Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti
Introduction The effects of piperonyl butoxide (PBO) on the toxicity of the organophosphate temephos (TE) and the role of esterases in the resistance of Aedes aegypti to this insecticide were evaluated. Methods A. aegypti L4 larvae susceptible and resistant to TE were pre-treated with PBO solution...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2014-10-01
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doaj-10106f53215043ab9cce30c488e83a6a2020-11-24T22:55:09ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492014-10-0147557958210.1590/0037-8682-0195-2014S0037-86822014000500579Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegyptiBoscolli Barbosa PereiraJean Ezequiel LimongiEdimar Olegário de Campos JúniorDenis Prudencio LuizWarwick Estevam KerrIntroduction The effects of piperonyl butoxide (PBO) on the toxicity of the organophosphate temephos (TE) and the role of esterases in the resistance of Aedes aegypti to this insecticide were evaluated. Methods A. aegypti L4 larvae susceptible and resistant to TE were pre-treated with PBO solutions in acetone at concentrations of 0.125, 0.25, 0.5, 1, and 2% for 24h and subsequently exposed to a diagnostic concentration of 0.02mg/L aqueous TE solution. The esterase activity of the larvae extracts pre-treated with varying PBO concentrations and exposed to TE for three time periods was determined. Results At concentrations of 0.25, 0.5, 1, and 2%, PBO showed a significant synergistic effect with TE toxicity. High levels of esterase activity were associated with the survival of A. aegypti L4 larvae exposed to TE only. Conclusions The results of the biochemical assays suggest that PBO has a significant inhibitory effect on the total esterase activity in A. aegypti larvae.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500579&lng=en&tlng=enTemephosInsecticide resistanceAedes aegyptiEsterases |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Boscolli Barbosa Pereira Jean Ezequiel Limongi Edimar Olegário de Campos Júnior Denis Prudencio Luiz Warwick Estevam Kerr |
spellingShingle |
Boscolli Barbosa Pereira Jean Ezequiel Limongi Edimar Olegário de Campos Júnior Denis Prudencio Luiz Warwick Estevam Kerr Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti Revista da Sociedade Brasileira de Medicina Tropical Temephos Insecticide resistance Aedes aegypti Esterases |
author_facet |
Boscolli Barbosa Pereira Jean Ezequiel Limongi Edimar Olegário de Campos Júnior Denis Prudencio Luiz Warwick Estevam Kerr |
author_sort |
Boscolli Barbosa Pereira |
title |
Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti |
title_short |
Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti |
title_full |
Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti |
title_fullStr |
Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti |
title_full_unstemmed |
Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti |
title_sort |
effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of aedes aegypti |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
series |
Revista da Sociedade Brasileira de Medicina Tropical |
issn |
1678-9849 |
publishDate |
2014-10-01 |
description |
Introduction The effects of piperonyl butoxide (PBO) on the toxicity of the organophosphate temephos (TE) and the role of esterases in the resistance of Aedes aegypti to this insecticide were evaluated. Methods A. aegypti L4 larvae susceptible and resistant to TE were pre-treated with PBO solutions in acetone at concentrations of 0.125, 0.25, 0.5, 1, and 2% for 24h and subsequently exposed to a diagnostic concentration of 0.02mg/L aqueous TE solution. The esterase activity of the larvae extracts pre-treated with varying PBO concentrations and exposed to TE for three time periods was determined. Results At concentrations of 0.25, 0.5, 1, and 2%, PBO showed a significant synergistic effect with TE toxicity. High levels of esterase activity were associated with the survival of A. aegypti L4 larvae exposed to TE only. Conclusions The results of the biochemical assays suggest that PBO has a significant inhibitory effect on the total esterase activity in A. aegypti larvae. |
topic |
Temephos Insecticide resistance Aedes aegypti Esterases |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500579&lng=en&tlng=en |
work_keys_str_mv |
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