Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite Instability
Microsatellite instability (MSI) is a molecular phenotype due to a deficient DNA mismatch repair (dMMR). In colorectal cancer (CRC), dMMR/MSI is associated with several clinical and histopathological features, influences prognosis, and is a predictive factor of response to therapy. In daily practice...
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doaj-0ffab4d586e64647893eb8e781ae5efc2021-01-30T00:03:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01221340134010.3390/ijms22031340Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite InstabilityBeatriz Leão0Xiaogang Wen1Henrique O. Duarte2Irene Gullo3Gilza Gonçalves4Patrícia Pontes5Claudia Castelli6Francisca Diniz7Stefan Mereiter8Joana Gomes9Fátima Carneiro10Celso A. Reis11Faculty of Medicine, University of Porto, 4200-319 Porto, PortugalDepartment of Pathology, Centro Hospitalar Vila Nova de Gaia/Espinho, 4434-502 Vila Nova de Gaia, Portugali3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, PortugalFaculty of Medicine, University of Porto, 4200-319 Porto, PortugalIPATIMUP—Institute of Molecular Pathology and Immunology, University of Porto, 4200-135 Porto, PortugalIPATIMUP—Institute of Molecular Pathology and Immunology, University of Porto, 4200-135 Porto, PortugalDepartment of Diagnostics and Public Health, Section of Pathology, University and Hospital Trust of Verona, 37134 Verona, Italyi3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, PortugalFaculty of Medicine, University of Porto, 4200-319 Porto, PortugalFaculty of Medicine, University of Porto, 4200-319 Porto, PortugalMicrosatellite instability (MSI) is a molecular phenotype due to a deficient DNA mismatch repair (dMMR). In colorectal cancer (CRC), dMMR/MSI is associated with several clinical and histopathological features, influences prognosis, and is a predictive factor of response to therapy. In daily practice, dMMR/MSI profiles are identified by immunohistochemistry and/or multiplex PCR. The Thomsen–Friedenreich (TF) antigen was previously found to be a potential single marker to identify MSI-high gastric cancers. Therefore, in this study, we aimed to disclose a possible association between TF expression and MSI status in CRC. Furthermore, we evaluated the relationship between TF expression and other clinicopathological features, including patient survival. We evaluated the expression of the TF antigen in a cohort of 25 MSI-high and 71 microsatellite stable (MSS) CRCs. No association was observed between the expression of the TF antigen and MSI-high status in CRC. The survival analysis revealed that patients with MSI-high CRC showed improved survival when the TF antigen was expressed. This finding holds promise as it indicates the potential use of the TF antigen as a biomarker of better prognosis in MSI-high CRCs that should be validated in an independent and larger CRC cohort.https://www.mdpi.com/1422-0067/22/3/1340glycosylationcolorectal cancermicrosatellite instabilityThomsen–Friedenreich antigen<i>O</i>-glycan |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Beatriz Leão Xiaogang Wen Henrique O. Duarte Irene Gullo Gilza Gonçalves Patrícia Pontes Claudia Castelli Francisca Diniz Stefan Mereiter Joana Gomes Fátima Carneiro Celso A. Reis |
spellingShingle |
Beatriz Leão Xiaogang Wen Henrique O. Duarte Irene Gullo Gilza Gonçalves Patrícia Pontes Claudia Castelli Francisca Diniz Stefan Mereiter Joana Gomes Fátima Carneiro Celso A. Reis Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite Instability International Journal of Molecular Sciences glycosylation colorectal cancer microsatellite instability Thomsen–Friedenreich antigen <i>O</i>-glycan |
author_facet |
Beatriz Leão Xiaogang Wen Henrique O. Duarte Irene Gullo Gilza Gonçalves Patrícia Pontes Claudia Castelli Francisca Diniz Stefan Mereiter Joana Gomes Fátima Carneiro Celso A. Reis |
author_sort |
Beatriz Leão |
title |
Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite Instability |
title_short |
Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite Instability |
title_full |
Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite Instability |
title_fullStr |
Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite Instability |
title_full_unstemmed |
Expression of Thomsen–Friedenreich Antigen in Colorectal Cancer and Association with Microsatellite Instability |
title_sort |
expression of thomsen–friedenreich antigen in colorectal cancer and association with microsatellite instability |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-01-01 |
description |
Microsatellite instability (MSI) is a molecular phenotype due to a deficient DNA mismatch repair (dMMR). In colorectal cancer (CRC), dMMR/MSI is associated with several clinical and histopathological features, influences prognosis, and is a predictive factor of response to therapy. In daily practice, dMMR/MSI profiles are identified by immunohistochemistry and/or multiplex PCR. The Thomsen–Friedenreich (TF) antigen was previously found to be a potential single marker to identify MSI-high gastric cancers. Therefore, in this study, we aimed to disclose a possible association between TF expression and MSI status in CRC. Furthermore, we evaluated the relationship between TF expression and other clinicopathological features, including patient survival. We evaluated the expression of the TF antigen in a cohort of 25 MSI-high and 71 microsatellite stable (MSS) CRCs. No association was observed between the expression of the TF antigen and MSI-high status in CRC. The survival analysis revealed that patients with MSI-high CRC showed improved survival when the TF antigen was expressed. This finding holds promise as it indicates the potential use of the TF antigen as a biomarker of better prognosis in MSI-high CRCs that should be validated in an independent and larger CRC cohort. |
topic |
glycosylation colorectal cancer microsatellite instability Thomsen–Friedenreich antigen <i>O</i>-glycan |
url |
https://www.mdpi.com/1422-0067/22/3/1340 |
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