 3) compared to previous time intervals. Neobenedenia sp. aggregated on the eyes, fins, and dorsal and ventral extremities on the main body. As parasites neared sexual maturity, there was a marked aggregation on the fins (22 ± 2.35) compared to the head (4 ± 0.97) and body (9 ± 1.33), indicating that Neobenedenia sp. may form mating aggregations.http://www.sciencedirect.com/science/article/pii/S2213224415300043MonogeneaNeobenedeniaCapsalidaeDevelopmentMigrationMicrohabitatFluorescent labelling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alejandro Trujillo-González Constantin C. Constantinoiu Richard Rowe Kate S. Hutson |
spellingShingle |
Alejandro Trujillo-González Constantin C. Constantinoiu Richard Rowe Kate S. Hutson Tracking transparent monogenean parasites on fish from infection to maturity International Journal for Parasitology: Parasites and Wildlife Monogenea Neobenedenia Capsalidae Development Migration Microhabitat Fluorescent labelling |
author_facet |
Alejandro Trujillo-González Constantin C. Constantinoiu Richard Rowe Kate S. Hutson |
author_sort |
Alejandro Trujillo-González |
title |
Tracking transparent monogenean parasites on fish from infection to maturity |
title_short |
Tracking transparent monogenean parasites on fish from infection to maturity |
title_full |
Tracking transparent monogenean parasites on fish from infection to maturity |
title_fullStr |
Tracking transparent monogenean parasites on fish from infection to maturity |
title_full_unstemmed |
Tracking transparent monogenean parasites on fish from infection to maturity |
title_sort |
tracking transparent monogenean parasites on fish from infection to maturity |
publisher |
Elsevier |
series |
International Journal for Parasitology: Parasites and Wildlife |
issn |
2213-2244 |
publishDate |
2015-12-01 |
description |
The infection dynamics and distribution of the ectoparasitic fish monogenean Neobenedenia sp. (Monogenea: Capsalidae) throughout its development was examined on barramundi, Lates calcarifer (Bloch) (Latidae), by labelling transparent, ciliated larvae (oncomiracidia) with a fluorescent dye. Replicate fish were each exposed to approximately 50 fluorescent oncomiracidia and then examined for parasites using an epifluorescence stereomicroscope at 10 time intervals post-exposure (15, 30, 60, 120 min, 24, 48 h, four, eight, 12, and 16 days). Fluorescent labelling revealed that parasites attached underneath and on the surface of the scales of host fish. Parasite infection success was 20% within 15 min, and peaked at 93% two days post-exposure, before gradually declining between four and sixteen days. Differences in parasite distribution on L. calcarifer over time provided strong evidence that Neobenedenia sp. larvae settled opportunistically and then migrated to specific microhabitats. Parasites initially attached (<24 h) in greater mean numbers on the body surface (13 ± 1.5) compared to the fins (4 ± 0.42) and head region (2 ± 0.41). Once larvae recruitment had ceased (48 h), there were significantly higher mean post-larvae counts on the head (5 ± 3.4) and fins (12 ± 3) compared to previous time intervals. Neobenedenia sp. aggregated on the eyes, fins, and dorsal and ventral extremities on the main body. As parasites neared sexual maturity, there was a marked aggregation on the fins (22 ± 2.35) compared to the head (4 ± 0.97) and body (9 ± 1.33), indicating that Neobenedenia sp. may form mating aggregations. |
topic |
Monogenea Neobenedenia Capsalidae Development Migration Microhabitat Fluorescent labelling |
url |
http://www.sciencedirect.com/science/article/pii/S2213224415300043 |
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