The effect of endo- and exogenous factors on growth and maturation of oilseed rape (Brassica napus cv. Jet Neuf) siliques cultured in vitro

• Main Authors: Lucyna Drozdowska, Małgorzata Ulanowska, Janina Rogozińska
• Format: Article
• Language: English
• Published: Polish Botanical Society 2014-01-01
• Series: Acta Societatis Botanicorum Poloniae
• Subjects: Brassica napus; silique growth in vitro; growth regulators
• Online Access: https://pbsociety.org.pl/journals/index.php/asbp/article/view/1169

The effect of developmental stage of siliques, growth regulators, agar and sucrose concentrations in the medium was investigated on the morphogenesis of the silliques and seed maturation. Regeneration of shoots, roots and callus occurred at the base of siliques during their growth. The developmental stage of the incubated siliques was the most important factor affecting the number of seeds, while the conditions of culture (agar and growth regulators) constituted a secondary factor. The reasons for the insignificant number of seeds produced could be the lack of fertilization of some of the ovules, limited ability of siliques to take up nutrients and the unspecific composition of the medium.