Preparation and quality by design assisted (Qb-d) optimization of bioceramic loaded microspheres for periodontal delivery of doxycycline hyclate

• Main Authors: Pooja Jain, Abhinav Garg, Uzma Farooq, Amulya K. Panda, Mohd. Aamir Mirza, Ahmed Noureldeen, Hadeer Darwish, Zeenat Iqbal
• Format: Article
• Language: English
• Published: Elsevier 2021-05-01
• Series: Saudi Journal of Biological Sciences
• Subjects: Bioceramic; Composite; Quality by design; Central composite design; Microspheres
• Online Access: http://www.sciencedirect.com/science/article/pii/S1319562X21002175

PLGA (Lactic- co-glycolic acid) coated chitosan microspheres loaded with hydroxyapatite and doxycycline hyclate complex were developed in the present study for periodontal delivery. A modified single emulsion method was adopted for the development of microspheres. Formulation was optimized on the basis of particle size, drug loading and encapsulation efficiency with the central composite design using 23 factorial design. Microspheres were optimized and electron microscopy revealed their spherical shape and porous nature. In-vitro study showed initial burst and then sustained release behavior of the formulation for 14 days. Further, in-vitro antibacterial study performed on E. coli (ATCC-25922) and S. aureus (ATCC-29213) revealed concentration dependent activity. Also, in-vitro cyto-toxicity assessment ensures biocompatibility of the formulation with the fibroblast’s cells. Overall, the quality by design assisted PLGA microspheres, demonstrated the desired attributes and were found suitable for periodontal drug delivery.